解淀粉芽孢杆菌SSY2颉颃霉菌物质的鉴定及其理化性质研究

doi:10.16431/j.cnki.1671-7236.2018.11.029

Abstract

Abstract:

The test was designed to identify the antifungal substances of Bacillus amyloliquefaciens SSY2 and their physicochemical properties.Identification and extraction of antibacterial substances were carried out by active component analysis,ammonium sulfate precipitation,ultrafiltration and salt removal;The physical and chemical properties were initially determined by detecting the time,enzyme activity,temperature,pH,stability and storage temperature of the mold material.The results showed that the degradation rate of different components to mould was different.The supernatant of bacterial liquid had the strongest degradation ability to mould,the degradation ability of bacterial liquid fermentation liquid was the second,and the degradation ability of intracellular liquid was the worst.Therefore,it was preliminarily determined that the active ingredient of the antibacterial substance of Bacillus amyloliquefaciens SSY2 was an extracellular secretion,which was mainly present in the supernatant of the bacterial liquid.After 30%,60%,80% and 100% saturated ammonium sulfate precipitation,the optimum ammonium sulfate saturation concentration was determined to be 60%;After ultrafiltration purification,an antibacterial active substances with molecular mass greater than 3 ku was obtained.The results of the physical and chemical property test showed that,the active substance was produced early (24 h) in the logarithmic phase,and it had good passage stability.It had protease activity and resistance to pepsin,but it had lower resistance to protease K,alkaline and neutral protease;The antibacterial activity at 60 to 121℃ was stable,which proved that it was not sensitive to heat;There was no significant difference in the antibacterial activity of the supernatant against molds in pH 6.0 to 8.0 environment,it was proved that antimicrobial substances had strong acid resistance;Antimicrobial substances had good passage stability,and it could be stored at 4℃ for a long time.The results could provide basis for further research and development of fungal biological control agents.

Key words: Bacillus amyloliquefaciens; physicochemical property; mould

CLC Number:

S816.79

CHEN Nannan, YIN Junyi, WANG Yan, QIN Pingwei, SHI Tongrui, LIU Yu. Identification and Physicochemical Properties of Antifungal Substances of Bacillus amylolyticus SSY2[J]. , 2018, 45(11): 3221-3228.

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Identification and Physicochemical Properties of Antifungal Substances of Bacillus amylolyticus SSY2

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