鸡血细胞药物代谢模型的建立与应用

doi:10.16431/j.cnki.1671-7236.2017.10.034

Abstract

Abstract:

The experiment was aimed to establish a drug metabolic model of the blood cells in chicken. The effects of three kinds of culture medium (L-15,M199 and RPMI1640) and different concentrations of fetal bovine serum (FBS) were compared to optimize the culture condition of blood cells,and the proper time of medication was determined after the cell vitality was measured by MTT assay. The content of ribavirin and its metabolites (TCONH₂ and RTCOOH) were detected after the ribavirin was dosed. The results showed that the cell viability was highest when the blood cells cultured in L-15 medium,the state of blood cells was better when 10% FBS was added to the medium. The best time for medication was when blood cells were cultured for 3 h. The content of ribavirin was decreased with the time of administration,the metabolites of ribavirin were increased quickly after half an hour, it changed slowly after 3 h. In conclusion,the metabolic model of blood cells in chicken was successfully established,and the blood cells cultured in vitro were better using L-15 medium supplemented with 10% FBS. The metabolic transformation function of blood cells in chicken was indicated by the medication test of ribavirin and it could be used to study the drug metabolism in vitro.

Key words: chicken blood cells; ribavirin; drug metabolism in vitro

CLC Number:

R969.1

ZHANG Xiao-qing, TANG Xiao-yan, YANG Xiao-ti, SHEN Xi-xi. The Establishment and Application of Metabolic Model of Blood Cells in Chicken[J]. , 2017, 44(10): 3070-3076.

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The Establishment and Application of Metabolic Model of Blood Cells in Chicken

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