屎肠球菌荚膜多糖的提取纯化及其多糖含量的测定

doi:10.16431/j.cnki.1671-7236.2017.05.008

Abstract

Abstract:

In order to extract and purify the capsular polysaccharide of Enterococcus faecium and measure contents of polysaccharide, high pressure crushing methods and enzyme decomposition methods (RNase A (25 μg/mL), DNase (0.5 mg/mL), lysozyme (0.25 mg/mL) and protease K (1.25 mg/mL)) were used to remove the nucleic acids and proteins to obtain crude capsular polysaccharide, and centrifugal ultrafiltration with 30 and 100 ku ultrafiltration centrifuge tubes and DEAE Sepharose Fast Flow column chromatography were also adopted to obtain the purified capsular polysaccharides of Enterococcus faecium. Then the content of polysaccharide was determined by the phenol-sulfuric acid method. The results showed the capsular polysaccharide content was 75.21%. The results revealed that capsular polysaccharide ofEnterococcus faecium was extracted successfully. The method of extracting capsular polysaccharide in Enterococcus faecium was established initially.

Key words: Enterococcus faecium; capsular polysaccharide; extraction and purification; phenol-sulfuric acid method

CLC Number:

S852.61

LIU Yong-an, LI Xue-rui, ZHOU Jian-hua, MA Li-na, LIU Yong-sheng. Extraction,Purification and Determination of Capsular Polysaccharide in Enterococcus faecium[J]. , 2017, 44(5): 1309-1314.

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Extraction,Purification and Determination of Capsular Polysaccharide in Enterococcus faecium

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