黑丝羽乌骨鸡精液DMA颗粒冷冻技术研究

doi:10.16431/j.cnki.1671-7236.2017.02.021

Abstract

Abstract:

The aim was to explore the effects of different kinds of dilution and thawing devices on the N,N-dimethylformamide (DMA) pellet frozen semen of Black Silkies. Firstly,the motility and fertility of the frozen semen thawed by different dilutions were compared;Then,the motility of the frozen semen was compared when the pellets were thawed using different tube and different number.Finally,the motility and fertility of the sperm thawed by three kinds of thawing devices (thermostat water bath,hotfunnel and hotplate ) were tested. The results showed that:①There was similar order of the sperm motility and fertility in the different dilution groups (LR > F > B > L),and there was significant difference among those groups (P < 0.05).②The motility was the best when the frozen semen was thawed with large thin-wall glass tube at 60℃.③The best temperature range of the 3 devices was different. The highest motility for thermostat water bath was 50 to 60℃ (0.51 to 0.59),and thermostat hotfunnel was 40 to 45℃ (0.42 to 0.46),while thermostat hotplate was 50 to 55℃ (0.61 to 0.63).There was no significant difference of the motility in the optimum temperature range for each device (P > 0.05).④The fertility of the different devices in their best thawing temperature was 26.91% (55℃,thermostat hotplate),23.08% (60℃, thermostat water bath), 20.93% (40℃, thermostat funnel),respectively,and there was no significant differences among those groups (P > 0.05).Therefore,the efficiency thawing condition for the Black Silkies frozen semen was the LR diluent,DMA cryoprotectant,pellet freezing,thawed in the thermostat hotplate at 54.9℃.

Key words: Black Silkies chicken; dilutent; DMA; pellets frozen semen; thawing device

CLC Number:

S831.2

HUANG Lin, SUN Ao, ZHU Li-jun, PENG Yun-chao, WANG Kun, JIA Xing-lin, YAN Hai-feng. Study on Technology of Black Silkies Chicken Semen Freezing by DMA Pellet[J]. , 2017, 44(2): 447-455.

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Study on Technology of Black Silkies Chicken Semen Freezing by DMA Pellet

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