紫玉米花色苷抑制牛传染性鼻气管炎病毒在牛肾细胞中增殖的研究

Study on Purple Corn Anthocyanin Inhibits Replication of Infectious Bovine Rhinotracheitis Virus on Bovine Kidney Cells

JIANG Liang¹^，2, HUANG Xiu-fen², LIU Shan-shan², YAN Min-xin², ZHAO Xiao-yan³, ZHANG Lin-bo¹, LI Yong-qing²

1.College of Animal Science and Technology, Jilin Agricultural University, Changchun 130118, China; 2.Institute of Animal Husbandry and Veterinary Medicine, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, China；3.Vegetable Research Center, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, China

Received:2014-02-20 Online:2014-08-20 Published:2014-08-22

Abstract

Abstract: To investigate the effect of purple corn anthocyanins (PCA) on inhibiting infectious bovine rhinotracheitis virus (IBRV) in vitro, the toxicity of PCA on madin-darby bovine kidney cells (MDBK) was measured by observation of cytopathic effect (CPE).Then the inhibition effects of PCA on replication of IBRV were investigated within safe concentrations by MTT assay. The results showed the maximum safe concentration of PCA on MDBK cell was 262.14 μg/mL, whereas PCA could apparently inhibit the replication of IBRV on MDBK cells in very low dose (7.8125 μg/mL). The median inhibitory concentration (TC₅₀ and IC₅₀) of PCA to MDBK and IBRV was 262.14 and 11.38 μg/mL，individually. On consequence, the treatment index （TI） was calculated as 23.04. These data demonstrated that PCA had the function of anti-IBRV, and suggested that purple corn could be as a feed material for prophylaxis and treatment of infectious bovine rhinotracheitis in dairy cows.

JIANG Liang, HUANG Xiu-fen, LIU Shan-shan, YAN Min-xin, ZHAO Xiao-yan, ZHANG Lin-bo, LI Yong-qing. Study on Purple Corn Anthocyanin Inhibits Replication of Infectious Bovine Rhinotracheitis Virus on Bovine Kidney Cells[J]. .

References

1 史海英，吕晓玲.紫玉米色素的稳定性研究［J］. 现代食品科技，2007，23(13)：8～10.
2 石建平, 孟日增, 马海利, 等. 牛传染性鼻气管炎病毒gB基因的截短表达与抗原反应性分析［J］. 中国兽医学报，2010，30：1313～1317.
3 刘钊，石福忠，杨占秋. 连花清瘟胶囊抗柯萨奇B4病毒作用的实验研究［J］. 中南民族大学学报(自然科学版)，2012，31(1)：20～25.
4 何健民，石立莹，李晓眠，等. 检测S632A对细胞毒性作用两种方法比较的实验研究［J］.重庆医学，2004，10：1566～1567.
5 张致平. 微生物药物学［M］. 北京: 化学工业出版社, 2003.
6 李双杰, 张宝林, 邓辉, 等. 苦瓜素对柯萨奇B3病毒RNA复制抑制作用的研究［J］. 上海中医药杂志，2001，35(5)：45～46.
7 杨振东.用响应面法优化紫玉米芯花色苷提取工艺参数［J］. 中国调味品，2009，34(11)：47～50.
8 赵晓燕，张超，马越，等.紫玉米花色苷对小鼠免疫功能的影响［J］.湖北农业科学，2010，49（8）：1933～1936.
9 徐琼，何宇乾，吴海燕. 牛传染性鼻气管炎基因工程疫苗研究进展［J］. 中国畜牧兽医，2012，39(11)：52～55.
10 高阳, 董雪, 康廷国, 等. 牛蒡苷元体外抗流感病毒活性［J］. 中草药，2002，33(8)：724～726.
11 Brzozowska A, Rychowski M, Andrea D, et al. Point mutations in BHV-1 Us3 gene abolish its ability to induce cytoskeletal changes in various cell types［J］. Veterinary Microbiology, 2010(143): 8～13.
12 De Martino L, Marfe G, Consalvo M I, et al. Antiapoptotic activity of bovine herpesvirus type-1 (BHV-1) UL14 protein［J］. Veterinary Microbiology, 2007，123（1~3）: 210～216.
13 Kang M K, Lim S S, Lee J Y, et al. Anthocyanin-rich purple corn extract inhibit diabetes-associated glomerular angiogenesis ［J］. PLoS One, 2013, 8(11): e79823.
14 Li C Y, Kim H W, Li H, et al. Antioxidative effect of purple corn extracts during storage of mayonnaise［J］. Food Chemistry, 2014, 152: 592～596.
15 Long N, Suzuki S, Sato S, et al. Purple corn color inhibition of prostate carcinogenesis by targeting cell growth pathways［J］. Cancer Science, 2013,104(3): 298～303.
16 Petrini S, Ramadori G, Corrad A, et al. Evaluation of safety and efficacy of DNA vaccines against bovine herpesvirus-1 (BoHV-1) in calves［J］. Comparative Immunology, Microbiology and Infectious Diseases, 2011, 34: 3～10.
17 Zhao X，Corrales M，Zhang C，et al.Composition and thermal stability of anthocyanins from Chinese purple corn (Zea mays L.) ［J］.Joumal of Asricultural and Food Chemistry，2008，56(22)：10761～10766.
18 Zhao X，Zhang C，Corrales M，et al.Composition，antimicrobial activity，and antiproliferative capacity of anthocyanin extracts of purple corn (Zea mays L.)from China［J］.European Food Research and Technology，2009，228(5)： 759～765.

Study on Purple Corn Anthocyanin Inhibits Replication of Infectious Bovine Rhinotracheitis Virus on Bovine Kidney Cells

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