新疆阿勒泰羊钙蛋白酶抑制蛋白基因多态性与嫩度的关联性分析

Association of Calpastatin Gene Polymorphisms with Meat Tenderness in Altay Sheep from Xinjiang

TIAN Jia¹, ZHOU Wei-dong², MA Xiao-yan¹, ZHENG Wen-xin², GAO Wei-ming², DI La-re·SHA Ta-er², ZUO Xiao-jia², SHI Shuai², ZHANG Rong-yin², LIU Wu-jun¹

(1.College of Animal Science, Xinjiang Agricultural University, Urumqi 830052,China；2.Xinjiang Breeding Sheep and Wool Cashmere Quality and Safety Supervisory and Inspection Center，Urumqi 830001,China)

Revised:2014-03-11 Online:2014-06-20 Published:2014-07-24

Abstract

Abstract: In this study, Altay sheep was used for investigating the association between sheep tenderness and single nucleotide polymorphism markers on the calpastatin （CAST） gene. Its genetic polymorphism was detected using SSCP and DNA sequencing technology in exon 1, exon 2 and exon 21. However, only three genotypes AA, AB and BB were detected in exon 21, the sequencing result showed that the mutation of A into G, association analysis indicated that the mutation in exon 21 affected the meat tenderness markedly of Altay sheep (P＜0.05). In summary, AA genotype might have certain improvement effect on meat tenderness.

Key words:

CAST gene; Altay sheep; tenderness

TIAN Jia, ZHOU Wei-dong, MA Xiao-yan, ZHENG Wen-xin2, GAO Wei-ming, DI La-re?SHA Ta-er, ZUO Xiao-jia, SHI Shuai, ZHANG Rong-yin, LIU Wu-jun. Association of Calpastatin Gene Polymorphisms with Meat Tenderness in Altay Sheep from Xinjiang[J]. .

References

1 邓桂馨，张银花. CAST基因分子多态性与肉品质性状相关性的研究［J］. 北京农学院学报，2007，3（22）：21～24.
2 孙立彬, 孟和, 李蜻, 等. 梅山猪等5个群体中钙蛋白酶抑制蛋白基因的多态性及其与肉质和背膘厚的关系［J］. 农业生物技术学报，2006，14（2）：156～161.
3 薛慧良，徐来祥. 猪CAST基因的单核苷酸多态性及其对肉质性状的效应［J］. 生态学报，2008，28（6）：2937～2944.
4 邱大伟. 西南地区本地黄牛及其杂交牛CAST、H-FABP、MyoD1基因部分编码区多态性［D］. 杨凌: 西北农林科技大学，2009a.
5 邱大伟, 赵金红, 黄勇富. 重庆山地黄牛及其杂交牛CAST基因部分片段PCR-SSCP分析［J］. 安徽农业科学，2009b，37（13）：5891～5893.
6 张瑜，王昕陟. 钙蛋白酶系统对猪肉嫩度影响的研究进展［J］. 中国畜牧兽医，2013, 40（10）：161～164.
7 赵先萍，黎威，王彦玲，等. 猪钙蛋白酶抑制蛋白基因外显子9多态性检测及其对山西白猪背膘厚的影响［J］.中国畜牧兽医，2013,40（5）：161～165.
8 萨姆布鲁克J，拉塞尔D W. 黄培堂等译.分子克隆实验指南［M］. 第3版. 北京：科学出版社，2002.
9 Delgado E F, Geesink G H, Marchello J A, et al. The calpainsystem in three muscles of normal and callipyge sheep[J］. J Anim Sci, 2001, 79(2):398～412.
10 Goll D E, Thompson V F, Taylor R G, et al. Role of calpain system in muscle growth［J］. Biochimie, 1992, 74(3):225～237.
11 Kocwin-Podsiadla M, Kuryl J, Krzecio E, et al. The interaction between calpastatin and RYR1 genes for some pork quality traits［J］. Meat Sci,2003,65（2）:731～735.
12 Koohmaraie M. Biochemical factors regulating the tougheing and tenderizationg processes if meat［J］.Meat Sci,1996,43（S1）:193～201.
13 Mellgren R L, Murachi T. Intracellular calcium-de-pendent proteolysis［M］.Boca Raton,FL:CRC,1990.
14 Palmer B R, Roberts N, Hickford J G, et al. Rapid communication：PCR-RFLP for MspⅠ and NcoⅠ in the ovine calpastatin gene［J］. Anim Sci,1998,76:1499～1500.
15 Zhou H, Hickford J G H, Gong H, et al. Polymorphism of the ovine calpastatin gene［J］. Molecular and Cellular Probes, 2007,21(3):242～244.
16 Zhou H, Hiekford J G. Allelie variation of the bovine calpastatin (CAST) gene［J］. Molecular and Cellular Probes, 2008a, 22（2）:129～130.
17 Zhou H, Byun S O, Frampton C M, et al. Lack of association between CAST SNPs and meat tenderness in sheep［J］. Animal Genetics,2008b,39(3):331～332.

Association of Calpastatin Gene Polymorphisms with Meat Tenderness in Altay Sheep from Xinjiang

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