不同源鼠伤寒沙门氏菌耐药性质粒不相容性分群的初步研究

Preliminary Study on Incompatibility Plasmids of 101 Strains ofSalmonella typhimurium by PCR-based Replicon Typing

LI Jing-yi,CUI Ke-qi,FENG Sai-xiang,HE Xian-hui,LIAO Ming,XU Cheng-gang

(Key Laboratory of Veterinary Vaccine Innovation, Ministry of Agriculture, College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China)

Received:2013-09-06 Online:2014-03-20 Published:2014-05-15

Abstract

Abstract: The incompatibility plasmids of 101 strains Salmonella typhimurium were detected by PCR-based replicon typing. Eighteen pairs of primers were designed to perform 5 triplex-PCRs and 3 simplex-PCRs, recognizing FIA, FIB, FIC, HI1, HI2, I1, L/M, N, P, W, T, A/C, K, B/O, X, Y, F and FII. 94 strains (93.1%) Salmonella typhimurium carried a total of 10 Inc groups. The results suggested that the method was applicable for a large number of strains to trace the diffusion of specific multi-drug resistance plasmids in different environments.

Key words: Salmonella typhimurium; plasmid typing; incompatibility plasmid

LI Jing-yi,CUI Ke-qi,FENG Sai-xiang,HE Xian-hui,LIAO Ming,XU Cheng-gang. Preliminary Study on Incompatibility Plasmids of 101 Strains ofSalmonella typhimurium by PCR-based Replicon Typing[J]. .

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Preliminary Study on Incompatibility Plasmids of 101 Strains ofSalmonella typhimurium by PCR-based Replicon Typing

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