Abstract: Excessive amount of antibiotic residues in milk，would seriously endanger the health of consumers.Currently，the maturity and development of high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) technology which has became a powerful tool to solve above problem. This technology combined separation，qualitative analysis and quantitative analysis，which has also been fast analysis, strong selectivity，high sensitivity and good repeatability，and could detect multiple medicines in one sample simultaneously.This paper summarized the recent research progress on determination of antibiotic residues in milk by HPLC-MS/MS technology，and elaborated the pretreatment method，chromatography and mass spectrometry conditions and determination results of HPLC-MS/MS technology.

Key words: antibiotic residues; high performance liquid chromatography-tandem mass spectrometry