犬布鲁氏菌菌壳疫苗的制备

Preparation of Brucella canis Ghosts Vaccine

QIAN Jing^1,2, YIN Qiu-dan^2,3, BU Zhao-yang^1,2, CHEN Si², WANG Xing-long^2,3, WANG Xiu-ran², LANG Xu-long^2,3, WANG Xiao-xu²

(1. College of Animal Science and Technology, Jilin Agricultural University, Changchun 130118, China; 2.Institute of Military Veterinary, Academy of Military Medical Sciences, Changchun 130122, China;3. Key Laboratory of Jilin Province for Zoonosis Prevention and Control, Changchun 130122, China)

Online:2014-01-20 Published:2014-02-27

Abstract

Abstract: Bacterial ghost technology is a novel method for preparing the inactivated vaccine. This nondenaturing inactivated method keeps many antigenic determinants which serves as a candidate vaccine to prevent pathogenic bacteria. This experiment took lysis E gene from phage PhiX174 DNA by PCR and inserted E gene into pBV220 vector, then cloned protein lysis component (PLC) from expression vector pBV220+E which we had constructed before, this PLC contained repressor protein cI857, lysis E gene and terminator sequence rrnbT1T2, as well inserted PLC into the broad-host shuttle vector pBBR1MCS-2. Finally, the broad-host lysis plasmid pBBR+PLC was electrical transformed into Brucella canis RM6/66. The results showed that the broad-host lysis plasmid had highly killing effect for Brucella canis RM6/66 after 42 ℃ induction and its lysis rate was 100%, meanwhile Brucella canis RM6/66 ghosts were successfully generated. This study used bacterial ghost technology to prepare the Brucella canis ghost, which played an important role in the prevention of canine brucellosis, simultaneously provided a new strategy for human and animals’ vaccine against brucellosis.

Key words: Brucella canis; bacterial ghost; vaccine

QIAN Jing，YIN Qiu-dan，BU Zhao-yang，CHEN Si，WANG Xing-long，WANG Xiu-ran，LANG Xu-long，WANG Xiao-xu. Preparation of Brucella canis Ghosts Vaccine[J]. .

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