人α-乳清蛋白转基因克隆牛外源基因拷贝数的分析

Abstract

Abstract: To explore the changes of the exogenous gene copy number in transgenic cloned animals during subculture, we detected the copy number of human alpha-lactalbumin gene in five transgenic cloned cattles, including a parental cattle(K060923) and four offspring cattles(172,081,189 and Longwa), with Real-time fluorescent quantitative PCR. Among the five cows, K060923 was parental and the rest are offspring. The copy number of K060923 was 1.145, and that of the four offspring (172, 081, 189 and Longwa) was 0.998, 1.107, 0.891 and 0.842, respectively. The obtained results indicated that the copy numbers of all the five cows were about one. The results showed that exogenous gene was integrated into the host genome as a single copy and was stably inherited.

Key words: human alpha-lactalbumin; transgenic cloned cattle; Real-time fluorescent quantitative PCR; copy numbe

CLC Number:

WANG Jie-wen, WANG Jing-jiao, ZHAO Shu-jun, LI Ning, LI Shi-jie, ZHANG Cui. Detecting Copy Number of Foreign Gene in Human Alpha-lactalbumin Transgenic Cloned Cattle[J]. , 2013, 40(9): 183-187.

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Detecting Copy Number of Foreign Gene in Human Alpha-lactalbumin Transgenic Cloned Cattle

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