鱼源维氏气单胞菌的耐药性研究

Abstract

Abstract: To detect the drug resistance genotype and phenotype produced by Aeromonas veronii isolated from different fish.PCR method was used to detect the aminoglycoside antibiotics resistance genes (aac (3)-Ⅱa, aac (6')-Ⅰb, ant (3")-Ⅰa, aph (3')-Ⅱa), sulfonamides resistance genes (Sul1, Sul2, Sul3) and tetracyclines resistance genes (tetA, tetC, tetM) in isolates. The drug susceptibility to seven Aeromonas veronii strains toward 22 kinds of antibiotics were performed with Kirby-Bauer disc diffusion method. The results showed that the detected resistance genes were aac (3)-Ⅱa (71.4%), aac (6')-Ⅰb (85.7%), Sul2 (85.7%) and tetA (28.5%),while the other resistant genes of ant (3")-Ⅰa, aph (3')-Ⅱa, Sul1, Sul3, tetC and tetM were not checked out. The seven Aeromonas veronii isolates were sensitive to Fosfomycin (100%), Polymyxin B (100%), Furazolidone (85.7%) and Ofloxacin (71.4%). They were resistant to Ampicillin (100%), Acetylspiramycin (100%), Trimethoprim/Sulfamethoxazole (85.7%), Sulfisoxazole (85.7%) and Tetracycline (85.7%). Drug resistance genotype had some correlation with the drug resistance phenotype.

Key words: Aeromonas veronii; drug resistance genotype; drug resistance phenotype

CLC Number:

S852.61

WANG Hai-juan, WANG Li, GOU Xiao-lan, LONG Zhong-ming, ZHAO Jing. Study on Drug Resistance in Aeromonas veronii Isolated from Different Fish[J]. , 2013, 40(8): 159-164.

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Study on Drug Resistance in Aeromonas veronii Isolated from Different Fish

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