弯曲杆菌中具有高抑菌活性乳酸菌的筛选

Abstract

Abstract: Using probiotics replaced antibiotic for the treatment and prevention of animal in the future, and lactic acid bacteria (LAB) were the application of probiotics in the main component. Test was aimed to screen LAB which had high bacteriostatic activity to Campylobacter and tolerance animal gastrointestinal environment. Poultry excrement were acquired from Guangdong different areas, the isolated LAB were dealed with biochemical identification, PCR identification and 16S rDNA sequencing. Meanwhile, we tried to study the antibacterial ability of them against Campylobacter jejuni isolated from chicken, duck and Campylobacter coli from goose, then we further examed the bearing capability of the LAB under acid, bile salts and trypsin. The results showed that Lactobacillus saliva R3 had high bacteriostatic activity and tolerance ability to different birds source Campylobacter. So, Lactobacillus saliva R3 would be one of candidate strains that used as microecologics to treat poultry Campylobacter disease.

Key words: Campylobacter; lactic acid bacteria; bacteriostatic activity; tolerance

CLC Number:

S852.61⁺3

RAO Lei, HUANG Wu, HUANG Yong-xing, GAO Rui-juan, LUO Kai-jian. Screening of Lactobacillus with Highly Antagonistic Activity against Campylobacter[J]. , 2013, 40(6): 129-134.

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Screening of Lactobacillus with Highly Antagonistic Activity against Campylobacter

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