卵泡抑素基因过表达对其通路上相关基因表达的影响

Abstract

Abstract: To investigate the effect of follistatin (FST) gene over-expression on related genes in its pathway, this study cloned the FST gene of Min pig with PCR method, constrcuted eukaryotic expression vector pcDNA3.1(+)-Dsig-FST, identified fibroblast cells transfected with the recombinant plasmid, and detected the change of related genes in its pathway with Real-time PCR method. The results showed that FST gene of Min pig was successfully cloned and over-expressed significantly in the porcine fetal fibroblast cell, and then lead to ActRⅡB and BMP4 gene decline significantly (P<0.05),Smad4 and Myostatin gene had downward trend, ActRⅡA gene had the upward trend. In summary, FST gene changed in its expression had the different effect of degrees inhibition or promotion on its related genes.

Key words: follistatin (FST); clone; eukaryotic expression; porcine fetal fibroblast; pathway gene

CLC Number:

Q786

SUN Ya-meng, ZHANG Dong-jie, ZHANG Xu, WANG Liang, XIE Yu, LIU Di. Effect of FST Gene Over-expression on Expression of Related Genes in its Pathway[J]. , 2013, (5): 35-39.

References

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Effect of FST Gene Over-expression on Expression of Related Genes in its Pathway

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