地黄归芩胶囊对特应性皮炎的治疗效果及作用机制研究

doi:10.16431/j.cnki.1671-7236.2025.05.041

Abstract

Abstract: 【Objective】 The objective of this experiment was to investigate the therapeutic effect and mechanism of Dihuang Guiqin capsules on atopic dermatitis induced by dinitrochlorobenzene solution in rats,and provide theoretical basis for the research and development of new veterinary drugs for pets.【Method】 The atopic dermatitis model was replicated by applying 2% dinitrochlorobenzene solution on the back of rats.After successful modeling,40 rats with atopic dermatitis were randomly divided into 4 groups:Model control group,Dihuang Guiqin capsules low (0.1 g/kg),medium (0.2 g/kg) and high (0.4 g/kg) dose groups,with 10 rats in each group.10 healthy rats were selected as blank control group.Rats in each dose group were given Dihuang Guiqin capsules by intragastric administration according to the corresponding dose.Rats in model control and blank control groups were given intragastric drinking water,0.2 mL/rat each time,once a day,for 14 consecutive days.The skin lesions of rats were evaluated according to SCORAD.Scratching times of rats were recorded to evaluate the pruritus of rats.The pathological changes of the skin were observed by HE staining,and the histopathological score was performed by Baker score.The number of mast cells was detected by toluidine blue staining.Western blotting was used to detect the expression of interleukin-31 (IL-31) protein in skin tissues.【Result】 Compared with blank control group,the skin lesion score of rats in model control group was significantly increased (P<0.05),a large number of inflammatory cells infiltrated,and the Baker score was significantly increased (P<0.05).Compared with model control group,the skin lesion score and scratching times of rats in each dose group of Dihuang Guiqin capsules were significantly decreased (P<0.05).The Baker score and the number of mast cells in medium and high dose groups of Dihuang Guiqin capsules were significantly decreased (P<0.05),and the expression of IL-31 protein in skin tissue was significantly decreased (P<0.05).【Conclusion】 Dihuang Guiqin capsules could effectively relieve pruritus in rats with atopic dermatitis,and its mechanism related to repairing skin barrier,relieving inflammatory cell infiltration,reducing the number of mast cells and down-regulating IL-31 protein expression.

Key words: Dihuang Guiqin capsules; atopic dermatitis; mast cells; IL-31

CLC Number:

S859.3

QIN Junjie, WU Chao, HUANG Xueli, ZHOU Changxi, HOU Xiaojiao, WANG Xiumin, LI Jianxi. Study on the Therapeutic Effect and Mechanism of Dihuang Guiqin Capsules on Atopic Dermatitis[J]. China Animal Husbandry and Veterinary Medicine, 2025, 52(5): 2392-2399.

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Study on the Therapeutic Effect and Mechanism of Dihuang Guiqin Capsules on Atopic Dermatitis

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