短小乳杆菌内蒙古分离株S-层蛋白的普查、鉴定和基因分析

Detection,Identification and Genetic Analysis of S-Layer Protein of Lactobacillus brevis Isolated from Inner Mongolia

LAN Li^1,2, WEI Jian-min², WANG Yan-xia¹, WANG Min¹, MAN Da¹, Geriletu¹

1. Ministry of Education Key Laboratory of Dairy Biotechnology and Engineering, Inner Mongolia Agricultural University, Hohhot 010018,China;
2. College of Life Sciences,Inner Mongolia Agricultural University,Hohhot 010018,China

Received:2012-03-12 Online:2012-08-20 Published:2012-08-24

Abstract

Abstract: The surface layers protein (SLP) is a major composition of the bacterial outermost envelope with highly ordered ultrastructure. The Lactobacillus brevis which isolated from Mongolian traditional dairy products were used as materials in this study. The S-layer proteins from several Lactobacillus were detected by SDS-PAGE and slp gene was cloned by PCR and then analyzed the slp gene sequence of Lactobacillus breris. The results showed that the protein sample of Lactobacillus breris showed target band about 44 to 55 ku which had the same size range of SLP that had been reported,and slp gene about 1300 bp was amplified by PCR method. The DNAStar software found that the protein had recently kinship and genetic distance with L.gallinarum strain (GenBank:AY597266.1) and was similar to other Lactobacillus spp. The theoretical size of S-layer protein was about 47 ku released by the Genetyx software and had significant feature of outer membrane proteins. This layed foundation for genetic modification and other research work.

Key words: Lactobacillus brevis; S-layer protein; slp gene; cloning and sequence analysis

CLC Number:

Q781

LAN Li, WEI Jian-min, WANG Yan-xia, WANG Min, MAN Da, Geriletu. Detection,Identification and Genetic Analysis of S-Layer Protein of Lactobacillus brevis Isolated from Inner Mongolia[J]. .

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Detection,Identification and Genetic Analysis of S-Layer Protein of Lactobacillus brevis Isolated from Inner Mongolia

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