马疱疹病毒1型感染马疾病模型的建立及评价

doi:10.16431/j.cnki.1671-7236.2021.06.033

Abstract

Abstract: This study was aimed to establish the artificial disease model of Equine herpesvirus type 1 (EHV-1),and confirm the half infective dose (ID₅₀) of equine infected with EHV-1 and the criteria for determining the incidence of infection,and lay the foundation for prevention of the disease and therapeutic drugs.Taking EHV-1 XJ2015 strain isolated from the aborted fetuses of a racetrack in Yili area of Xinjiang as the research object,4 groups of different virus dose challenge and control group were set up,and 5 mL of virus was sprayed into each horse’s nasal cavity.The clinical symptoms and pathogenesis of the horses were observed every day.14 days later,the pathological changes of tissues and organs were observed and the nasal detoxification and virus distribution were detected by Real-time PCR method.The results showed that the ID₅₀ of EHV-1 XJ2015 strain was 10^-6.67/5 mL for the horse,and the virus content was 10^4.33 TCID₅₀/mL.Compared with control group,the clinical scores of horses in 1×10⁶ and 1×10⁵ TCID₅₀/mL challenge groups increased significantly,high body temperature (up to 39.5 ℃,generally lasting for 2-6 days),loss of appetite,fluid nasal and mandibular lymphadenopathy.And the test horses in 1×10⁶ and 1×10⁵ TCID₅₀/mL challenge groups showed different levels of detoxification,a large number of viruses could be detected in the lung and brain tissues compared with control group (P<0.01;P<0.05).Histopathological examination revealed non-suppurative encephalitis and neuronal edema in the brain tissue of the horse,interstitial pneumonia,neutrophils,inflammatory cell infiltration,hemorrhage,and alveolar septal thickening occur in lung tissue.The above results showed that EHV-1 XJ2015 strain had strong pathogenicity to horses,the clinical symptoms of diseased horses were typical,the virus was mainly excreted with nasal fluid,and enriched in lung and brain tissue,and the criteria of EHV-1 infection in horses could be determined through the above indexes.The disease model of EHV-1 infected animals was successfully established in this experiment.

Key words: Equine herpesvirus type 1 (EHV-1); model; judgement standard

CLC Number:

S852.65^＋2

CHE Chuanzhong, BAO Zilei, HU Yue, ZHENG Xuegong, LIU Jianhua, RAN Duoliang. Establishment and Evaluation of Equine Herpesvirus Type 1 Infection Equine Disease Model[J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(6): 2196-2203.

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Establishment and Evaluation of Equine Herpesvirus Type 1 Infection Equine Disease Model

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