添加不同水平单宁酸对绵羊体外发酵参数和甲烷产量的影响

doi:10.16431/j.cnki.1671-7236.2020.05.016

Abstract

Abstract: In this experiment,concentrate,hay and tannic acid were used as fermentation substrates to investigate the effects of different levels of tannic acid on in vitro fermentation parameters,methane production,the degradation rate of dry matter and crude protein in sheep,in order to screen out the optimal concentration of tannic acid,and as a theoretical basis for untilization of tannic acid-rich roughage resources apply to dietary formulation and reducing methane emission in the process of ruminate farming.Batch culture in vitro was used for this study,the fermentation substrate was concentrate,hay and tannic acid,and the ratio of concentrate to roughage was 3:7.The supplertation level of tannic acid was 0,0.5%,1.0%,1.5%,2.0% and 3.0%,respectively,the fermentation time was set at 3,6,12 and 24 h,three replicates were set for each treatment.The pH,the concentration of ammonia nitrogen (NH₃-N) and volatile fatty acid (VFA),total gas production,methane production and the degradation rate of dry matter and crude protein were determined.The results showed that pH in 1.0% and 2.0% tannic acid groups was significantly higher than that in control group at 3 h (P<0.05),and there was no significant difference between all groups at other time points (P>0.05).The concentration of NH₃-N in 1.0%,2.0% and 3.0% tannic acid groups was significantly lower than that in control group at 3 h (P<0.05),more over the concentration of NH₃-H in each treatment group was significantly lower than that in control group at 6 and 24 h (P<0.05).The concentration of acetic acid,propionic acid,butyric acid and total VFA in 2.0% tannic acid group was significantly lower than that in control group at 6 h (P<0.05),the concentration of propionic acid,butyric acid and total VFA in 1.0%,1.5% and 3.0% tannic acid groups were significantly lower than that in control group at 24 h (P<0.05),the average concentration of propionic acid,butyric acid and total VFA in each tannic acid adding group was significantly lower than that in control group at 3,6,12 and 24 h(P<0.05).There was no significant difference in total gas production among each group at 12 and 24 h (P>0.05).The methane production in 2.0% tannic acid group was significantly lower than that in control,0.5% and 1.0% tannic acid groups at 12 h (P<0.05).The methane production in 2.0% and 3.0% tannic acid groups were significantly lower than that in control group at 24 h (P<0.05).In conclusion,the addition of tannic acid could significantly reduce the concentration of NH₃-N in vitro fermentation and inhibit the production of VFA,the addition of 1.5% and 2.0% tannic acid could significantly reduce the rumen methane production in vitro,the degradation rate of crude protein in the treatment group with tannic acid was significantly lower than that in control group.

Key words: tannic acid; methane; rumen fermentation; sheep; in vitro

CLC Number:

S816.7

WANG Jingyao, XIE Yongfang, LI Li, LI Zinan, LI Dabiao. Effects of Adding Different Levels of Tannic Acid on Rumen Fermentation Parameters and Methane Production of Sheep in Vitro Method[J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(5): 1428-1435.

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Effects of Adding Different Levels of Tannic Acid on Rumen Fermentation Parameters and Methane Production of Sheep in Vitro Method

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