China Animal Husbandry and Veterinary Medicine ›› 2019, Vol. 46 ›› Issue (9): 2787-2794.doi: 10.16431/j.cnki.1671-7236.2019.09.035

• Basic Veterinary Medicine • Previous Articles     Next Articles

Research Pregress on New Detection Methods for the Pathogens of Swine Bacterial Infectious Diseases

CHEN Dishi1, CHEN Bin1, REN Yupeng2, SHAO Jing1, ZHANG Yi1, WANG Zezhou1   

  1. 1. Center for Animal Disease Control and Prevention of Sichuan Province, Chengdu 610041, China;
    2. College of Life Science and Technology, Southwest University for Nationalities, Chengdu 610041, China
  • Revised:2019-02-22 Online:2019-09-20 Published:2019-09-21

Abstract: The swine bacterial infectious diseases in China whose outbreak situation has change gradually in recent years is a serious threat to pig-breeding industry and public health security.The rapid and accurate detection of pathogens is a key point to prevent and control swine diseases.Traditional techniques,such as isolation and cultivation of pathogens,conventional PCR and early immunological assays has been constantly changed and evolved to be more accurate and efficient with the development of biotechnology and the intersections of different disciplines.This article highlighted several new detection technologies:Real-time quantitiative PCR,loop-mediated isothermal amplification,sandwich DNA hybridization assay which based on nucleic acid molecular biology;Immune colloidal gold technique,time resolved fluorescence immunoassay,immunomagnetic bead separation which base on immunology;Mass spectrograph detection of bacteria which base on protein molecular biology and The POCT technology appropriated to on-site detection.The advantages and disadvantages of these new technologies wre analyzed,which would be helpful to popularize this detection in different institutions and research departments to select in order to make them play a more important role in the detection and surveillance of swine bacterial infectious diseases.

Key words: swine bacterial infectious diseases; detection of pathogens; diagnostic method

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