不同血清型牛源巴氏杆菌多重PCR检测方法的建立

›› 2010, Vol. 37 ›› Issue (8): 175-176.

• 疾病防治 • Previous Articles Next Articles

Multiplex PCR for Identification of Bovine Pasteurella mutocida from Different Capsular Serotype

DUAN Xin-hua, LI Jian-jun，YANG Xue-yun, WANG Deng-feng, WANG Zhi-cai

(Institute of Veterinary Medicine, Xinjiang Academy of Animal Science, Urumqi 830000，China)

Received:1900-01-01 Revised:1900-01-01 Online:2010-08-20 Published:2010-08-20

Abstract

Abstract: Pasteurella mutocida is an important pathogen of bovine hemorrhagic septicemia and the pathogenic serotype are capsular serotype A, B and E. Three specific primers to capsular serotype A, B and E of Pasteurella mutocida were selected and synthesized and multiplex PCR method used for identification of different capsular serotype of Pasteurella mutocida was developed. Target genes could be amplified with 2.5 ng DNA template. The results showed that the method was specific, high sensitive in identification and classification of Pasteurella mutocida.

Key words: Pasteurella mutocida; bovine hemorrhagic septicemia; multiplex PCR

CLC Number:

S858.23

DUAN Xin-hua;LI Jian-jun;YANG Xue-yun;WANG Deng-feng;WANG Zhi-cai. Multiplex PCR for Identification of Bovine Pasteurella mutocida from Different Capsular Serotype[J]. , 2010, 37(8): 175-176.

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Multiplex PCR for Identification of Bovine Pasteurella mutocida from Different Capsular Serotype

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