Abstract: Using stimulated with ConA peripheral blood lymphocyte and a set of specific primers,the Interleukin-15 gene of swine was amplified by PCR.The primers were designed based on the published sequences of the swine IL-15 gene．The 489bp amplified DNA fragment was cloned into pMD18-T vetor．The results showed that the sequence identities were 99 between amplified IL15 gene and that from GenBank. Bioinformatics analysis indicated some Codon preference ,Phylogenetic tree analysis indicated that the have evolutionary relationship with mammalian IL-15,however showed distinct difference with chicken IL-15. There are distinct resemblance between pig IL-15 and human IL-15 by structural domain prediction analysis .

Key words: rongchang pig; pig IL-15; cloning; bioinformatics analysis