Abstract: GFP to the optical bleaching, the oxidant, the reducing agent, the acid, the alkali and all that many chemical reagent has the greatly strengthened stability. First, from includes GFP in the backwoods coli to withdraw the material particle, and causes its purification using the rubber recycling reagent box. At the same time, makes using the calcium chloride law project fungus DH_5a the feeling condition cell. Then will purify after the material particle to change over to already in the feeling condition cell which the system will arrange, will cause project fungus DH_5ato transform has the anti-ammonia animal pen penicillin the backwoods coli, and the vaccination in on the ammonification animal pen penicillin culture medium, willcause its growth.

Key words: GFP; engineering E coli DH_5a; plasmid pGEM