Abstract: 193 Guangxi Xinlin and Fuzhong buffalo blood samples were collected in this study. Polyacrylamide gel electrophoresis was employed to exam the serum transferring (Tf),serum albumin (Alb),and amylase (Amy). The genotype,frequency of genotype,and genetic variation coefficient were analyzed. The results showed that Tf^A and Tf^D alleles were found in Xilin and Fuzhong buffalo,the gene frequency of Tf^D＞Tf^A. Tf^D was dominant genes and DD was dominant genotype in two buffaloes. There were Alb^A and Alb^X alleles in two buffaloes and the gene frequency of Alb^X＞Alb^A. Meanwhile,Xilin buffalo had a higher XX and lower AX genotypes comparing with Fuzhong buffalo,respectively. There were six genotypes of AA,AB,BB,BC,AC,and CC which were controlled by three alleles of Amy^A,Amy^B,and Amy^C,respectively. Amy^A and AC were a dominant gene and genotype in Amy Ⅰ and Amy Ⅱ,respectively. Three proteins were not sex specific and the variants were a little.

Key words: Xilin buffalo; Fuzhong buffalo; serum proteins; polymorphisms