长春地区梅花鹿毕氏肠微孢子虫分子流行病学调查

doi:10.16431/j.cnki.1671-7236.2021.01.032

Abstract

Abstract: In order to investigate the infection and distribution of genotypes of Enterocytozoon bieneusi in Changchun region,and explore the nature of its transmission between humans and animals.A total of 538 feces samples were collected from 8 farms in Changchun region from December 2018 to December 2019 for epidemiological investigated and genotype analysis.After PCR amplification,electrophoresis identification,sequencing analysis,sequence splicing comparison,and phylogenetic analysis,the following results were obtained:The prevalence rate of Enterocytozoon bieneusi in Sika deer in Changchun region was 17.84%(96/538),and the prevalence in different farms was 0 to 47.96%.There were 14 genotypes of Enterocytozoon bieneusi was found in Sika deer in Changchun area(BEB6,EbpC,Ⅰ,JLD-Ⅲ,JLD-Ⅸ,JLD-ⅩⅤ,JLD-ⅩⅥ,JLD-ⅩⅦ,JLD-ⅩⅧ,JLD-ⅩⅨ,JLD-ⅩⅩ,JLD-ⅩⅪ,JLD-ⅩⅫ and JLD-ⅩⅫⅠ).Moreover,BEB6 was the dominant genotype.The multilocus sequence typing (MLST) method was used to analyze the polymorphism and population genetic structure of Enterocytozoon bieneusi positive samples by selecting gene loci MS1,MS3,MS4,and MS7.The results showed that 16 positive isolates of 96 positive samples were successfully amplified at two or more loci simultaneously,forming 10 multilocus genotypes (MLGs).At the same time,there were different subtypes between the same genotype.This result indicated that there was a rich genetic diversity of Enterocytozoon bieneusi of Sika deer in Changchun.In this study,molecular biological methods were used to analyze the epidemic situation and genotypes of Enterocytozoon bieneusi of Sika deer in Changchun area.The results not only provided a scientific basis for the prevention and control of Enterocytozoon bieneusi in Sika deer,but also provided basic data for the prevention and control of the spread of Enterocytozoon bieneusi between humans and animals,which was of great significance to public health and health.

Key words: Enterocytozoon bieneusi; molecular epidemiological investigation; genotype analysis

CLC Number:

S855.9

LI Jiandong, CUI Kai, DONG Huansheng, MA Zefang. Molecular Epidemiological Investigation of Enterocytozoon bieneusi in Sika Deer in Changchun Region[J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(1): 287-295.

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Molecular Epidemiological Investigation of Enterocytozoon bieneusi in Sika Deer in Changchun Region

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