基于LC-MS技术的代谢组学方法研究吡虫啉对工蜂代谢的影响

doi:10.16431/j.cnki.1671-7236.2019.08.004

Abstract

Abstract:

To identify the impact of sub-lethal dose of imidacloprid on honeybee metabolism during growth stage, the metabonomics analysis was conducted for one-day old worker bee based on liquid chromatography-mass spectrometry (LC-MS).One-day old Apis mellifera ligustica were fed with 0.00001 μg/μL sub-lethal dose of imidacloprid until defecation by the artificial indoor feeding technique.The eclosion rate, body weight at birth, out of the hive time, the activities of SOD, CAT and POD in bee hemolymph were analyzed respectively, and synchronously, differential metabolites were screened out through pattern recognition analysis by means of LC-MS.The results indicated that the out of hive time was significantly prolonged (P<0.05) and the activities of SOD and POD in bee hemolymph were significantly increased (P<0.05) with the treatment of sub-lethal dose of imidacloprid.Principal component analysis (PCA) and supervised partial leastsquares-discriminant analysis (PLS-DA) showed that the experimental group and the control group were significantly separated, the 18 different metabolites were identified.The results could provide theoretical guidance for the further research on the metabolic rules of bee metabolin under imidacloprid stress and elucidation of the bee detoxification mechanism.

Key words: imidacloprid; honeybee; metabonomics; metabolites analysis

CLC Number:

ZHUANG Mingliang, LI Zhiyong, WANG Jinzhou, LIU Nannan, WANG Zhi. Metabolomic Analysis Imidacloprid Effect of Honeybee Worker Based on Liquid Chromatograph Mass Spectrometer[J]. , 2019, 46(8): 2220-2227.

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Metabolomic Analysis Imidacloprid Effect of Honeybee Worker Based on Liquid Chromatograph Mass Spectrometer

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