›› 2019, Vol. 46 ›› Issue (5): 1551-1557.doi: 10.16431/j.cnki.1671-7236.2019.05.035

• Clinical Veterinary Medicine • Previous Articles     Next Articles

Diagnosis and Identification of a Mixed Infection of Escherichia coli and Eperythrozoon in Raccoon Dog

LUAN Mingming1,2, WANG Xiaoxu2, JIA Yun3, ZHAO Jiaping2, ZHANG Linbo1, XU Chao2, HAN Peng1   

  1. 1. College of Life Science, Jilin Agricultural University, Changchun 130118, China;
    2. State Key Laboratory of Special Economic Animal Molecular Biology, Institute of Special Animal and Plant Sciences, Chinese Academy of Agricultural Sciences, Changchun 130112, China;
    3. Liaoning Entry Exit Inspection and Quarantine Bureau, Dalian 116001, China
  • Received:2018-11-20 Online:2019-05-20 Published:2019-05-20

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Abstract:

To diagnose the pathogen of a diseased racoon dog in a Jilin farm,in this experiment,bacterial culture,staining microscopy,biochemical identification,molecular biology identification and blood smear microscopy were used to isolate and identify the pathogens of the collected lungs,liver,spleen,kidney,intestines and blood.And the isolated pathogens were tested for pathogenicity and susceptibility test.The results showed that a dominant strain was isolated,which was presented as a rod-shaped Gram-negative bacilli with obtuse ends at the optical microscope.The results of biochemical tests showed that it could decompose glucose-lactose,mannitol,maltose and sucrose to produce acid gas,which was positive for M-R test,sputum test and starch hydrolysis test.The 16S rRNA sequence had 99% homology with the known E.coli sequence registered in the NCBI database.The animal lethality test results showed that the strain was pathogenic to mice.The results of drug susceptibility test showed that the isolate was most sensitive to the quinolone drugs norfloxacin and ciprofloxacin,and had different degrees of resistance to other drugs.The anti-coagulation smears of sick racoon dog proved to be infected by Eperythrozoon,and the infection rate of red blood cells reached more than 98%.In summary,the pathogenic strain of the disease infection was E.coli and the E.coli was pathogenic,at the same time,the sick raccoon dog suffered from Eperythrozoon and led to its growth retardation.

Key words: racoon dog; Eperythrozoon; Escherichia coli; diagnosis and identification

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