猪圆环病毒2型靶细胞及其受体的研究进展

›› 2013, Vol. 40 ›› Issue (1): 25-28.

猪圆环病毒2型靶细胞及其受体的研究进展

李鹏^1,2, 王利平¹, 朱艳平¹, 王选年^1,2, 张改平²

1. 新乡学院生命科学与技术系, 生物技术研究中心, 河南新乡 453003;
2. 河南省农业科学院, 农业部动物免疫学重点实验室, 河南省动物免疫学重点实验室, 河南郑州 450002

收稿日期:2012-06-25 出版日期:2013-01-20 发布日期:2013-01-14
通讯作者: 王选年(1969-),男,河南人,教授,硕士生导师,主要从事动物免疫学研究。张改平(1960-),男,河南人,院士,博士生导师,主要从事动物免疫学和兽药残留研究。
作者简介:李鹏(1976-),男,河南人,博士,研究方向:动物病毒学与分子免疫学。
基金资助:
河南省科技攻关项目(122102110142);中国博士后科学基金资助项目(2011M501179)。

Recent Research Advances in Target Cell of Porcine Circovirus Type 2 and its Cell Receptor

LI Peng^1,2, WANG Li-ping¹, ZHU Yan-ping¹, WANG Xuan-nian^1,2, ZHANG Gai-ping²

1. Biotechnology Research Center, Department of Life Science and Technology, Xinxiang University, Xinxiang 453003, China;
2. Henan Provincial Key Laboratory of Animal Immunology, Key Laboratory of Animal Immunology, Ministry of Agriculture, Henan Academy of Agricultural Sciences, Zhengzhou 450002, China

Received:2012-06-25 Online:2013-01-20 Published:2013-01-14

摘要/Abstract

摘要： 猪圆环病毒2型(porcine circovirus type 2,PCV2)是统称为猪圆环病毒相关疾病(porcine circovirus-associated disease,PCVAD)的几个综合征的主要致病因子。目前,对PCV2配体表位和细胞受体在病毒入侵过程中的致病机理还不清楚,受体识别是病毒黏附到宿主细胞表面的第一步,在病毒入侵和致病机理方面起到重要的作用。作者对近几年来PCV2侵染的靶细胞及其靶细胞受体的研究进展作一综述。

关键词: 猪圆环病毒2型; 靶细胞; 细胞受体

Abstract: Porcine circovirus type 2 (PCV2) is the primary causative agent of several syndromes collectively known as porcine circovirus-associated disease (PCVAD). However, the role of ligand epitope and cell receptor in pathogenesis of PCV2 is still uncertain.Receptor recognition is the first step in viral entry, and plays an important role in viral infectivity and pathogenesis.This paper reviews the current knowledge relating to interactions between PCV2 and its target cell surface receptors.

Key words: porcine circovirus type 2; target cell; cellular receptor

中图分类号:

S852.65

李鹏, 王利平, 朱艳平, 王选年, 张改平. 猪圆环病毒2型靶细胞及其受体的研究进展[J]. , 2013, 40(1): 25-28.

LI Peng, WANG Li-ping, ZHU Yan-ping, WANG Xuan-nian, ZHANG Gai-ping. Recent Research Advances in Target Cell of Porcine Circovirus Type 2 and its Cell Receptor[J]. , 2013, 40(1): 25-28.

参考文献

1 Allan G M, McNeilly F, Ellis J, et al. PMWS: Experimental model and co-infections[J]. Vet Microbiol,2004,98(2): 165~168.
2 Cardin A D, Weintraub H J. Molecular modeling of protein-glycosaminoglycan interactions[J]. Arteriosclerosis,1989, 9(1):21~32.
3 Chakraborty S, Veettil M V, Chandran B. Kaposi’ arcoma associated herpesvirus entry into target cells[J]. Front Microbiol,2012,3:6.
4 Finsterbusch T, Mankertz A. Porcine circoviruses——small but powerful[J].Virus Res,2009a,143(2):177~183.
5 Finsterbusch T, Steinfeldt T, Doberstein K, et al.Interaction of the replication proteins and the capsid protein of porcine circovirus type 1 and 2 with host proteins[J]. Virology,2009b,386(1):122~131.
6 Grove J, Marsh M. The cell biology of receptor-mediated virus entry[J]. J Cell Biol,2011,195(7):1071~1082.
7 Inoue T, Moore P, Tsai B. How viruses and toxins disassemble to enter host cells[J]. Annu Rev Microbiol,2011,65:287~305.
8 Kekarainen T, Montoya M, Dominguez J, et al. Porcine circovirus type 2 (PCV2) viral components immunomodulate recall antigen responses[J]. Vet Immunol Immunopathol,2008,124(1~2):41~49.
9 Khayat R, Brunn N, Speir J A, et al. The 2.3-angstrom structure of porcine circovirus 2[J]. J Virol,2011,85(15):7856~7862.
10 Lefebvre D J, Meerts P, Costers S, et al. Increased porcine circovirus type 2 replication in porcine leukocytes in vitro and in vivo by concanavalin A stimulation[J]. Vet Microbiol,2008,132(1~2): 74~86.
11 Li J, Yu Q, Nie X, et al. Effects of porcine circovirus type 2 on expression of mRNA associated with endogenous antigen processing and presentation in pulmonary alveolar macrophages and circulating T lymphocytes in piglets[J]. Vet J,2012,193(1):199~205.
12 Marsh M, Helenius A. Virus entry: Open sesame[J]. Cell,2006,124(4):729~740.
13 Mateusen B, Maes D G, Van Soom A, et al. Effect of a porcine circovirus type 2 infection on embryos during early pregnancy[J].Theriogenology,2007,68(6):896~901.
14 Mateusen B, Sanchez R E, Van Soom A, et al. Susceptibility of pig embryos to porcine circovirus type 2 infection[J]. Theriogenology,2004,61(1):91~101.
15 Meerts P, Misinzo G, McNeilly F, et al. Replication kinetics of different porcine circovirus 2 strains in PK15 cells, fetal cardiomyocytes and macrophages[J]. Arch Virol,2005,150(3):427~441.
16 Misinzo G, Delputte P L, Lefebvre D J, et al. Porcine circovirus 2 infection of epithelial cells is clathrin-, caveolae- and dynamin-independent, actin and Rho-GTPase-mediated, and enhanced by cholesterol depletion[J]. Virus Res,2009,139(1):1~9.
17 Misinzo G, Delputte P L, Meerts P, et al. Porcine circovirus 2 uses heparan sulfate and chondroitin sulfate B glycosaminoglycans as receptors for its attachment to host cells[J]. J Virol,2006,80(7):3487~3494.
18 Misinzo G, Meerts P, Bublot M, et al. Binding and entry characteristics of porcine circovirus 2 in cells of the porcine monocytic line 3D4/31[J]. J Gen Virol,2005,86(7):2057~2068.
19 Nauwynck H J, Sanchez R, Meerts P, et al. Cell tropism and entry of porcine circovirus 2[J]. Virus Res,2012,164(1~2):43~45.
20 Rothenberg E, Sepúlveda L A, Skinner S O, et al. Single-virus tracking reveals a spatial receptor-dependent search mechanism[J]. Biophys J,2011,100(12):2875~2882.
21 Saha D, Lefebvre D J, Van Doorsselaere J, et al. Pathologic and virologic findings in mid-gestational porcine foetuses after experimental inoculation with PCV2a or PCV2b[J]. Vet Microbiol,2010,145(1~2):62~68.
22 Sanchez Jr R E, Meerts P, Nauwynck H J, et al. Change of porcine circovirus 2 target cells in pigs during development from fetal to early postnatal life[J]. Vet Microbiol,2003,95(1~2):15~25.
23 Steiner E, Balmelli C, Herrmann B, et al. Porcine circovirus type 2 displays pluripotency in cell targeting[J]. Virology,2008,378(2):311~322.
24 Timmusk S, Fossum C, Berg M. Porcine circovirus type 2 replicase binds the capsid protein and an intermediate filament-like protein[J]. J Gen Virol,2006,87(11):3215~3223.
25 Wu K, Peng G, Wilken M, et al. Mechanisms of host receptor adaptation by severe acute respiratory syndrome coronavirus[J]. J Biol Chem,2012,287(12):8904~8911.

[1]	刘新月, 栗利芳, 王振豹, 张文亮, 张欣, 赵洪涛, 李鹏宇, 郑昊天, 肖进, 齐鹏. 猪圆环病毒2型合成肽疫苗佐剂筛选与免疫保护性研究[J]. 中国畜牧兽医, 2023, 50(3): 1068-1080.
[2]	周文锋, 张英婕, 白一涵, 张露华, 麦金辉, 王乃东, 杨毅, 湛洋, 王东亮. 猪圆环病毒2型全基因组序列分析及Cap蛋白CTL表位预测[J]. 中国畜牧兽医, 2023, 50(2): 656-665.
[3]	周伟, 刘旭平, 张艳敏, 彭雯娟, 赵亮, 谭文松. 无血清悬浮培养PK-15细胞及其对猪圆环病毒2型增殖的影响[J]. 中国畜牧兽医, 2022, 49(6): 2397-2405.
[4]	羊露露, 李安琪, 袁生, 黄良宗, 黄淑坚, 温峰, 郭锦玥. 广东省4株PCV2型分离株全基因组进化分析[J]. 中国畜牧兽医, 2022, 49(3): 1015-1023.
[5]	张敬寒, 李枝兰, 韩佃刚, 何帅, 刘金华, 吕念词, 邹丰才, 柴俊, 张以芳. 稳定表达IL-10的PK-15细胞的构建及其在PCV2增殖中的应用[J]. 中国畜牧兽医, 2022, 49(3): 1085-1095.
[6]	孙庆帅, 陈燕虹, 姚伦, 孙琪, 于学祥, 吴昊, 阮胜男, 雷铭楷, 严伟东, 何启盖. 猪圆环病毒2型和3型混合感染引起的母猪繁殖障碍诊治[J]. 中国畜牧兽医, 2022, 49(10): 3982-3993.
[7]	曹迷霞, 关静渊, 杨剑, 王新蕊, 王玲力, 宾秀娟, 王可菲, 黄海资, 胡庭俊. 西番莲多糖提取物对猪圆环病毒2型感染RAW264.7细胞氧化应激的影响[J]. 中国畜牧兽医, 2021, 48(6): 2238-2246.
[8]	高映雪, 薛瑞, 林敏, 胡换仪, 刘昌锦, 万文忠, 罗锋, 邓舜洲. 表达猪圆环2型的多拷贝P28-Cap重组猪痘病毒的构建及表达量分析[J]. 中国畜牧兽医, 2021, 48(3): 818-828.
[9]	邓同炜, 薛亚南, 卢建洲, 夏艳勋, 陈露露, 郭懿文, 彭丽明, 蒋增海, 乔宏兴, 徐耀辉, 张晓战. 猪繁殖与呼吸综合征病毒、猪圆环病毒2型与副猪嗜血杆菌混合感染的诊断及病原分析[J]. 中国畜牧兽医, 2020, 47(10): 3401-3409.
[10]	王席, 李国攀, 陈清清, 徐保娟, 荣俊. 猪圆环病毒2型病毒样颗粒的纯化及鉴定[J]. 《中国畜牧兽医》, 2019, 46(6): 1792-1800.
[11]	蒋新华, 周荷田, 罗锋, 杨丹凤, 张志庆, 陈松昌, 邓舜洲. 2013~2017年江西省猪圆环病毒2型分子流行病学调查[J]. 《中国畜牧兽医》, 2019, 46(4): 1143-1150.
[12]	李基棕, 张冬霞, 李丽, 薛涛, 刘传敏. NO自由基对猪圆环病毒2型复制的体外抑制作用研究[J]. 《中国畜牧兽医》, 2019, 46(3): 924-930.
[13]	徐国, 梁海英, 曾智勇, 王彬, 黄涛, 汤德元, 代振江, 叶百川, 张爱琼, 何小莉, 咸文. V5标记的猪圆环病毒2型Cap蛋白重组杆状病毒表达载体的构建[J]. 《中国畜牧兽医》, 2018, 45(7): 1775-1782.
[14]	王利丽, 郑丽, 路超, 任卫科, 张莉, 李富强, 鄢明华. 猪圆环病毒2型天津及周边地区分离株的遗传演化分析[J]. 《中国畜牧兽医》, 2018, 45(5): 1331-1340.
[15]	王利丽, 路超, 任卫科, 韩伟, 李富强, 郑丽, 鄢明华. 天津及周边地区猪圆环病毒2型流行情况调查及分离鉴定[J]. 《中国畜牧兽医》, 2018, 45(4): 1027-1034.