耐氟喹诺酮类药物鸡毒支原体DNA回旋酶及拓扑异构酶Ⅳ的突变特征

›› 2010, Vol. 37 ›› Issue (9): 43-47.

耐氟喹诺酮类药物鸡毒支原体DNA回旋酶及拓扑异构酶Ⅳ的突变特征

魏飞龙，周云雷，沈祥广，蒋红霞

（华南农业大学兽医学院广东省兽药研制与安全评价重点实验室，广州 510642）

收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2010-09-20 发布日期:2010-09-20
通讯作者: 蒋红霞

Characterization of Mutations in DNA Gyrase and Topoisomerase Ⅳ Involved in Quinolone Resistance of Clinical Mycoplasma gallisepticum Isolates

WEI Fei-long, ZHOU Yun-lei,SHEN Xiang-guang,JIANG Hong-xia

(Guangdong Key Laboratory for Veterinary Drug Development and Safety Evaluation,College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China)

Received:1900-01-01 Revised:1900-01-01 Online:2010-09-20 Published:2010-09-20
Contact: JIANG Hong-xia

摘要/Abstract

摘要： 采用二倍稀释法测定临床分离的4株鸡毒支原体对常用抗菌药物的敏感性，PCR方法和基因测序法对鸡毒支原体DNA回旋酶编码基因gyrA、gyrB及拓扑异构酶Ⅳ编码基因gyrC和gyrE耐药决定区进行分析。敏感性测定结果表明，4株分离鸡毒支原体对泰乐菌素、泰妙林、沃尼妙林和替米考星有很高的敏感性，对四环素和红霉素中度敏感，对林可霉素、氟苯尼考和氟喹诺酮类药物呈现不同程度的耐药性。4株耐氟喹诺酮类药物鸡毒支原体均在GyrA和ParC的喹诺酮类耐药决定区（QRDR）发生氨基酸的改变，GyrA的氨基酸取代模式有两种，分别为Ser81→Gly和Ser83→Ile，ParC仅在80位发生氨基酸取代(Ser80→Leu)，GyrB和ParE均未发生氨基酸改变。

关键词: 鸡毒支原体; 最低抑菌浓度; DNA回旋酶; 拓扑异构酶Ⅳ

Abstract: Susceptibilities of 4 isolated M. gallisepticum to common used antimicrobials were determined by broth dilution method. PCR and sequencing were applied for analysis of quinolone resistance-determining regions of the four target genes encoding DNA gyrase and topoisomerase Ⅳ from 4 isolates. The results showed that 4 isolates presented higher susceptible to tylosin, tiamulin, valnemulin and tilmicosin,moderate susceptible to tetracycline and erythromycin, and resistant to lincomycin, florfenicol and 3 fluroquinolone drugs to a certain extent. Several mutations were found in the two fluoroquinolone targets GyrA and ParC. Double mutations within QRDR of GyrA were found among 4 isolated M. gallisepticum, with the same substitution patterns of Ser81→Gly and Ser83→Ile. There was only one alteration existed within QRDR of ParC (Ser80→Leu) among 4 isolates. No mutations were found within QRDR of GyrB and ParE in 4 isolates.

Key words: Mycoplasma gallisepticum; minimum inhibitory concentration; DNA gyrase; topoisomerase Ⅳ

中图分类号:

S859.7

魏飞龙;周云雷;沈祥广;蒋红霞. 耐氟喹诺酮类药物鸡毒支原体DNA回旋酶及拓扑异构酶Ⅳ的突变特征[J]. , 2010, 37(9): 43-47.

WEI Fei-long;ZHOU Yun-lei;SHEN Xiang-guang;JIANG Hong-xia. Characterization of Mutations in DNA Gyrase and Topoisomerase Ⅳ Involved in Quinolone Resistance of Clinical Mycoplasma gallisepticum Isolates[J]. China Animal Husbandry & Veterinary Medicine, 2010, 37(9): 43-47.

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