›› 2010, Vol. 37 ›› Issue (7): 128-131.

• 遗传繁育 • 上一篇    下一篇

5个猪种DECR1基因外显子2单链构象多态性的群体遗传学分析

李根银1,王效京1,曹宁贤1,刘宏2,张维军3,曹果清4,李步高4   

  1. (1.山西省畜禽繁育工作站,太原 030000;2.大同市种猪场,大同 037000;3.山西省长治市畜牧局,长治 046000;4.山西农业大学动物科技学院,太谷 030801)
  • 收稿日期:1900-01-01 修回日期:2010-06-18 出版日期:2010-07-20 发布日期:2010-07-20
  • 通讯作者: 李步高

Population Genetic Analysis of Polymorphisms on Exon 2 of DECR1 Gene in 5 Pig Breeds

LI Gen-yin1,WANG Xiao-jing1,CAO Ning-xian1,LIU Hong2,ZHANG Wei-jun3,CAO Guo-qing4,LI Bu-gao4   

  1. (1.Shanxi Livestock and Poultry Breeding Station,Taiyuan 030000,China;2. Datong Pig Breeding Farm in Shanxi Province,Datong 037000,China;3. Bureau of Livestock of Changzhi City in Shanxi Province,Changzhi 046000,China;4. College of Animal Science and Veterinary Medicine,Shanxi Agricultural University,Taigu 030801,China)
  • Received:1900-01-01 Revised:2010-06-18 Online:2010-07-20 Published:2010-07-20
  • Contact: LI Bu-gao

摘要: 试验利用PCR-SSCP技术检测了山西马身猪、山西白猪、长白猪、大白猪和杜洛克猪5个品种共490头猪的DECR1基因外显子2的多态性。结果显示,DECR1基因外显子2位点存在多态性,表现出AA、BB和AB 3种基因型。群体遗传学分析结果表明,5个猪种都是等位基因B为优势基因;Hardy-Weinberg平衡检验结果显示,山西白猪和杜洛克猪处于平衡状态,山西马身猪、长白猪和大白猪处于非平衡状态;各品种猪的多态信息含量均为中度多态。

关键词: DECR1基因; PCR-SSCP; 多态性;

Abstract: The polymorphisms of the 2,4-dienoyl CoA redutase 1 (DECR1) gene in exon 2 site was detected using PCR-SSCP in 490 pigs of 5 pig breeds including Shanxi Mashen, Shanxi White, Landrace, Yorkshire and Duroc. The results showed that there was a polymorphism in the exon 2 site and three genotypes (AA, BB and AB) were found. Analysis of population genetics suggested that the frequency of B allele was dominant among the studied breeds. The results of Hardy-Weinberg equilibrium test indicated that Shanxi White and Duroc reached the equilibrium. All of the breeds had intermediate polymorphism information contents.

Key words: DECR1 gene; PCR-SSCP; polymorphism; pig

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