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Table of Content
20 May 2007, Volume 34 Issue 5
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动物营养与饲料科学
Molecular Properties and Gene Expression Mechanism of Lactoferrin
CHENG Jinbo;WANG Jiaqi;BU Dengpan
2007, 34(5): 5-9.
Abstract
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1282
)
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The lactoferrin， an iron-binding glycoprotein， exits widely in secretions of mammals.It reported that lactoferrin has many physiological functions. Some research of lactoferrin on lolecular properties, physiological functions, action and gene expression regulating mechanism were reviewed in this paper.
Progress of Research on Effects of Polysaccharides on Macrophage
RONG Yueguang;TONG Jianming
2007, 34(5): 9-13.
Abstract
(
1452
)
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Macrophages play an important role in host defense in phagocytosis, cytotoxicity, antipresenting, cytokines secretion, regulating internal environment and antitumor effects. Previous studies showed that polysaccharides had the effects in antitumor and modulating macrophage activities. More and more polysaccharides were developed for its immune modulating activities in macrophage and the recent researches mainly focused on investigating the receptors and signal transduction mechanism responsible for the immune modulation by polysaccharides.
Advances in Regulating Mechanism of AMPK on Nutrient
BI Jinming;ZHANG Minhong;WANG Yongjun
2007, 34(5): 14-17.
Abstract
(
1336
)
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It has been confirmed that AMPK can perceive the change of metabolism state of cell and maintain the cell energy equilibrium through some ways. At the same time, many researches have indicated the existence of the relevance between the activation of AMPK by stress factors and the nutritional metabolic disease. Thus, this review provides the solid theory foundation for research of nutrient regulation and stress regulation through narrating its nutrient regulating functions and other physiological regulating characters.
Effect of Cysteamine on Immune Function in Piglets
LIU Zhi;XU Xuesong;LIU Li;LI Jian;WANG Xichun;WU Jinjie
2007, 34(5): 18-21.
Abstract
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1320
)
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Different dosage of cysteamine(CS) were added to the basal diets to study the effect of CS on immunity in piglets. Ninety newborn “Duroc× Landrace×Yorkshire” crossbred piglets were divided into 3 groups with 30 piglets each，and fed with basal diet, basal diet plus 120 mg/kg CS and basal diet plus 200 mg/kg CS， respectively. The trial was conducted for 45 d. Blood samples in 20 piglets each group were taken on 14,24,32,42 day for measuring some immune indices. The results indicated that addition of cysteamine to the basal diet increased significantly the weight and average daily gain, decreased the ratio of feed to gain in piglets, increased significantly the levels of serum IgA,IgG，phagocytosis rates of neutral granular cells and conversion rates of T- lymphocytes. Basal diet with 120 mg/kg CS could improve growth performance more preferably, and with 200 mg/kg CS could enhance the immunity of piglets more significantly. The trial implied that appropriate dosage of CS could effectively inhibit weanling stress and enhance immunity of piglets.
Recent Research Advances of Cloning Transgenic Pigs Rich in Omega-3 Fatty Acids
PAN Dengke;CHENG Hongxing;FENG Shutang;LI Kui;DENG Jixian
2007, 34(5): 22-25.
Abstract
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1892
)
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In this paper, the significance and rencent progress of transgenic pigs rich in omega-3 fatty acids are presented. In our study，the
sFat
-1 gene was chemically synthesized according to the sequence from
Caenorhabditis briggssae
，and constructed into a mammal expression vector pcDNA3.1
sFat
-lEGFP. Transgenic
sFat
-1 gene mice were generated by microinjection of vector into pronuclear zygotes. A modified n-3 fatty acid desaturase gene from
C. Briggsae
produced high proportion of DHA and DPA in transgenic mice. Based on Function Analysis of n-3 Fatty Acid Desaturase Gene
sFat
-1, porcine fetal fibroblasts were transfected with vector pcDNA3.1
sFat
-lEGFP. Positive colony was chosed as donors for nuclear transfer to enucleated MⅡoocytes, cloning transgenic pigs rich in omega-3 fatty acids.
The Influence of the Different Drying Methods on Nutritive Value of Alfalfa in High Plateau Area
HAI Cunxiu
2007, 34(5): 26-28.
Abstract
(
1262
)
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In this paper,the influence of different drying methods on the nutritive value of alfalfa were researched.The results demonstrated that steam-pressing combined with sun-curing could make shorten the drying time and reduce the loss of CP,Ca and P of alfalfa hay,even could keep nutritive value and improve the quality of alfalfa hay.
Study in Effect of Cysteamine of Different Dosages on the Productivity of the FuzzWooled Sheep in the Northeast China
WANG Hongcai
2007, 34(5): 29-30.
Abstract
(
1908
)
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In this test ,32 female Kids of the Fuzz-Wooled Sheep in the Northeast China are divided into four groups at random one of which was used as the contrast group and fed with the basic daily food supplies and the remaining 3 groups were fed with 75,125,175 mg/kg Cysteamine in the daily food supplies respectively . The findings indicate that on average, the test group with 125 mg/kg Cysteamine produced the best result of enabling the kids to increase their weight by 20.96%（P≤0.01）every day and the wool length by 31.51%（P≤0.01）, decrease the ratio of the feed to the pork by 15.79%（P≤0.01） and increased the daily income by 66.07%（P≤0.01）.
Determinatiaon of Sulfonamides Residues in Chicken by TCL
LIU Xuehong;LI Juan;DONG Ziling;SONG Ping;HAN Fengli;MA Ping
2007, 34(5): 31-33.
Abstract
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763
)
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A method of TCL was developed to determine sulfonamides residues in chicken,Sulfonamides were extracted from tissues by dichloromethane and distilled to dry. A silicon G board was used as stationary phase,the mixture of (chloro- form-Trimethyl carbinol) (85∶15)as developping solution,and 0.01% Fluram as revealing agent.The bands were clear without interference.The average recovery was 60%，The detection limit was 0.05 μg/ml.
奶业专栏
Application of Biological Agents for Preventing and Curing Dairy Cow Mastitis
YI Mingmei;FENG Hua;ZHU Jianguo;MENG He;HUA Xiuguo;SUN Huaichang;YUAN Yaoming
2007, 34(5): 37-39.
Abstract
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1482
)
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Dairy cow mastitis has been considered as a major and world-wide problem in the cow industry production. This review discusses the currently available information on the function of cytokine, human lysozyme and vaccine on preventing and curing dairy cow mastitis one by one, which may provide the theory basis for preventing and curing this disease.
生理生化
The Effect of Cholinesterase on Nitrobenzene Induced Neurotoxicity in Mouse
XING Houjuan;WANG Min;XU Shiwen
2007, 34(5): 46-48.
Abstract
(
1339
)
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In order to explore the changing rule of the cholinesterase caused by nitrobenzene poisoning. Once a day, we fed mice with different doses of cholinesterase 30 days in all. The activity of cholinesterase in brain and the quantity of cholinesterase in liver were detected by alky hydroxylamine chromometry. The clinical manifestation of mice were observed during the test.The results indicated that mice show various symptomes such as slow action, obtuse reaction, hyperspasmia and abnormal emotion for example excition, saitivity and continual jump. These symptomes are increasingly obvious with the progressive degree of intoxation and time passing. The activity of cholinesterase in brain was depressed while the quantity of cholinesterase in liver increasing. We concluded that nitrobenzol is neurovirulent and it is able to inhibit the activity of cholinesterase in both liver and brain which displays evident dosage effect.
Progress on GonadotropinReleasing Hormone
CAO Jing;CHEN Yaoxing;WANG Zixu;LI Junying
2007, 34(5): 48-51.
Abstract
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1246
)
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The structure and biological function of gonadotropin releasing hormone have been one of the research focuses on neuroendocrine and reproductive biology. Our paper was briefly reviewed the structure, category, distribution, signal conduction and function of gonadotropin releasing hormone.
Antiinflammatory Effects of Berberine
YE Baona;HAO Manliang;LIU Ping;XIE Fei
2007, 34(5): 52-53.
Abstract
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1366
)
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Berberine has various pharmacological effects. Recent years, more and more studies showed that berberine has a antiinflammatory effects. The paper reviews anti-inflammatory effects and mechanism of berberine.
遗传繁育
Study on Four Microsatellite DNA Polymorphism in the Region of
GDF
8 on Chromosome 2 in Meat Sheep Populations
SONG Yanhong;SHI Guoqing;LIU Nan;DAI Rong;REN Hangxing;LIU Guoqing
2007, 34(5): 54-56.
Abstract
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1684
)
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According to report, four highly polymorphic microsatellite loci possibly linked to production traits and mapped to the region of
GDF
8
on sheep chromosome 2 were selected. they were
BM8
1124，
TEXAN
2，
FCB
128，
BMS
1591. They were analyzed for polymorphism in Dorset,Suffolk and Texel meat sheep populations. Results showed that the number of alleles were 10,12,7,11 and polymorphic information content / Heterozygosity were 0.7778/0.8045,0.712/0.7520,0.7551/0.7852,0.8057/0.8289.We can see these microsatellite loci have rich polymorphism in three groups.So they can be used as the linkage analyse between the production traits and makers,and used for further location of QTL.
Effects of EGF and IGF-1 on Maturation of Buffalo Oocytes in Vitro
WANG Xiaoli;NONG Yaofeng;FENG Guixue;SHI Deshun;WEI Jingwei;ZHOU Hongying
2007, 34(5): 57-59.
Abstract
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1284
)
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Three experiments were conducted to determine the effect of EGF and IGF1 on maturation of buffalo oocytes in vitro. Oocytes were cultured in oocytes maturation medium with EGF and IGF-1 alone or combinedly at different concentration. The results in experimentⅠshowed that there were significant different between control group and the group with EGF(10，20，30，50 ng/ml) in maturation rate, the group with 50 ng/ml EGF was significantly higher than others(P＜0.05). In experiment II the maturation rate of buffalo oocytes was improved significantly in the groups with IGF(30 ng/ml)(P＜0.05), but not in the group with 10 ng/ml IGF-1. In experiment III the maturation rate in the group containing 20 ng/ml EGF and 30 ng/ml IGF-1 was significantly higher than 30 ng/ml IGF-1 or 20 ng/ml EGF groups(P＜0.05). The results obtained in this study indicate that EGF and IGF-1 have coeffects on maturation of buffalo oocytes in vitro.
Molecular Cloning， Sequencing and Constructing Eukaryotic Expression Vector of Neijiang Pig IFN-γ
HUANG Yaping;GUO Wanzhu;LI Xiaoqi
2007, 34(5): 60-63.
Abstract
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1433
)
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Total RNA was isolated from neijiang pig peripheral blood lymphocytes which were stimulated with ConA. Then the pIFN-γ cDNA was amplified by reverse transcription polymerase chain reaction. The amplified fragment was cloned into vector pMD18-T named pMD-N-IFN-γ and then sequenced. It was found that the amplified fragment was consisted of 603 nucleotides, the ORF consisted of 501 nucleotides, encoding 166 amino acids, Neijang pig IFN-γ gene has more than 99.4% homology with other pigs on Genbank. Subcloning the ORF of pig IFN-γ gene， then the sub-cloned gene was successfully inserted between EcoRI and XhoI site of the eukaryotic expression vector pcDNA3.1(+)， named pcDNA3.1(+)-N-IFN-γs.
Isolation, Purification and Identification of Testis Sertoli Cells in Adult Rabit
ZHANG Jing;ZHOU Zhihui;ZOU Haijun;WANG Xinglong
2007, 34(5): 63-66.
Abstract
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1351
)
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To found a method of getting much more high purity and high viability sertoli cells from adult rabbits’ testis，we used three enzymes which were collagenase,trypsin and hyaluronidase, digested with two steps, at last, isolated sertoli cells from adult rabbits. Then，we treated those cells with hypotonic shock in Tris-HCl, and through selected adhibition cultured, the sertoli cells were sublimated. At the same time,we used morphologic and HE dye to identify them. The result: we got plenty of sertoli cells which were high purity(78.3%)and high viability(90.7%).
Polymorphism Analysis of the Exon 2 of SLA-DRB Gene ofTibet Pig by PCR-RFLP
JU Huiping;WU Shenglong;SUN Pengxiang;BAO Wenbin;XU Shenghai;LI Bichun;CHEN Guohong
2007, 34(5): 67-69.
Abstract
(
1927
)
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The exon 2 of SLA-DRB gene in Tibet pigs was analyzed by PCR-RFLP method. The results showed that the exon 2 digested by restriction endonuclease RsaⅠcould be divided into four kinds of alleles and seven kinds of genotypes in Tibet pigs respectively, and the frequency of BC was the highest(0.4419). The results of Chi Square test indicated that the frequencies of patterns digested by RsaⅠ were not fit in Hardy-Weinberg equilibrium in Tibet population(P<0.05).
Studies on Carcass Characteristics and Meat Quality of American Strain Rex Rabbit
XIONG Guoyuan;ZHU Xiubai
2007, 34(5): 69-74.
Abstract
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1480
)
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20 American strain Rex rabbits were slaughtered to study the characteristics and correlations of carcass and meat quality. The results showed: the average weight, commercial skin weight, dressing percentage, right hind leg weight, length and circumference length were 2.533 kg, 0.410 kg, 60.70%, 0.182 kg, 30.69 cm and 18.04 cm respectively about 150 days. The Rex rabbit longissimus lumborum and biceps femoris muscles pH of 1 h postmortem (pH1) were 6.42 and 6.43 separately, and pH of 24 h postmortem (pH24) were 6.22 and 6.35 separately. The L*values, a*values and b*values of longissimus lumborum muscle were 54.66, 12.37 and 9.06 respectively, and those of biceps femoris were 52.67, 9.89 and 4.08 respectively. The drip loss, ripe meat ration, cooking loss, Warner-Bratzler Shearing value, water content and intramuscular fat were 2.06%，62.12%，30.44%，2.453 kg.f，73.60% and 1.67% respectively. The positive correlations of commercial skin weight to hot carcass weight, length were high significant (P<0.01), and the positive correlation of commercial skin weight to commercial carcass weight was significant (P<0.05). Weight（hot carcass weight, commercial carcass weight and right hind leg weight）, body figure (length and circumference) to drip loss, cooking loss were negative correlations, and to ripe meat ration, WarnerBratzler Shearing value were positive correlations, but there were not significant (P>0.05).
The Effect of Bovine Oviductal Epithelial Cells on Mouse Embryonic Development
FANG Changge;MIAO Xiangyang;YANG Wensheng
2007, 34(5): 75-77.
Abstract
(
1595
)
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17β-estradiol and progesterone were added into TCM-199 medium to inv
estigate the effects of steroids on the secretion of bovine oviductal epithelial
cells (BOECs) in vitro.The results of 5% SDS-PAGE showed that the steroids could induce the BOEC secretion.When the proteins secreted by BOECs (BOEPs) were added into the cultural medium CZB for mouse embryos, we observed that BOEPs could significantly promote the mouse embryos to develop beyond the 2-cell inhibition and to develop more blastula than the proteins from the adult bovine oviduct (BOP) and the proteins from the calf oviduct (COP) (Ｐ<0.001).But the fact that the percentage of mouse blastula in BOEP group was lower than the control suggests the BOEPs be short of some proteins, which could promote the cooperation of many proteins better.In addition, the proteins of 30 kD and 56kD in BOEPs may inhibit the embryonic development.
The Characteristic,Identify,Mechanism and Function of MiRNAs
YANG Rongsheng;ZHANG Yamei
2007, 34(5): 77-80.
Abstract
(
1256
)
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Recent work has demonstrated that miRNAs are involved in critical biological processes by suppressing the translation of coding genes.This regulation
occurs at a posttranscriptional,presumably translational level and is distinct from RNA interference.Researches suggested that they are conserved in size,structure and function.The characteristic,identify,mechanism and function of miRNA are mainly reviewed.
疾病防治
On Rapid Diagnosis and Vaccine Research of Peste Des Petits Ruminants
LI Gang;JIANG Yanzeng;CAO Shengyu;ZHANG Jinxiu;ZHU Hongfei;SHI Jianzhong
2007, 34(5): 81-84.
Abstract
(
1322
)
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Peste des petits ruminants (PPR) is a highly contagious and infectious viral disease of domestic and wild small ruminants included in the CLASS A list of OIE. It is caused by peste des petits ruminants virus (PPRV), a Morbillivirus in family Paramyxoviridae, closely related to renderpest virus (RPV). As there is no cure for this disease, diagnostic technology plays a very important role in disease control. But conventional ways like the agar gel immunodiffusion (AGID), virus neutralization test (VNT), et al. could work well in the surveillance of PPR. Now new assays based on molecular biology techniques , like the RT-PCR. PCR- ELISA is the main trend. They were sensitive, high throughput, time-saving, and could operation in the field. Recombinant vaccines are researched and used at present.
Therapeutic for Prion Diseases with Immunology
ZHAGN Taixiang;ZHAO Deming
2007, 34(5): 86-87.
Abstract
(
1285
)
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Neuronal cell death is considered to be a hallmark in prion diseases. These disorders are believed to result from the post-translational conversionof a normal cell membrane sialoglycoprotein, composed primarily of helical structure, into a disease specific isoform, that is rich in sheet and partially proteinase-resistant.Recent in vivo studies indicate that prion replication can be inhibited by anti-PrP monoclonal antibodies that led to the indefinite delay in the development of prion disease.Furthermore, these reports increased momentum about the use of antibody-based therapy in prion diseases.
Preparation of Engineering
E Coli
DH
5a
Sensitive Cell and Study on the Plasmid pGEM Transformation
DU Zhen;MA Haili;CHEN Ruifang
2007, 34(5): 88-90.
Abstract
(
1287
)
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GFP to the optical bleaching, the oxidant, the reducing agent, the acid, the alkali and all that many chemical reagent has the greatly strengthened stability. First, from includes GFP in the backwoods coli to withdraw the material particle, and causes its purification using the rubber recycling reagent box. At the same time, makes using the calcium chloride law project fungus DH
5a
the feeling condition cell. Then will purify after the material particle to change over to already in the feeling condition cell which the system will arrange, will cause project fungus DH
5a
to transform has the anti-ammonia animal pen penicillin the backwoods coli, and the vaccination in on the ammonification animal pen penicillin culture medium, willcause its growth.
Doagnosis and Prevention of Infections Serositis in Ducks
ZHOU Zhenhui;LI Yubin;CAO Jinyuan;XIANG Shuangyun;NIE Changkun
2007, 34(5): 91-93.
Abstract
(
1418
)
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Infections serositis in ducks was diagnosesed accoding to the observetions of clinic symptom,pathological changes,experimental diagnosis in Beijing.PA(pasteurella anapestifer) was isolated from heads,hearts and livers of
diseased ducks.PA were cultivated and inactivated by HCHO and mixed with Al(OH)3. The 1-day-old ducks were immunized with 0.5 ml the inactivated vaccines,after 14 days, the ducks were immunized with 1 ml the inactivated vaccines again.The results showed that the vaccine were safe to the ducks,the caccined ducks obtained 95% protective efficacy.In drug sensitivity test, pasteurella anatipestifer was highly susceptible to neomycin and kanamycin.
Application of Monoclonal Antibody in Diagnosis of Animal Disease
ZHANG Chang;REN Xiangyang;LIU Qiuyan;WU Qing;CHENG Lu;WANG Chuanqing
2007, 34(5): 93-95.
Abstract
(
1164
)
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Monoclonal antibody techniques are very meaningful in modern life science research. This review mainly introduces the recent status of monoclonal anti
body and the application in diagnosis of animal diseases，and gives a discussion of the problems in monoclonal antibody researches.
Advance of Research and Clinical Application of the Traditional Chinese Medicine Immune Depressant
SHI Wanhua;WANG Jie;DIAO Yongqiang
2007, 34(5): 98-101.
Abstract
(
1447
)
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At present it is much shortcomings to cure autoimmune disease anaphylactic disease and so on by hormone clinically.So that the method above cant prevent the diseases from coming and development.Fortunatelly, because of little of toxic side effect and good curative effect, the traditional Chinese medicine immune depressant depending on mechanism of immune tolerance have been widely appled to clinic. Introducing foundation and affect of clinic on the traditional Chinese medicine immune depressant in latest years and raiseing opinion of shortage during the research and tendency of deveploment, it is necessary to exploit the traditional Chinese medicine immune depressant.
Comparative Characterstic of Infectious Bronchitis Virus M41 and Ma5 Cultured in Tracheal Organ
LIU Wei;HE Ying;CHEN Zhongwei
2007, 34(5): 103-104.
Abstract
(
1720
)
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The characteristic of IBV-M41 and IBV-Ma5 strains cultured in tracheal organ was assessed by the ciliary activity of chicken embryo tracheal organ. After the virus (containing 100TOCID50) was inoculated into trachealring organ cultures, the survival rates of cilia were assessed . The results showed that the survival rates of epithelial cells inoculated with IBV-Ma5 was high than with IBV-M41, which means their culture characteristics accord with their toxicity.
流行病防治
The Viral Diseases to Cause Porcine Immunosuppression and Control Measure
DENG Bowen
2007, 34(5): 117-119.
Abstract
(
1170
)
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It is one of the dangerousest and concealabest factors about threatenning porcine health now and future that immunosuppression causes immunity-fail and sensitiver disease. This article analysises to conception 、mechanism and control measures about the two porcine immunosuppressive viral diseases at present.
动物生产
Environmental Pollution, Integrated Prevention and Cure Countermeasure of the Scale Livestock or Poultry Farming
CHEN Xiaoou;JIA Yongquan;WANG Xiwen
2007, 34(5): 137-138.
Abstract
(
1268
)
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Environmental pollution of Scale livestock or poultry farming in our country is worse, it has intimidated living of livestock,poultry and people. The article analyzes the reason of contamination comprehensively, and gives prevention and cure countermeasure.
经验交流
The Establishment of Growth Model on Yunnan Simmental Cattle
DENG Weidong;LIU Wanghong;WANG Yanfeng;SU Huawei;MAO Huaming
2007, 34(5): 143-145.
Abstract
(
1855
)
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Various data of growth about Simmentals in Yunnan province shown that the difference of the natural weight between the original Simmentals and the Simmentals which were born in Yunnan later on three years was not significant (P>0.05), but were remarkable higher than the Simmentals which were born from 1999 to 2000; and at subsequent each age stage(0.5-9 years), the weight of the original Simmentals were remarkable higher than local pureblood Simmental (P<0.05),but along with the exaltation of feed management level. The weight and growth speed of the simmental which were born in 2004 get an improvement.
奥特奇专栏
Review on Glycomics Research-Research Progress on Structure and Function of Β-Glucans
Li Wankun;YAN Hongbin;CAI Xuepeng;GUO Fucun;
2007, 34(5): 152-155.
Abstract
(
1803
)
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Glycomics, a new subject, is specialized on research of structure and functional features of carbohydrates, especially the biological mode of actions by carbohydrates; therefore glycomics comes to be one of the hot topics of life science research. Polysaccharides and their derivers play a role of reserving various biological information during the whole life time and activities, and transferring substances intra and extra-cellular as well as between cells. At present, β-glucan is one of the most popular research subjects (i.e. carbohydrate research) in the area, among which the earliest sugar studied is Lentinan because of its immunoactivities and anticancer activities The article is a review on biological status of posaccharides, structure of β-glucans, and the relationship between the structure of βglucans and their biological functions. It is described in the article that biological activities of a glucan relate to the molar weight, solubility, branch chain, structure and crystal of the sugar. It was found that β-glucan has many different structures but one- and three-helixes are main forms of senior structures of β-glucan.
