IBV HN99strain was multiplied and purified by chick embryo kidney cell (CEKC), and the SPAFAS Jinan laboratory certified it was pure. Virus neutralization test (VN) revealed that the serotype of HN99strain was similar to IBV T strain. The inactivated vaccine of HN99 strain was prepared and it showed absolutely safety, moreover, chickens were immunized with the vaccine at different dose by muscle injection, three weeks later, 100 %protection were induced after challenging with HN99 strain, the results indicated that the IBV HN99 strain had fine Immunological Properties.