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Table of Content

20 April 2008, Volume 35 Issue 4
动物营养与饲料科学
Effects of Different Four Diets on Blood Biochemical Indexs for Crossbred Boer Goats
YANG Bingzhuang;LIANG Xianwei;BAO Fuyin;ZHENG Wei;LIANG Kun;ZOU Caixia;ZHENG Hongying;LI Lili;ZOU Longshu
2008, 1(4):  5-8. 
Abstract ( 1626 )  
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In order to give full play to the Boer goat heterosis,fattening period through research crossbred Boer goats of the wave energy and protein nutritional requirements parameters, screening of a better economic efficiency and the energy mix of protein feed supplements, with a view to keeping the scientific theoretical basis and provide production guidance. Sixteen fattening crossbred male Boer goats F1weaned at the age of 3 month were used to Study. A 4×4 Latin square experiment design was conducted to study the energy and protein requirements of stallfeeding crossbred Boer goats fed the four different diets based on recommendation of nutrients requirements of NRC(1981). The results show that: The concentration of BUN in plazma was significant difference (P<0.05) between diet 3 and diet 2,4; ALB concentrations in serum was significant difference (P<0.05) between diet 1 and diet 2,4; TG concentrations in serum was significant difference (P<0.05) between diet 1 and diet 4; The concentration of InS in plazma was significant difference(P<0.05) between diet 1 and diet 2,4,and there was significant difference(P<0.05) between diet 2 and diet 3; TCho concentrations in serum was significant difference(P<0.05) between diet 1 and diet 3,4;there was no significant difference (P<0.05) in other blood indexs. 
Selenized Yeast Supplementation of Lactation Dairy Cow: Effecting Slenium Concentration in Serum and Milk
WAN Lian-qun;;WANG Jia-qi;LUO Qiu-jiang;BU Deng-pan;ZANG Chang-jiang;ZHOU Zheng-fen;WANG Lei;XIA Nan; 
2008, 1(4):  13-17. 
Abstract ( 1921 )  
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The objective were to determine effects of selenized yeast on lactating Holstein cows performance and in selenium concentrations in the serum and milk. Twenty four lactating Holstein cows with similar parit days in milk, milk yield and body weight were blocked randomized in two groups. All cows were fed a control diet, contained 0.33 mg of selenium/kg of dry matter. Cows in control did not received selenized yeast, and cows in treatment received selenium 0.77 mg/kg of dry matter from yeast. The experimental period was durational for 56 d. There were no significant for animal performance between treatment and control. Selenium concentration of the serum and milk in treatment were 123.1 μg/L and 97.7 μg/L, respectively. In meantime, compared with control, the selenium concentration of serum and milk in treatment was increased by 60.5% and 194.3%, separately. In whole experimental period, the concentration seleium in serum was increase, whereas the concentration seleium in milk was not change after seleium reached a plateau.
The Nutrition Value of Forage Rice and Its Utilization on Livestock and Poultry
ZHANG Peihua;;HE Jianhua;WANG Jiaqi;LIU Chaogan
2008, 1(4):  17-21. 
Abstract ( 1809 )  
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The article reviewed the nutrients and digestibility of different fractions including paddy rice,brown rice and the straw and reviewed the studies of forage rice on the swine,poultry and the cattle in order to afford reference for use it on the dairy cows.
Effects of Different Supplementation Level and Different Adminstration time Length of Magnesium Asparatate on Meat Quality of Broiler Chickens
LIU Yong-xiang;;LIU Yan-li;LIU Tai-yu;GUO Yu-ming
2008, 1(4):  22-25. 
Abstract ( 1917 )  
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The experiment was conducted to investigate the effects of dietary magnesium asparatate(MgAsp) supplementation level and different administration time length on brisket quality of broiler chickens. In Experiment 1, two hundred and fifty six male AA broiler chicks were allocated to four treatments, each treatment with eight replicates of 8 chickens. The birds were fed with the basal diet alone or supplemented with 0.3%,0.6%,0.9% MgAsp. In experiment 2, the birds were fed with the basal diet supplemented with 0.9% MgAsp from the sixth week, fifth week and fourth week,respectively, and the basal diet without Mg supplementation served as the control. In Experiment 1, pH value of brisket increased quadratically as dietary supplemental MgAsp increased (P<0.01). TBARS value of brisket decreased linearly as MgAsp supplementation increased (P<0.01). Dietary MgAsp supplementation significantly increased LDH activity of (P<0.05). L value of brisket increased (P<0.05)and b value of brisket decreased as dietary MgAsp supplementation increased; In experiment 2, with the longer administration of MgAsp,except of TBARS of brisket,the quality of meat did not improve further. Dietary MgAsp supplementation can improve quality of meat, and the short time of MgAsp administration(1 week) is effective. 
生物技术
Cloning and Prokaryotic Expression of Growth Hormone cDNA from Vulpes Vulpes
LIU Yu-tang;HU Xiao-hang;;YAN Li-hui;CAO Dian-jun;LIU Pei-xin;YANG Chuan-ping
2008, 1(4):  26-30. 
Abstract ( 2144 )  
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To obtain the recombinant vulpes vulpes growth hormone. The mature vulpes vulpes growth hormone mRNA obtained from pituitary was amplified by RTPCR , cloned into vector pMD18-T and subcloned into prokaryotic expression vector pPROEXTMHta at restriction enzyme sites BamHⅠ and HindⅢ. The recombinant plasmids pMD18-TfGH cDNA and pPROEXTMHta-fGH cDNA were identified by restriction enzyme digestion, PCR and sequencing. Then we transfer positive recombinant prokaryotic plasmid into E.Coil, and the protein was expressed under the induction of IPTG at the final concentration of 1 mmol/L. The protein expression was measured by SDS-PAGE, and a high level of fGH protein was obvserved at 26 kD which occupied about 35% of total E.coil protein. The recombined fGH protein existed in the for
m of inclusion bodies.
The Advantage of Single Nucleotide Polymorphisms as a New Molecular Markers
WANG Shu-xin;LIAN Lin-sheng
2008, 1(4):  31-33. 
Abstract ( 1248 )  
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Single nucleotide polymorphism (SNP) that make up 90% of all genetic variations, inherited and do not change much from generation to generation, is now serving as a new molecular markers, can be of great value to genetics research and the studies is straightforward very much. So,this paper elaborated the advantage of SNP to gave a hand for its beening wellknown and further study.
遗传繁育
Clone and Sequence Analyse of the Gaoyuan Yak’s Lfcin Gene
PEI Jie;YAN Ping;JI Guo-hong;FENG Rui-lin;LIANG Chun-nian;GUO Xian;ZENG Yu-feng;BAO Peng-jia
2008, 1(4):  37-41. 
Abstract ( 1363 )  
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Using PCR technology, the lactoferricin gene was obtained from genome of Gaoyuan yak; The Lfcin gene was cloned to pGEM-T easy vector, and the product was sequenced by biocompany; Gaoyuan yak and cow’s Lfcin sequences were aligned; Yak, cow, human, mouse’s Lfcin protein sequences were analysed. As a result, the DNA sequence that contains the LF gene second exon of Gaoyuan yak was cloned, constituted by 778 bp, Lfcin coding region that code 25 amino acids being 75 bp length; According sequence analyse, there are 9 different bases between cow and yak’s sequence; Yak and cow’s Lfcin gene code same protein sequence and there are high homology in different species.
Uncoupling Protein and Polymorphism Regulation the Energy Metabolism
WEN Sheng-ping;ZHANG Li-chun;CAO Zhen-hui;LI Qi-hua;TIAN Yun-bo;CHAI Yan;CHEN Bao-ding;GE Chang-rong;JIA Jun-jing
2008, 1(4):  42-45. 
Abstract ( 1253 )  
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The uncoupling proteins are now known to be mitochondrial inner membrane proteins. Recently, more than five family members have been identified including UCP1,UCP2,UCP3,UCP4 and UCP5 in the mitochondrial inner membrane of different tissues and organs of species, such as in human, mammals, poultry, fish and even in plants. UCPs regulate the energy metabolism, increase the fatty acids oxidation and the UCPs polymorphism are related to fat metabolism. 

Comparison of Three Methods for Genomic DNA Extraction
LIU Yan;LIU Meng-zhou;XU Yan-xiang
2008, 1(4):  45-47. 
Abstract ( 1292 )  
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We separately used several different method to extracted genomic DNA,
compared with several methods as well as the time, the expense and the time to choose an efficient, simple fast, reasonably priced extraction methods. Finally: Several methods could achieve the quality of molecular biology experiments related requirements, but in the sequence of operation, the time and the price there exist differences.Specific results of this comparison to be possible to provide the basis for the molecular biology experiment selective extraction method.
Applications of Mammalian Somatic Cell Nuclear Transfer
LI Gang;HOU Wen-wen;ZHANG Xiao-rong;ZHANG Yun-hai
2008, 1(4):  48-51. 
Abstract ( 1828 )  
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Somatic cell nuclear transfer (SCNT) provides a new tool for agriculture, medicine and biological basic mechanism research. In cutting-edge researches, SCNT is always coupled with modern biotechnologies such as transgenic techniques, embryonic stem cell protocols to service life science. It is well expected to be helpful to human degenerative diseases cure in near future with the aid of SCNT, although the efficiency is still low, not only in isolation and establishment of embryonic stem cell lines, but also in obtaining viable offspring. The present paper was designed to review the progresses of applications of SCNT in areas mentioned above.
Speices Ideafication and Sequence Analysis of COⅡ,ATPase8 and There tRNA Genes in Grey Francolin
WANG Jing;ZONG Hui;LIU Chou-sheng.ZHANG Li-ping;FAN Wan-hong;WU Jian-ping;YANG Lian
2008, 1(4):  52-56. 
Abstract ( 2028 )  
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Mitochondrial DNA is the extra nuclear germ plasm in eukaryotic cell. It is characterized as simple construction, the evolutionary rate kept relatively
constant, which has been widely applied to the study of systematic evolution, relative relationship and species identification. To acquire the molecular data of species and derived material identification, the phylogenetic information ,validity and sensitivity of the genes were analyzed. Usually, Francolin mitochondrial COⅡATPase8 gene sequences were amplified by PCR, 1138 bp fragment were cloned and sequenced including cytochrome oxidase subunitⅠpartial sequence, tRNASer,tRNAAsp, cytochrome oxidase subunitⅡ, tRNALeu, ATPase subunit 8 complete sequence and ATPase subunit 6 partial sequence.We found that these sequences were proved to have good homology with other 5 species of poultry recorded in The NCBI. The molecular phylogenetic trees based on the sequence of COⅡ,ATPase8 and tRNA from Francolin and other poultry were constructed by NJ method using sofeware MEGA3.1 showing that the relationships are consistent with the morphological and taxonomic results. And COⅡhad more phylogenetic information than the others’ .
Effects of Bovine Flow Cytometrically-sorted Sperm with Two Different Treatments in Vitro Fertilization
JIANG Ming-sheng;LU Yang-qing;HU Chuan-huo;ZHANG Ming;LU Sheng-sheng;LI Lin;LU Ke-huan
2008, 1(4):  56-58. 
Abstract ( 1261 )  
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he objective of this study was to investigate the effect of bovine flow cytometrically-sorted sperm after two treatments. Bovine oocytes were randomly divided into two groups and matured and inseminated in vitro with washing or Percoll treatment. Sperm survival rate and density were recorded following treatments. The results showed that: there was no significant difference in sperm survival and blastocyst rates between the two treatment groups, but washing treatment resulted with higher sperm density compared to percoll treatment; sperm density with washing treatment was significantly higher than that with Percoll treatment (P<0.01). The present studydemonstrated that the efficiency of utilization of bovine flow cytometricallysorted sperm can be improved with washing treatment as it can obtain higher sperm denesity.
The Function of Leptin on Milk
HAN Li-qiang;PANG Kun
2008, 1(4):  59-61. 
Abstract ( 1233 )  
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This paper reviews data on mammary leptin and leptin receptor gene exp
ression as well as on biood and milk leptin levels during the pregnancylactation cycle in humans,rodents and ruminants with the aim of better understanding milk leptin origin and functions.The few published papers report that leptin may be produced by different cell types in the mammary tissue,and may act as a paracrine factor on mammary epithelial cell proliferation.In addition to leptin synthesis,epithelial cells may transfer leptin from the blood,and these two mechanisms may account for the presence of leptin in the milk.If leptin levels in milkare found to be significant,this hormne could be involved in neonate physiology. 
The Application of Disease Resistance Breeding in Livestock and Poultry
LIU Jiang-jun;YUAN Zheng-rong;HE Jun
2008, 1(4):  62-64. 
Abstract ( 1392 )  
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This article expatiates the genetic foundation of disease resistance, present situation and question of method of breeding for disease resistance, and linkages the application of disease resistance breeding of livestock and poultry. At last, prospects the foreground of breeding for disease resistance in the future.
疾病防治
Serological Investigation of Reproductive Disturbance Disease of Intensive Pig Farms in Hubei Province
LI De-xue;SONG Nian-hua;PEI Jie;TIAN Feng;ZHOU Yan-jun;HU Zhi-bin;XIE Jun;ZHANG Hou-zhi;WU Wen-yan
2008, 1(4):  76-79. 
Abstract ( 1273 )  
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The porcine sera from intensive concentrated farms were examined by ELISA、LAT and IHA using the antibodies against seven diseases in 2006, including pseudorabies virus(PRV),porcine reproductive and respiratory syndrome virus(PRRSV),japanese encephalitis virus(JEV),porcine parvovirus(PPV),hog cholera virus(HCV),porcine circovirus(PCV) and Toxoplasmosis. Analysis results indicated that average positive rate of immunity antibody of HC,PR,PRRS,JE,PP were 84.3%,78.3%,84.7%,58.0%,79.7%, separately. The PRRSV infected seriously with the unvaccined pigs' average infective rate of PRRSV was 55.1%, but average infective rate of wildtype PRV was only 17.7%. Coinfectons among PRV,PRRSV and PCV-2 were detected by the 541 serum samples from the 58 infected farms. The results showed that double or triple coinfections were very genenral, which made diseases more complicated and preventions more difficult.
Comparisons of Three Cultivation Conditions Affecting on the Growth of Actinacillus Pleuropeumoniae
ZHAO Ping;LU Zhong-xin;CHU Yue-feng;GAO Peng-cheng;HE Ying;SHI Qin
2008, 1(4):  80-82. 
Abstract ( 1695 )  
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The concertration and the time for logarithmic phase of actinacillus pleuropeumoniae are different due to the difference of cultivationd condition in liquid medium. In this report ,three cultivation methods of stilness, shake and fermentation were used and compared.The data revealed that stillness the highest concertration was 1×109 and time was eighteen hours, shake the highest concertration was 3.7×109 and time was thirteen hours, fermentation the highest concertration was 4.5×109 and time was four hours.Fermentation the highest concertration higher than other two methods and the cultivated process was shortern apparently.Therefore, a foundation was built for producation Actinacillus Pleuropeumoniae antigen massively.
The Research on BCA Reagents Quantitative Protein and FMD Antigen
MIAO Hai-sheng;LI Le;LIAO De-fang;LI Hua-chun
2008, 1(4):  84-86. 
Abstract ( 1060 )  
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Bicinchonininc acid can be used for precisionly quantitative protein.Purified FMDV 146 S antigen with discontinuous gradient sucrose by centrifugation. BCA reagent quantitative 0~200 μg albumin and purified antigen. UV spectrophotometer reading results with 560 nm wavelength. Albumin test results were analyzed using SPSS software,linear analysis and regression analysis were all notable. Calculate the regression equation, Draw a standard curve between protein content and the OD value. Put the data of purified antigen into the regression equation ,to calculate the antigen content.
Compound Pan-grams of Canine Parvovirus Disease on the Efficacy Test
XIAOXiao;;YANG Ji-sheng;LUO Shao-zhong
2008, 1(4):  88-90. 
Abstract ( 1377 )  
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Pan-grams of Compound is a novel compound efficient antibacterial, antiviral drugs can quickly eliminate toxins and animal intestinal endotoxin, enhanced gastrointestinal absorption capability, and enhance immune function. In our national canine parvovirus in dogs is a very common infection of the disease, ulcerative colitis dogs easily aroused and diarrhea. Pan-existing compound of grams on the 30 suffering from the disease in dogs sick canine parvovirus treatment cured 28, died two, the cure rate was 93.3%, treatment yielded a good result.
Effect of Cottonseed Cake and Cottonseed Hull on Incidence ofFinishing Sheep Urolithiasis
LIU Fang;PAN Xiao-liang 
2008, 1(4):  92-94. 
Abstract ( 1264 )  
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In the spring, a finishing sheep field in Xinjiang effluenced urolithiasis caused by feeding excessive cottonseed Hull. Through the etiology and pathology diagnosis,find out the reason is urinary tract infection and formation of struvite stones caused by chronic gossypol poison. After adjusted diet formation, the condition under control.
兽医临床
Clinical Application of Electro-acupuncture in Veterinary
WANG Yu-zhong
2008, 1(4):  107-108. 
Abstract ( 1282 )  
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The article summarized the kinds of veterinary electro-acupuncture apparatus and application of electro-acupuncture in anesthesing and curing the disease of nervous system, digestive system and procreate system. 
Practice of Using Urethane and Sodium Pentobarbital in Rabbits of Surgical Anesthesia
YU Wang-yi;LIU Le-ping;MENG Qiong;YIN Sheng
2008, 1(4):  110-111. 
Abstract ( 1463 )  
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Objective to seek the easy,safe and effective methods of evaluate the effect of combined anesthesia that using Urethane and Sodium Pentobarbital in rabbits of surgical. 32 rabbits which were weighed from 2.0 kg(female and male to be half and half) were divided into four groups by random selection,two groups were used the ear rim vein to give one medicine, two groups were used the abdominal cavity to give other medicine. Anesthesia time of the medical effect results which using the abdominal cavity surpassed the time which using the ear rim vein. Using the different medicine and methods of two kinds of groups haven't the difference for the time of narcotic sleep.
Guoluo Tibetan Sheep in Footrot Disease Etiology and Integ rated Control
ZHANG Jun-liang;QI Zhi-mei
2008, 1(4):  114-115. 
Abstract ( 1761 )  
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Early 2002 to June 2006 in the Guoluo, suffering from disease footrot 2670 etiology Tibetan sheep after an investigation, found mainly caused by the disease causes necrosis bacteria, spirochetes, and other pathogen infection, forage, climate change, grazing, sanitation also affected hoof disease. After injection of procaine penicillin, vitamin B complex, such as copper sulfate taken drug treatment and the relative scientific prevention measures, in addition to the 801 serious incidence of death, in the sheep were recovered and the cure rate to 70%.
畜禽产品
Nutrient Characteristic of the Meat in Linwu Duck
ZHANG Fu-sheng;;LI Li-li;LAN Xin-yi;ZHANG Bin;PAN Ya-fei;CHEN Deng-ke;WANG Jiu-rong;HU Jian-wen;HU Li-xia
2008, 1(4):  121-124. 
Abstract ( 1958 )  
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An experiment was carried out to analyze and evaluate fleshy performance and nutriment ingredients of Linwu duck. The content of nutritive components, amino acids. Inosine monophosphate(IMP) and fatty acids was determined by chemical and instrumental method. The nutritive components of linwu duck were analyzed by chemical and instrumental method. The results showed that the moisture in pectoral muscle was higher than in crural muscle(P<0.05),but the energy in crural muscle was higher than in pectoral muscle(P<0.01), the other indexes of Linwu duck have no significant differences on genders and positions. Seventeen kinds of amino acids, eight kinds of fatty acids and inosinate have no significant differences on gender. Compared with other duck species, Linwu duck has some characteristics which include the lower moisture, the higher contents of IMP ,amino acids and glutamic acid; and also has higher contents of unsaturated fatty acid. 
Biological Characteristics Comparison of Eggs between Goose and Duck
HAN Zhan-bing;HUANG Yan-kun;LIU Jian;FAN Jia-ying
2008, 1(4):  125-126. 
Abstract ( 1411 )  
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In order to discover the differences of eggs between goose ang duck, 56 eggs of Gushi White Goose and 64 eggs of Huainan Ma Duck were involved in this study. The results shown that, every outside and inside characteristics of goose eggs and duck eggs were: egg weight (159.1 g, 62.17g), egg specific density (1.119, 1.094), egg shell thickness ( 0.514 mm, 0.311 mm), Haugh unit (107.77, 86.14), proportion of yolk (38.12%, 30.28%), proportion of egg shell (12.32%, 11.19%), respectively. Egg yolk color of duck is deeper compare with goose, roche color were 11.30 and 4.52 respectively. The rate of blood and meatspot of duck eggs was 12.5%, compare with 3.57% of goose eggs.
动物生产
Effect of IgG from Bovine Colostrum on Serum IgG and Weight Gain in Piglets
HUA Jing;YANG Liu;WAN Shan-xia;ZHAMG Shu-ping
2008, 1(4):  127-128. 
Abstract ( 1317 )  
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120 newborn piglets of Yorkshire were selected in 3 groups randomly. Group A was control and added to physiological saline. Others were added to different amount of IgG in 24 hour to determine the effect of IgG from bovine colostrum on serum IgG of both bovine and piglet and body weight gains in weaning age in 5 and 10 day. The results showed that serum IgG of both bovine and piglet in 5 and 10 day and the body weight grain in weaning age of piglets feeding IgG of 4g were more higher than control group(P<0.05).
A Study on the Effects of Experimental Premix on the Gain and Meat Quality of Fattening Pigs
WANG Xiao-ming;HU Chang-xiong;GHANG Zhi-yong
2008, 1(4):  131-133. 
Abstract ( 1759 )  
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Divided into two groups in random(groups ⅠandⅡ),twenty-four piglets were fed the dietary with 1.5% premix feed from different sources for 105 days in three stages. The results showed that the average daily gain of groupⅠandⅡ was 856 and 867 grams respectively and average ratio of feed to gain was 3.19 and 3.15 respectively.There was no significant differences (P>0.05) between them. However,the group Ⅰhad better performance: meat color, pH, marbling, muscle tenderness, water nolding capatity and intramuscular fat was better than group Ⅱ by 10.00%, 3.68%, 16.67%, 9.38%, 6.71% and 13.07%, which was obviously different from group Ⅱ(P<0.05). Though the average premix cost for one kilo-gram gain was 0.79 and 0.72 yuan respectively. The cost of groupⅠwas rised by 9.70% comparing with group Ⅱ, a pig need much feed cost than groupⅡby 5.6yuan,but this product would have extensive use in furure.
Study on LDH Isozymes Activity in Buffalo Milk and Blood
DENG Yan-mei
2008, 1(4):  134-135. 
Abstract ( 1802 )  
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The relationship between milk producing traits and characteristics of LDH isozymes, including its polymorphisms and relative activity ,were analysed by using PAGE. The result showed that spectrum of the isozymes in buffalo milk demonstrated a similar pattern with blood. The relative activity of LDH1,the highest among all bands of the LDH isozymes, had a significant association with milk yield. And may be used as genetic markers in the buffalo breeding.
经验交流
Exploit and Use Southern Grassland Properly to Accelerate theDevelopment of Stockbreeding
TENG Yong-qing;LI Bin-bin;WANG You-guo;SUN Juan;;YU Yong-xiong;YAN Di
2008, 1(4):  136-140. 
Abstract ( 1671 )  
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The southern part of China has vast area of grassland, but the stockbreeding in there is less developed. The utilization ratio of grassland is also very low. This paper gives a general analysis of the presentday situation of the grassland in southern China as well as the existing problems in the mode of production of stockbreeding. It also rises the idea of establishing new way of stockbreeding; using the grassland properly to increase its output of its, so that “ecology, economy and society” these three elements could develop harmoniously.
Investigation Progress about Animal Reproductive Toxicity of Gossypol
NI Ming;YUAN Hui
2008, 1(4):  144-145. 
Abstract ( 1270 )  
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Gossypol is a noxious substance, has a greater toxicity in animals. This article summarized the investigation progress about animal reproductive toxicity of gossypol. Gossypol can cause spermatogenic cells apoptosis, inhibit production of progesterone by luteum,damage sperm and induce potentia generandi decline.
The Aristolochic Acid A’s Abstraction in Manchurian Dutchmanspipe Stem and Contet Assaying
WANG Dian-ren;WANG Yan-ying;LU Yan;SHEN Hong;ZHANG Zhong-wen;WU Guo-juan
2008, 1(4):  147-149. 
Abstract ( 1479 )  
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Extracted aristolochic con-acid by dehydrate alcohol as the solvent from 150 g manchurian dutchmanspipe stem powder,get crude extract after rotary evaporation and vacuum dehydration,and dissolved extractum with NaHCO3 saturated solution,adjusted the pH to 3 after delamination.Used silica gel G chromatographic colum to laminar analysis. The eluent was made up of chloroform∶acetidin∶methylbenzol(2∶1∶1).Then get flav-crystal 0.389g after recrystallization, identified and assayed the content with R-HPLC. the result showed that the flav-crystal was aristolochic acid A monomer, and the content reached 99.2%. The methold of extraction could get high purity aristolochic acid A,and it was a high performance way.