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20 February 2009, Volume 36 Issue 2
特约综述
Annual Review of Ruminant Nutrition in 2007~2008Ⅲ.Nutrition on Carbohydrate
ANG Jian-ping;WANG Jia-qi;PU Deng-pan;HUO Xiao-kai;GUO Tong-jun;LI Zong-fu
2009, 36(2):  5-13. 
Abstract ( 2111 )  
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〖WT5”HZ〗〓〓Abstract:〖WT5”BZ〗To improve the production efficiency of ruminants, it is important to study nourishment of carbohydrate for ruminal animals because there is above 70% carbohydrate in thier diets. In recent years, the studies of ruminal animal nutrition on carbohydrate focused on the classification of carbohydrate according to the degradation situation of feed in the rumen, the digestion characteristic of starch, the effect of neutral detergent fiber contained and diets components on feed digestion, the effect of feed physical characteristic on the ruminal fermentation, the requirements of carbohydrate for ruminal animals, the development and utilization of carbohydrate feed sources. This article reviewed the classification of carbohydrate in feed, the nutrition of nostructure carbohydrate and structure carbohydrate, the nutrition of carbohydrate relating with physical property, the requirement of carbohydrate for ruminants, the research and development of raw material for cow, beef cattle and other ruminal animals. In the future, there will be more researches focusing on to study the carbohydrate physiological function, action mechanism, regulative mechanism, metabolic pathways and so on by using the molecular biology technology. There will also be various researches on learning the effect of new carbohydrate feed resources feed with ruminal animals.
Annual Review of Ruminant Nutrition in 2007~2008 Ⅳ.Protein and Amino Acid Nutrition
ZHANG Yang-dong;WANG Jia-qi;BU Deng-pan;DONG Xiao-li;WANG Meng;PAN Fa-ming;YANG Guang
2009, 36(2):  14-20. 
Abstract ( 2466 )  
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Protein is the basic nutrition for life. As research advanced how to increase the level of MCP in rumen and improve the digestibility of RUP effectively so as to decrease the nitrogen discharge have been the hot topic. While the mechanism of protein, amino acids and peptide were utilized in rumen, small intestine, large intestine and mammary gland were not clear. Now it is possible for us to study metabolism of protein, amino acids and peptide as the molecular biology, blood vessel intubation and arteriovenous disparity technique used. At the same time, active ingredients in milk have earned much attention in recent years. In this article, the authors have reviewed the metabolism of protein in rumen, small intestine, big intestine, mammary gland and factors those affect their use in gastrointestinal, immunoreactive protein in milk and research outlook.
动物营养与饲料科学
Advances on Feedstuff and Nutrient of Swine
FU Linsheng;XIONG Benhai;GAO Huajie;YI Tao
2009, 36(2):  21-27. 
Abstract ( 2250 )  
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Advances on feedstuff evaluation, nutrient requirements and formulation techniques of Swine are renewed in this paper. It shows that the evaluation parameter for the energy in swine feed is turning to net energy (NE) from digestibility energy (DE) and/or metabolizable energy (ME); the biological value index of amino acids is turning to the standardized ileal digestibility (SID) , which has more practical value compared to the apparent digestibility. The research method of nutrient requirements of swine is turning to the mathematic model calculative method driven by all kinds of parameters which affect the nutrient requirements of swine. The net energy (NE) and the standardized ileal digestibility (SID) have been used as two new indexes in swine nutrient requirements, and the conditions of using the new parameters in the feed formulation design and practical feeding are basically satisfied. Abovementioned research results will provide scientific basis for developing new generation swine feeding and nutrition expert system.

Determination of the Antibacterial Property of Cecropin of and its Characters
WU Xiao-song;LUO Zhen-fu;LI Jun-bo;YANG Dan-dan;HE Jian-hua;FAN Zhi-yong
2009, 36(2):  28-32. 
Abstract ( 1544 )  
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This paper was supposed to study the effects on inhibiting and killing
common pathogenic bacteria of cecropin product by determining its antibacterial
and antibiosis properties. The results indicated that this product had inhibito
ry effects on Escherichiacoli, Salmonelladerby, Stapylococcusaureur, and their m
inimal inhibitory concentrations were respectively 4500,3500,3500 μg/mL; add
itionally, it presented excellent heat resistance and acid resistance. Therefore
, 3500 μg/mL in diets of experimental animal was recommended.

Effects of the Culture of Acrermonium Terricola on Performance and Immunity of Piglets
WEI Jian-zhong;ZHANG Wei;LI Yu;FAN Mei-zhen;WANG Gui-jun;YANG De-kang
2009, 36(2):  33-35. 
Abstract ( 1641 )  
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Thirtytwo DurocLandraceYorkshire piglets of 28dayold were randomly divided into two groups. Control group fed with basal diets and experimental group fed with basal diets supplemented with 0.25% the culture of Acrermonium terricola.The experiment was carried out for 30 d. Compared with the control group the body weight gain of experimental group increased significantly(P<0.05),feed efficiency was improved,the rate of diarrhea decreased distinctly. Level of immune antibody of hog cholera was also increased significantly(P<0.01). These results showed that the culture of Acrermonium terricola could accelerate the body weight gain and improved the immune function of piglets.
生理生化
Review of Physiological Functions and Metabolism Mechanism of Fat
WANG Jianping;;WANG Jiaqi;BU Dengpan;LIU Ning;LI Fadi;LUAN Shaoyu
2009, 36(2):  42-45. 
Abstract ( 2006 )  
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With studying improvement, the fat was learned to be an indispensability nutritive material for animal and human being. It possesses important function of physiology and nutritive metabolize regulating. This article reviewed the physiological function and metabolism mechanism of fat, which included the effect of fat on metabolism, blood and cardiovascular system, immunity function, cell membrane function, brain function and gene express.

The Apoptosis of Mouse Iymphocytes Induced by Ricin Toxin
LIU Linna;;DONG Ying;LI Jiping;LIU Wensen;WAN Jiayu;SUN Yucheng;GAO Hongwei
2009, 36(2):  46-49. 
Abstract ( 1052 )  
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Prepared mouse splenic lymphocytes under aseptic conditions, then treated cells with different concentratio of ricin toxin (0,0.3,3,30,300 μg/mL) and detected its viability at different durations(3,6,12,24 h) by MTS method. The result showed that RT had significant toxic effect on lymphocytes and its viaility only 〖JP2〗60% of control cells when treated by RT for 24 h.〖JP〗〖LM〗〖WT〗〖WT5”BZ〗 In order to invest that if the cell death induced by RT according to the apoptotic pathway, we detected series of typical apoptotic character in the experiment, membrane potential detection, Hoechst33258 staining and DNA ladder. All the results illuminated that RT could induce apoptosis of lymphocytes indubitable. And also, this conclusion can provide parts of theories for the toxic action’s further study of ricin.
Advances in the Research of Runx2
LIN Huixing;ZHOU Zhenlei
2009, 36(2):  53-57. 
Abstract ( 1695 )  
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Runx2 is a member of the Runt homology domain family of transcription factors. As a specific transcription factor for osteocytes, Runx2 plays an important role in the formation and reconstruction of bone tissue. It can determine the differentiation of multipotent stem cells into osteoblastes, promote the matu
rity of chondrocytes and vascularization of cartilage. Runx2 is also associated with the formation of osteoclasts and intracellular matrix, the marrow endothelial cell migration and infiltration, the blood vessel proliferation and the tumor
cell infiltration, and the tooth and skin hairfollicle growth process is also closely connected. Progress in the research of the molecular mechanism regulated by Runx2, a master regulatory gene for activating the osteoblastes genesis, has provided novel insights for the therapeutic strategies of the metabolic diseases of bone.

生物技术
The Isolation Method Establishment of VP1 Gene RNA in Inactivated  Vaccine against FootandMouth Disease
ZHAO Yi;LEI Zhen;WANG Yuhong;LIU Hong;MA Wenge;HUANG Jiong;FU Zihua;YI Zhong;XUE Ying;
2009, 36(2):  58-61. 
Abstract ( 2143 )  
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Inactivated vaccines against foot andmouth disease gathered from different manufacturers were screened by various methods.Gene VP1 RNA in live virus, inactivated vaccine and inactivated virus were isolated, RTPCR amplified, cloned, cDNA sequenced and comparative analysis. The results indicate that,VP1 RNAs isolated from live virus, virus inactivated by BEI and inactivated vaccine emulsified by white oil access to all of the 813 bp specific objectives of fragments, and the expected 813 bp fragment match. Nucleotide sequence revealed that  VP1 homology of the virus was 100% isolated by three methods. BEI and the emulsification process do not damage whole RNA structure of VP1. The isolated method to RNA can be used to analyse sequence of VP1 RNA, which derived from either live or inactivated Footandmouth Disease virus.
Identification and Biological Information Analysis of H5N1 Subtype High  Pathogenic Avian Influenza Virus Isolated from Duck
SUN En-cheng;LIU Chun-guo;HOU Xi-lin;LIU Ming;PIAO Fan-ze
2009, 36(2):  62-67. 
Abstract ( 2140 )  
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A H5N1 subtype avian influenza virus isolated from duck belongs to high pathogenic avian influenza virus. HA and NA gene of the solated strain shares 98.8% and 98.7% nucleotide sequence identity to the HA gene of A/duck/VietNam/Ncvd1/2002(H5N1) and the NA gene of A/duck/VietNam/Ncvd1/2002(H5N1) and A/chicken/Jiangsu/cz1/2002(H5N1), and shares 99. 3% and 98.7% amino acid sequence identity to the HA protein of A/duck/Viet Nam/Ncvd1/2002(H5N1) and the NA protein of A/chicken/Jiangsu/cz1/2002(H5N1). There is several continuous basic amino acid(PQREIRRKKR*G) at the cleavage site of HA protein show its a high pathogenic avian influenza virus, and compared with the other influenza viruses the isolated strain has no difference in Nglycosylation sites. Phylogenetic trees analysis of HA and NA gene suggests that the isolated influenza virus probably originated from the A/duck/Viet Nam/Ncvd1/2002(H5N1).
Screening Microsatellite Primers and their Application for Individual Identification in Faecal DNA from Procapra praprzewalski
HONG Yan-yun;LI Di-qiang;YI Tu-yong;ZHANG Yu-guang;LIU Yi
2009, 36(2):  68-71. 
Abstract ( 1516 )  
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To protect Procapra praprzewalskii appointed as CR (Critically Endangered), twenty pairs of microsatellite primers from Granti’s gazelle and sheep separately were used to identify the individual of przewalskii gazelle through PCR method, we found that 8 loci were polymorphic. The values of gene heterozygosity is 0.71~0.84, the values of effective number of alleles is 3.40~6.59. At the same time, we took advantage of the 8 loci to identify 35 individuals from 39 faecal samples from Procapra praprzewalskii
Establishment of a Loopmediated Isothermal Amplification Assay for the Detection of African Swine Fever Virus
JIANG Yanzeng;ZHU Hongfei
2009, 36(2):  72-74. 
Abstract ( 2351 )  
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A rapid assay for the African swine fever virus (ASFV) by loop mediated isothermal amplification (LAMP) was established. This method was performed in a water bath at 63 ℃ without any other expansive equipment. Positive results were got in 30 min with a sensitivity 100 fold of PCR. And no crossreaction with other usual DNA virus of swine was observed. This method is used suitablely to detect ASFV in field or any other place lack of a laboratory environment.
2009, 36(2):  75-76. 
Abstract ( 1810 )  
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遗传繁育
The Study on Genetic Polymorphism of Eight Microsatellite Loci in Gansu Alpine Fine Wool Sheep that Used for Both Mutton and Wool
CHEN Koukou;YANG Bohui;LANG Xia;GUO Jian;SUN Xiaoping;LIU Jianbin
2009, 36(2):  77-81. 
Abstract ( 1800 )  
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The polymorphism of eight microsatellite loci were analysed in Gansu alpine fine wool sheep that used for both mutton and wool. Allele frequencies, effective number of alleles, heterozygosity and polymorphisms information content were calculated for all microsatellite loci. The results indicated that Ne,H,PIC at BM3501 were the highest(Ne=7.30,H=0.866,PIC=0.848). Ne,H,PIC at BM3413 were the lowest(Ne=2.33,H=0.573,PIC=0.478). There were remarkable polymorphism at eight microsatellite loci in Gansu alpine fine wool sheep that used for both mutton and wool. The eight microsatellite loci were highly genetic polymorphism except BM3413 intermediately genetic polymorphism. Therefore, genetic heterozygosity of Gansu alpine fine wool sheep that used for both mutton and wool was higher, genetic polymorphism was plentiful. There has a vast leeway to be selected. And not only could these eight microsatellite loci be used for genetic polymorphism evaluation in Gansu alpine fine wool sheep that used for both mutton and wool, but also those loci could be applied in further study about wool and meat traits.

Effect of BCB Staining on the IVM of Cow Oocytes
QIAO Limin;;HUANG Chengding;LI Xiangchen;GUAN Weijun; MA Yuehui
2009, 36(2):  82-85. 
Abstract ( 2078 )  
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The aim of the present investigation was to study the effect of IVM of cow with different concentration brilliant cresyl blue (BCB) staining on the oocytes selection. For this purpose, Compact cumulus oocyte complexes (COCs) were recovered from slaughterhousecollected bovine ovaries and classified as control group, which were cultured immediately without exposure to BCB stain;treatment group were stained with different concentrations BCB(13,26,39 and 52 μmol/L BCB) for different time before culture. Treated oocytes were then classified according to their cytoplasm coloration: oocytes with a blue cytoplasm or grown oocytes(BCB+) and oocytes without a blue cytoplasm or growing oocytes(BCB-) . Oocytes were stained with different concentration BCB for different time before culture in this study. The result showed that:①The percentage of Colouring and mature for selected oocytes was different by treated with different concentrations BCB. The percentages of BCB+ oocytes obtained after staining with 26 μmol/L (27.7%),39 μmol/L (27.6%)and 52 μmol/L BCB (31.9%) were higher (P<0.05) than those obtained with 13 μmol/L(2.1%); ②BCB+ oocytes exhibited a higher (P<0.05) maturation rate (mean rate:77.4%;n=62)than BCB(51.3%;n=236) and control oocytes (60.2%;n=98);③There were no significant differences in the percentages of BCB+ oocytes reaching the MII stage among BCB concentrations;④There were no significant differences in nuclear maturation between control and BCB exposed oocytes (BCB+ plus BCB- ),independent of the BCB concentration;⑤After exposure different time BCB, the result showed that the percentage of BCB+ oocytes were different. The percentages of BCB+ oocytes obtained after staining with 26 μmol/L BCB for 90 min(75%) were a higher(P<0.05)than those oocytes obtained after staining with 26 μmol/L BCB for 60 min(57.14%) and 30 min(40%). These results show that the staining of cow cumulusoocyte complexes with BCB before in vitro maturation could be used to select developmentally competent oocytes.
Advances on Related Genes with Delayed Puberty in Mammals
FENG Tao;DI Ran;CHU Mingxing;ZHANG Yingjie;
2009, 36(2):  86-89. 
Abstract ( 1891 )  
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Sexual maturation of humans and mammals was driven by an intermittent discharge of gonadotropinreleasing hormone (GnRH) generated by a network of hypothalamic neurons known as the GnRH pulse generator. Reproductive disorder will present once GnRH release was suppressed or destroyed, which will result in hypothalamic hypogonadism. As for humans, this disorder presents azoospermatism and amenorrhea resulted from hypoevolutismus and sex hypoplasia. Animals affected by hypothalamic hypogonadism present puberty delay and reproductive capacity degression. Mutations of the genes encoding gonadotrophins and their receptors may cause delayed puberty. This review briefly introduced the relationships between GPR54, GnRH/GnRHR, FSH/FSHR, LH/LHR genes and delayed puberty in mammals.
The Chromosome Karyotype Analysis of Pingbian Dawei Mountain Jungle Fowl
CHAI Yan;ZHANG Lichun;LI Lihong;CHEN Baoding;WEN Shengping; LI Qihua;XIONG Baoliang;ZHANG Xi;GE Changrong;JIA Junjing
2009, 36(2):  90-93. 
Abstract ( 2140 )  
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Yunnan Pingbian Dawei mountain jungle fowl were used to study their karyotype by preparing chromosomes with direct bone marrow. It has been shown that the diploid chromosomal numbers were seventy eight(2n=78).The chromosomes were consisted of 10 pairs of macrochmmosomes and 29 pairs of microchromosomes,and the sex chromosomal type was ZZ(♂)/ZW (♀). Based on the measurement of relative lengths,arm ratios and centromeric indexes of the first 5 pairs of chromosomes,the morphology of these chromosomes were described as follows:the pair 1,2 and Z were metacentric,pair 3 was telocentric,pair 4 was submetacentric.
Status Quo and Prospects for the Genetic Manipulation of Dairy Cattle by Biotechnology
XING Xin;PANG Xiaoyang;LIU Guowen;WANG Zhe
2009, 36(2):  94-97. 
Abstract ( 743 )  
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The dairy industry, with regulatory approvals of recombinant chymosin and bovine somatotropin (BST), has been at the forefront of food and agricultural biotechnology. Early applications of genetic manipulation in cattle,may favor production of heterologous proteins in milk for pharmaceutical and medical use. Such applications could benefit genetic modification of milk and milk proteins for food use by providing advance knowledge and experience in mammalian protein expression. Other research opportunity areas that could affect prospects for genetic manipulation of dairy cattle include genome mapping, metabolic pathways, growth and development, and cattle/microbe interactions. In this review, we summary the status quo about them and prospects for the genetic manipulation of dairy cattle in consideration of some difficulties of technology and economy and some restrictions of the cattle genital system.
Analysis of Genetic Structure in Meat Line of Tianshan Snow Goose Using Microsatellite Markers
WANG Zunbao;ZHAO Jie;WANG Wenliang
2009, 36(2):  98-100. 
Abstract ( 1802 )  
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In order to evaluate the line’s breeding intensity and purity, this report using 7 microsatellite loci analyzed the meat line of Tianshan Snow Goose groups’ allele frequency,effective numbers of alleles,mean heterozygosity and the average polymorphism information content (PIC).The results showed that the meat line of Tianshan Snow Goose groups’ PIC was 0.4979, the genomic DNA present neutral polymorphism,and the mean heterozygosity was 0.5367.These indicated this group had highly consistency about their genetic basis,that meaned Tianshan Snow Goose through five generations succesive breeding,the genetic performance tended to stabilization; genomic purity reached a certain level.But it still needs further breeding selection according to the breeding goal.Our experiment selected out three highly polymorphic sites CKW11,CKW14,CKW43,that established the basis of relativity analysis between microsatellites sign and Tianshan Snow Goose groups’ produce performance.
奶业专栏
Thought on Accelerating the Application of TMR Feeding Technique in China
WANG Jing;WANG Jiaqi;BU Dengpan;GUO Weijie;;WEI Hongyang;ZHOU Lingyun;LIU Kailang
2009, 36(2):  101-104. 
Abstract ( 2007 )  
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MR feeding technique can ensure nutrient intake uniform by the cows, save considerable manpower and material resources, and enhance the production performance of dairy cows. Accelerating the application of TMR feeding technique in our country has important practical significance. Smallscale farming is the main mode of feeding dairy cows in our country. Owing to the lack of financial and technical support, most of the small and medium dairy cattle farms can not use TMR feeding technology to improve their cow's production performance. TMR delivery services is a way to produce different stages of TMR feed by specialized companies, and then delivered to neighboring dairy cattle farms. This approach will enable smallscale dairy cattle farms can use the TMR feeding technology, therefore improving dairy cow's production performance, increasing farmer’s income, and promoting the application of TMR feeding technology in our country.
疾病防治
Research on Epidemic Situation of Classical Swine Fever Virus in Swine Farms
HU Jie;;MO Longchun;HUANG Xia;LIU Qi;LU Wenjun
2009, 36(2):  105-106. 
Abstract ( 578 )  
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o investigate epidemic situation of classical swine fever virus (CSFV) in Guangxi, 1626 serum samples of pigs collected from 6 swine farms, were examined by using ELISA technique. The results revealed that 6 pig farms had infected CSFV in Guangxi.The population of positive rate of CSFV in different ages of pigs, such as sow, boar, fattening pigs, weanling piglets,were 7.86% to 29.12%,0% to 23.52%,5% to 22.45% and 8.24% to 18.57%,which averaged at 17.52%,11.83%,15.5% and 12.69% respectively.The serological detection results were correspondence with those of epidemiologic investigation in farms.Most sick pigs showed the clinical symptoms in the type of reproductive syndrome or mild syndrome of CSF in Guangxi.
Research Progress in New Protein PB1F2 of Influenza Virus Type A
WANG Xiaodu; MA Zhiyong
2009, 36(2):  107-108. 
Abstract ( 2096 )  
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Influenza virus type A causes catarrhal rhinitis in human, poultry, porcine and other species. PB1F2 is a new nonstructure protein of influenza virus type A, which is coded by segment 2 of influenza virus. PB1F2 plays important role in accelerating apoptosis of infectious cells as well as the replication and the virulence of virus.
Isolation and Identification of 16S rRNA of Escherichia coli from Chicken〖
SHI Fushan;ZHAO Deming
2009, 36(2):  111-113. 
Abstract ( 1692 )  
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Feces samples were collected from sick and dead chicken likely caused by Escherichina coli in a chicken farm,thepathogens were isolated and biochemically identificated, 6 bacterium were isolated from 8 feces samples. According to the highly conservative nature of bacterial 16S rRNA gene, a set of primers with broad range for Escherichina coli was designed,and 1 strain of bacteria was detected by polymerase chain reaction (PCR) and compared with 16S rRNA sequence of Escherichina coli from GenBank.The resembling rate can exceed 99%.The established PCR method is specific ,simple , easy, and available to identify Escherichina coli.
2009, 36(2):  118-118. 
Abstract ( 1376 )  
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Studies on the Rule of Shedding of Giardian cysts in Puppies after Artificial Infection
ZHAO Yongjun;WEI Yanhui;LIU Jianqiang
2009, 36(2):  125-126. 
Abstract ( 1424 )  
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Faecal samples of puppies after artificial infection were examined for Giardia cysts by zinc sulphate floatation method.The result indicated that the rule of Giardia canis cysts in puppies was periodism.Giardia cysts were detected in the 8th days after artificial infection.Cysts shedding peaked in 18 days.The peak of cysts shedding continued 1~2 days.The next cysts shedding peaked in 2~3 days.But all of the peak of cysts shedding continued no more than 2 days.

流行病防治
Research Progeress on the Epidemilolgy of Nipah Virus
XIE Jinwen;;LV Sufang;;SHEN Xu;SHEN Zhiqiang
2009, 36(2):  128-129. 
Abstract ( 1481 )  
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Nipah virus disease is a novel spirited zoonosis caused by Nipah virus of paramyxovirus, which induced critical harm to human and animals including swine.The etiological characteristics,epidemiology of Nicpah virus and clinical symptom of Nipah virus disease were reviewed in this article.
Review of Prion Disease of Human
ZHANG Taixiang;TIAN Guoning;ZHANG Jinling;TIAN Guohua
2009, 36(2):  130-132. 
Abstract ( 1398 )  
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In this paper, the research of pathogeny, clinic symptom, diagnose technique and treatment of the prion disease of humans were reviewed, such as CJD and vCJD. It is important to prevent and control these diseases.
兽医临床
The Effect of Areca Catechu on Tapeworm in Dog
XIAO Xiao;XIAO Yan;SHEN Xuewen; WANG Xiaojian
2009, 36(2):  135-137. 
Abstract ( 1509 )  
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The aim of this paper was to test the effect of areca catechu to tapeworm in dog, definite the effective dose, and benefit procedure. The results showed that lowdose had a certain effect of the insecticide, which easily made tapeworm tolerant to the drug and had poor effect. Because of the toxicity of areca catechu, the clinical side effects for a long time and other factors, the best usage should be 0.3 g/kg per kilo of body weight, over every three to four days for one week as a treatment course.
Organizational Structure Observation of the Lung of Somatic Nuclear Transfer Cattle
WANG Xiaoli;WEI Jingwei;FANG Huiling;MENG Fanli;XIN Lijiang;XIANG Changjian;SHI Deshun
2009, 36(2):  138-139. 
Abstract ( 1909 )  
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The lung of somatic nuclear transfer cattle was observed by optical microscopy and HE staining techniques in this paper. The basic structure of the lungs of the somatic nuclear transfer cattle was similar with the basic structure of the lung of the natural breeding cattle, including the bronchi, the bronchioles, terminal bronchiole, respiratory bronchioles and alveolar duct, the alveolar sacs and alveolar. But the structure of somatic cell cloned cattle lung was faintly, the wrinkle of the bronchiole was disappear, the main structure of its mucosal was the cubic epithelial. The alveolar was in a halffull capacity, and the alveolar space was small, the alveolar septal was thicker than normal cattle with containing collagen and elastic fibers. The cell structure of alveolar was unclear, and alveolar was full of contents because of disease.
Study on the Treatment of Umbilical Hernia of Piglet
WANG Bin
2009, 36(2):  142-143. 
Abstract ( 1496 )  
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The objective of this study is to explore the simple and effective method to prevent, diagnose and treat piglets umbilical hernia. Through analysis of case information of nine piglets, theirs umbilical hernia were cured successfully by conservative treatment. Compared with other conservative and surgical treatments, the advantages and disadvantages of various methods and indication were samarized. The results showed that congenital umbilical hernia was closely related to the method of cutting umbilical cord and genetic,and root cause of acquired umbilical was exorbitant abdominal pressure.Conservative treatment should be given preferentially in treatment of recoverable hernia. Unrecoverable hernia can be treated by surgery. The preventive measures of this disease is eliminating harmful related genes, standardizing methods of cutting umbilicalcord and decreasing the factors that increased abdominal pressure in the process of feeding management. 
动物生产
Comparison on Growth Curve Fitting of White Muscovy
ZHANG Haibo;DUAN Xiujun;ZHANG Yiyu;;XU Qi;LIAO Jing; WANG Cunbo;CHEN Guohong
2009, 36(2):  148-151. 
Abstract ( 2198 )  
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he early bodyweight of White Muscovy with different gender were measured, and three nonlinear curves of Logistic, Von Bertalanffy and Gompertz model were used to fit the growth curve in this study. The results showed that White Muscovy grown slowly before the age of 4 weeks, but the male Muscovy showed higher growth rate than female duck after this stage. The Gompertz model generated the best effect concerned with goodness of fit(R2), residual sum of squares(E), and suitability of χ2 test. Further more, the fitting parameters of Gompertz model between genders were compared, the male Muscovy showed higher initial weight, mature weight, largest weight gain per week and inflection point weight and lower initial growth rate than female Muscovy . The inflection age of male Muscovy was 7.03 weeks and female Muscovy was 5.37 weeks, respectively. The relative growth rate of male Muscovy was lower than female before 4 weeks but higher after 4 weeks and the difference of absolute growth rate was high between genders.
Effects of Age and Sex on Slaughter Characteristics and Meat Quality of Poll Dorset×Small Tail Han Sheep F1 Lamb
WANG Menglin;LUO Qiujiang;YANG Kailun;TANG Zhigao;ZHU Wenyuan;MEN Xiaoming
2009, 36(2):  152-155. 
Abstract ( 2055 )  
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The effects of slaughter age and sex on lamb meat performance and quality of Poll Dorset × Small Tail Han Sheep F1 were studied. Five male lambs, 5 female lambs of 7monthold and 5 male lambs of 13monthold, fed under the same feeding conditions, were slaughtered to study slaughter characteristics and meat quality. The live weight and carcass weight of the 13monthold lamb were higher 41.85%(P<0.01)and 56.51%(P<0.01)than that of the 7monthold respectively. The dressing percentage of 13monthold lamb was significantly higher than 7 months (P<0.05), it showed that 13monthold lamb on slaughter characteristics was higher very markedly than 7monthold. The lean meat weight, eye muscle area and meatbone ratio were higher 30.40% (P<0.01), 30.04% (P<0.01) and 15.56%(P<0.05) than that of the 7monthold respectively. The GR value and fat weight of 13monthold were higher 117.23% (P<0.01) and 263.40% (P<0.01) than 7monthold lamb. The shear force value, drip loss, collagen content and muscle fiber diameter of 13monthold were significantly higher than 7monthold Lamb (P<0.05). The live and carcass weight of male lamb were higher markedly than female (P<0.05), but the fat percentage of female was significantly higher 11.96% (P<0.05) and 14.36% (P<0.05) than that of male. Results showed that slaughter age markedly affected slaughter characteristics and meat quality of lamb, and sex affected slaughter characteristics of lamb. 
Analysis of the Reasons for Rejection of Puppies in a Police Dog Breeding Center
LIU Hu;GU Xuhua;WANG Lin;MA Dajun
2009, 36(2):  156-158. 
Abstract ( 672 )  
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The training acceptance rate and reasons for rejection of 2284 puppies breeding between 2000 and 2007 in one police dog breeding center were analyzed. The results indicated: The qualification rate of 2monthold puppies is 99.12%; the training acceptance rate, rejection rate and mortality rate of 2~6 months old puppies are 87.40%, 9.16% and 3.44% respectively, and the main reasons for rejection are lacking retrieval drive and fearfulness, accounted for 60.11% and 16.85% respectively. Suggesting that retrieval drive and fearfulness are important traits in both dog breeding and puppy training program.

Effect of Light on Puberty of Lambs in Nonbreeding Season
GUO Tingting;LIU Yueqin;ZHANG Yingjie;WANG Xin
2009, 36(2):  159-161. 
Abstract ( 1390 )  
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Mongolia lambs were studied in nonbreeding season in this experiment, 24 six month old and healthy Mongolia lambs with similar body weight were divided into two groups: lambs of experimental group were provided with long light (16 h light∶8 h dark) primarily then provided with short light (8 h light∶16 h dark), lambs of control group were provided with natural daily light, in order to study the effect of light on puberty of lamb in nonbreeding season. The result showed that the body gain of the experimental group was remarkable lower than the control group’s during 0~15 d(P<0.05), and there was no difference between two groups in other time(P>0.05). The oestrus rate of the experimental group(91.7%)was higher than the control group’s(16.7%). The average number of ovulated follicles were 1.2 in the experimental group, which was higher than the number in the control group. Light control can make lamb ovulate and have oestrus behavior in the nonbreeding season.
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Effect of Clomifene Citrate with Differ Dosage on Laying Performance of Silky Fowl Hens
HUANG Yankun;HAN Zhanbing;WANG Changan;LIU Shuai;LIU Jian
2009, 36(2):  162-163. 
Abstract ( 2161 )  
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The study was conducted to investigate the effect of clomifene citrate dosage on laying performance of silky fowl hens. 120 birds were allocateded to 4 treament groups randomly. The clomifene citrate was added to diet at 12∶00 PM every day after grind. The birds were supplement with clomifene citrate at 0, 15, 20, 25 mg/day. The clomifene citrate was used two courses of treatment continually, each persisting 2 d and then 5 d suspend. Results showed that there was not difference among 4 groups in laying rate and hatchableegg rate silky fowl hens, but broodinghens were decreased in 3 trial groups. 