Abstract: This review summarized the research progress of feed additives in rumen fermentation in 2009. Ionophores, such as monensin alters volatile fatty acid (VFA) production in the rumen towards more propionate (Pr) production at the expense of acetate (Ac), which results in increased glucose availability to the animal through gluconeogenesis, decreases the production of methane by inhibiting H2-producing bacteria but not directly inhibiting methanogens. Ionophores decrease milk fat content by an incomplete biohydrogenation process of long-chain fatty acids in the rumen that interferes with de novo fat synthesis in the mammary gland. Adding fibrolytic enzymes and amylase to ruminants can improve the nutrient digestibility. Saponin decreases the production of methane by inhibiting rumen ciliate protozoa. The appearance and proliferation of tannin-tolerant bacterial species to enhance their tolerance to tannins and only condensed tannins extract influence the ruminal biohydrogenation. Essential oils reduce protein and starch degradation and an inhibition of amino acid degradation in rumen due to the selective action on certain rumen micro-organisms. DFM include yeast, lactic acid bacteria, Bacillus can increase in nutrient digestibility, alteration of the proportion of volatile fatty acids produced in the rumen, reduction in ruminal ammonia, and increase of ruminal microorganism population. But the beneficial effects of yeast products depend on lactation stage, diet characteristic and environmental condition.

Abstract：18 Chinese Holstein cows of 2 to 4 parity at mid-lactation were selected,randomly divided into 3 groups of six cows, group 1 and group 2 were added to each of 5% and 15% proportion of glutamate thalli protein, the control group does not add glutamate thalli protein in order to assess the influence on production performance of dairy cows. The results showed that 5% or 15% glutamate thalli protein was used in place of partial protein feedstuffs of dairy cows in summere，roughage intake was promote increased, and milk production was decreased slowly, but milk quality was not influenced significantly. 

Abstract: Study on the effects of Cu(Ⅱ)-exchanged montmorillonite nanoparticles on growth performance and some blood biochemical indexes of pigs.A total of 72 weaning pigs (Duroc×Landrace×Yorkshire) were used to investigate the effects of Cu(Ⅱ)-exchanged montmorillonite nanoparticles(MMT-Cu) on growth performance and some blood biochemical indexes of these pigs and verify MMT-Cu can promote growth performance more than antibiotics.The weaning pigs were allocated to three dietary treatments in a randomized complete block design.The dietary treatments were basal diet，basal diet + 20 mg/kg tylosin+100 mg/kg SM2 and basal diet + 1500 mg/kg MMT-Cu.The results showed that the addition of MMT-Cu to the diet decreased extremely significantly（P<0.01）the F/G during the whole test; the concentration of ALB,SUN,Ch,Tp in surum decreased extremely significantly （P<0.01）; the concentration of Ca,GPT in serum increased extremely significantly (P<0.01) and TG,GLU decreased(P<0.05). These pigs feed the basal diet + 1500 mg/kg MMT-Cu increased the ratio of F/G，and there were various effects on ALB,Ca,GPT,SUN,TG,Ch,TP,GLU.

Abstract: Experiment was designed to culture pulmonary artery smooth muscle cells of broiler with the enzymaic digestion method.Pulmonary artery media layer from 1 to 7 day old broilers was cut into less than 1 mm×1 mm pieces， and was digested for pulmonary artery smooth muscle cells by 0.1％ collagenase type Ⅱ. The obtained cells were then cultured and subcultured. Identifications of the cells were performed with the morphological feature and immunohistochemical stain using monoclonal antibody against smooth muscle αaction.The results showed that live cells and HE stained cells were typical spindleshaped and overlapping layer form of vascular smooth muscle cells under phase contract and light microscope. αactin positive stained cells were more than 97％.Therefore，the results showed that 0.1％ collagenase type Ⅱcould digest and isolate broiler PASMC. This method for culture of PASMC has advantages of easy performance, more living cells and short period for passaging．This method can be used for study in cadiovascular disease such as pulmonary hypentension and pulmonary vascular structural remodelling．

Abstract: This experiment was carried out to study effects of follicle size on collecting effect and developmental competence of goats oocytes derived from abattoir ovaries following maturation, fertilization and culture in vitro. In this experiment, the aspiration method was applied to collect the follicular oocytes. The results showed that follicle size directly affected collecting effect of oocytes and development of oocytes after IVMFC. The rate of collecting oocytes, the avaible oocyte and maturation was interrelated with the diameter of follicular positively. Both fertilization and cleavage rates of oocytes from 2.1 to 5 mm follicles were significantly higher than from >5 mm follicles and ≤2 mm follicles, which were suitable for embryo in vitro production. The oocytes from ≤2 mm follicles had the lowest developmental competence in vitro. Both fertilization and cleavage rates of oocytes from >5 mm follicles decreased ascribed to oocytes degeneration. We could improve the developmental competence of oocytes from >5 mm follicles by decreasing the cultural time in vitro.

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Abstract: Establishing a method of PMCA for 22L strain PrPSc based on the method called protein misfolding cyclic amplification (PMCA) established by Saborio and Soto to research the biochemical mechanism of PrPSc formation. Under the existence of the anion detergent lauryl sodium sulfate (SDS), the method can directly degrade the products that producted by repeated amplification, and can in the brain tissue homogenate of hamster effectively imitate the replication mode that PrPSc run in vivo in a accelerated way. Therefore, it can amplify PrPScto high concentrations in a few hours. We found that in vitro unbolted brain tissue homogenate is more efficiently than purified reconstituted about PrPSc conversion, this discovery implied that highly efficient PrPSc conversion may associated with other unknown host factor. Researching indicated that the improved PMCA technique that we established can be used to research the biochemical mechanism of PrPSc formation.

Abstract: Probiotics is an organic and healthy additives to apply in food and feed，there are a lot of interesting for probiotics，and it was studied extensively. We could know from the recent study that the healthy characteristics was revealed gradually. The writer reviewed some published datas in public，summarized simply the quality of probiotics，such as the suppressed to some bowel inflammation，absorbed to nutrients，degraded the cholesterol，restrained to colon cancer and species- specificity in application. At the same time，analysed briefly the development in the future.

Abstract：Faecal samples of puppies after artificial trophozoite infection were examined for Giardia cysts by zinc sulphate floatation. The results indicated that trophozoite could infect puppies and the shedding of Giardia canis cysts in puppies was intermittent. The period of intermittence was 7 to 8 days. Giardia cysts were detected after 3 days，cysts shedding peaked in 13 days after inoculation ，and lasting for 3 to 4 days. In the intermittent period，we also detected a little amount Giardia cysts everyday. The infected puppies had the symptoms of diarrhea and depressed and weight loss.