The purpose of this experiment was probing the effect of multienzyme in the miscellaneous meal diet and prepara-〖WT〗〖HT〗〖HJ〗〖LM〗

tions on growth, metabolism and the blood indices in weanling piglets.48 weanling piglets was used and divided into 2 groups. 1% multienzyme was added in the experimental group on the basic of control group. The experiment was divided into two stages, earlier stage (1 to 21 d) and later stage (22 to 50 d). The results showed that experimental group was better than control group in the daily gain, the feed to gain ratio(F/G) and the incidence of diarrhea in 1 to 21 d （P<0.05）,and in full distance test (1 to 50 d) experimental group was better than control group in the daily gain, F/G and the incidence of diarrhea, but only F/G was different markedly （P<0.05）.In 21 d the digestibility of all the nutrients of experimental group were better than those of control group (P<0.05) except crude ash. In 50 d the digestibility of crude fat and crude fibre of experimental group were better than those of control group (P<0.05), and the others had no difference. The results of blood biochemical indexes showed that in 21 d and 50 d the experimental group were all better than those of the control group in TP and ALB, but had no obvious difference; the experimental group was lower than that of control group in BUN and only had obvious difference in 21 d (P<0.05); the experimental group was higher than that of control group in GLU and only had obvious difference in 21 d (P<0.05).

To study the expression of the duck hepatitis virus 1(DHV1) VP1 gene in E.coli ,one outer primer pair and one inter primer pair containing EcoR Ⅰ and Sal Ⅰ according to complete genome of DHV1 were designed. PCR products were re cycled by outer and inter primer pair .The PCR products and pET-32a vector were digested by EcoR Ⅰ and Sal Ⅰ to construct the recombinant plasmids.Then the recombinant plasmids were transfected into E.coli BL21 and Rosseta. After digestion, PCR and sequencing the target gene were insert expression vector correctly. With different concentrations of IPTG induced bacteria express VP1 gene, the bacterias were collected and examined by SDS-PAGE.The results showed that the VP1 gene fragments hard to express in E.coli Rosseta and BL21.

yometra is a common disease of canine. The majority of canine pyometra incidence was observed in metoestrus.It can be classified as open-cervix and closed-cervix. Pyometra is a uterine inflammatory disorder characterized by cystic endometrial hyperplasia, the increasing of progesterone and the release of estrogen, and by ascending uterine infection with vaginal bacteria. In this article, the etiology, diagnosis and treatment of canine pyometra were reviewed.