牛源大肠杆菌质粒介导喹诺酮类耐药基因的检测分析

doi:10.16431/j.cnki.1671-7236.2019.08.033

Abstract

Abstract:

In order to understand the gene distribution of plasmid-mediated quinolone resistance and its resistance to quinolone antibiotics in bovine E.coli in Xinjiang in recent years.116 strains of E.coli were isolated from 12 large-scale dairy farms in Shihezi, Shawan, Kuitun, Manas and Yili of Xinjiang in 2016-2018.The PMQR resistance gene was amplified by PCR and its resistance was detected by drug sensitivity test.The results showed that 62.93% of the 116 bovine derived E.coli strains were resistant to ampicillin, which was the strongest among the 15 tested antibiotics followed by streptomycin, tetracycline, kanamycin and enrofloxacin, whose resistance rates were 56.90%, 54.31%, 43.10% and 42.24%, respectively.The resistance rates to ceftazidime and cefotaxime were lower (7.76% and 11.21%).The PMQR resistance genes carried by the E.coli in Xinjiang were mainly qnrA, qnrS and aac (6')-Ⅰb-cr.The PCR results showed that there were 31 strains carrying PMQR resistance genes in 116 strains, and the positive rate was 26.72%.26, 4 and 1 strains carried 1, 2 and 3 kinds of PMQR resistance gene, accounting for 22.41%, 3.45% and 0.86% of all strains, respectively.In conclusion, the PMQR resistance genes mediated by the E.coli in Xinjiang were mainly qnrA, qnrS and aac (6')-Ⅰb-cr, and it was common for the E.coli resistance to enrofloxacin, norfloxacin, ciprofloxacin and levofloxacin in this area.

Key words: Xinjiang; bovine Escherichia coli; PMQR resistance genes; drug susceptibility test

CLC Number:

S852.61

YANG Yanli, ZHANG Xingxing, HUANG Xin, WU Tongzhong, HE Yanhua, HAN Mengli, ZHONG Fagang. Analysis and Detection of Plasmid-mediated Quinolone Resistance Genes in Bovine Escherichia coli[J]. , 2019, 46(8): 2462-2469.

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Analysis and Detection of Plasmid-mediated Quinolone Resistance Genes in Bovine Escherichia coli

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