神经肽S及其受体对伪狂犬病病毒感染的体外作用研究

doi:10.16431/j.cnki.1671-7236.2019.08.031

Abstract

Abstract:

To explore whether NPS and NPSR were involved in the process of pseudorabies virus (PRV) infection, the expression profiles of NPS and NPSR, virus gene and the related cytokines during PRV infection in vitro were studied by cell culture, RT-PCR, Real-time PCR and shRNA methods.The results of RT-PCR and Real-time PCR showed that the expression of NPS and NPSR in mouse embryonic fibroblasts (3T3 cells) infected with PRV increased extremely significantly 12 and 18 h after PRV infection (P<0.01).After stably interfering with the expression of NPSR in 3T3 cells by shRNA technology, the expression of PRV gE gene decreased extremely significantly (P<0.01).Adding exogenous NPS could significantly enhance the expression of PRV gE gene and cytokines IL-6 and TNF-α in 3T3 cells infected with PRV.However, the expression of PRV gE gene and cytokines IL-6 and TNF-α were extremely significantly inhibited by adding NPSR inhibitors (P<0.01), which was contrary to the effect of NPS.These results indicated that the robust expression of NPS and NPSR could effectively inhibit the replication of PRV in 3T3 cells, which indicated that NPSR was an important factor for the effective infection of PRV in 3T3 cells.Exogenous NPS could aggravate the inflammatory response induced by PRV infection by regulating the expression of inflammatory cytokines.These results provided a basis for further exploring the mechanism of NPS and NPSR involving in PRV infection in vivo.

Key words: neuropeptide S (NPS); NPSR; pseudorabies virus (PRV); 3T3 cell

CLC Number:

R914

CAI Zifeng, TANG Mengyao, HUANG Huipeng, CHEN Jilong, QI Baomin, YANG Guihong. Study on in vitro Efficacy of NPS and NPSR on Pseudorabies Virus Infection[J]. , 2019, 46(8): 2446-2454.

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Study on in vitro Efficacy of NPS and NPSR on Pseudorabies Virus Infection

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