皱皮香猪WIF1基因结构变异WIF1-I8-sv889的研究

doi:10.16431/j.cnki.1671-7236.2019.08.028

Abstract

Abstract:

To explore the relationship of the structure variation of WIF1-I8-sv889 in the WIF1 (Wnt inhibitory factor 1) gene and the feature of body hair formation, the histological morphology of hair follicle was observed by using the frozen section technology and histochemistry dyeing method.The frequency of WIF1-I8-sv889 locus was determined by PCR method, and the functional elements in structural variation regions were analyzed by the online software UCSC and RegRNA 2.0.Histological research showed that compared with the skin structures of Xiang pig and Large White pigs, Xiang pig with wrinkled skin hair follicles appeared hyperplasia, hair root sheath extends into the dermis, forming spinous processes, and in Xiang pig with wrinkled skin, there were more hair follicles in the wrinkled depression, but the hair follicles in the wrinkled convex part were relatively less;Statistics on the width of the hair bulb and the number of hair follicles, the width of hair bulbs significantly increased in the hair follicles of Xiang pig with wrinkled skin (P<0.05), the number of hair shafts in a single hair follicle extremely significantly increased (P<0.01).Specific primers were designed at both ends of the WIF1-I8-sv889 structural variation breakpoint, the amplified fragment long was 1 383 bp, compared with the reference genome, 889 bp in 1 383 bp was the SV interval, 581 bp deleted, and 308 bp inverted.The population distribution results showed that, the polymorphisms of WIF1-I8-sv889 locus was abundant in the pig groups, there presented three genotypes, including types of Ⅱ, DI and DD;No genotype Ⅱ was detected in the Xiang pig with wrinkled skin;Compared with the common Xiang pig and Large White pigs, the D allele was dominant in the Xiang pigs with wrinkled skin (94.44%);Its frequency was significantly or extremely significantly higher than that of the other two pig groups (P<0.05;P<0.01).The results suggested that the structural variation of WIF1-I8-sv889 of WIF1 gene might be related to the morphogenesis of hair follicles in Xiang pig with wrinkled skin.

Key words: Xiang pig with wrinkled skin; hair follicle; structural variation; WIF1 gene

CLC Number:

S828

LIU Xiaoli, RAN Xueqin, LIU Chang, NIU Xi, HUANG Shihui, WANG Jiafu. Study on Structural Variation WIF1-I8-sv889 of WIF1 Gene in Xiang Pig with Wrinkled Skin[J]. , 2019, 46(8): 2423-2430.

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Study on Structural Variation WIF1-I8-sv889 of WIF1 Gene in Xiang Pig with Wrinkled Skin

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