黔北麻羊Tβ4基因多态性及生物信息学分析

doi:10.16431/j.cnki.1671-7236.2019.08.026

Abstract

Abstract:

This study was aimed to screen the polymorphism of thymosin beta 4 (Tβ4) gene in Qianbei Ma goat, and analyze the genetic structure by bioinformatic method.Primers were designed according to predicted sequence of Capra hircus Tβ4 gene in GenBank (accession No.:NC_030810 and NW_017189516) by Primer Premier 5.0.The mixed DNA pool and PCR products combined with direct sequencing were used to obtain the polymorphisms of exons 1, 2 and 3 in chromosome X and exon 1 in chromosome 3 of Tβ4 gene.The homology, phylogenetic tree, physicochemical properties, hydrophobicity, coiled coil, transmembrane structure, signal peptide, subcelluar localization, structure domain, phosphorylation site, glycosylation site, secondary structure and tertiary structure of Tβ4 gene in Qianbei Ma goat were analyzed using bioinformatic analysis softwares.The results showed that a total of 10 SNPs (G55C, C82T, G112A, C129T, G216A, G431A, G551T, T579A, A609G and A619G) were screened.The homology analysis results showed that the identity of Tβ4 gene between Qianbei Ma goat and Bos taurus, Equus caballus, Sus scrofa, Homo sapiens, Heterocephalus glaber, Rattus norvegicus, Mus musculus and Gallus gallus were 97.8%, 96.3%, 95.6%, 94.1%, 94.8%, 91.1%, 92.6% and 86.7%, respectively.Protein physical and chemical properties analysis showed that the theoretical isoelectric point of Tβ4 protein was about 5.02, the minimum value of hydrophilicity was -3.011, no hydrophobic amino acid and coiled coil existed, which was not a transmembrane protein and no signal peptide existed, was located in the nucleus (60.9%), mitochondria (4.3%), cytoskeleton (17.4%) and cytoplasm (17.4%).There was 1 THY domain, 1 threonine phosphorylation site, no N-glycosylation site and 4 O-glycosylation sites.The analysis of secondary structure showed that C129T mutation in Qianbei Ma goat made the increase of α-helix and the decrease of random coil.This results indicated that the polymorphism of Tβ4 gene in Qianbei Ma goat was successfully screened, which provided a theoretical foundation for the study of molecular genetic marker-assisted selection in breeding of Qianbei Ma goat.

Key words: Qianbei Ma goat; Tβ4 gene; bioinformatics

CLC Number:

S852.65⁺9.1

LUO Zheng, WENG Jimei, AI Jinxin, LONG Anju, LUO Weixing, CAI Huifen. Polymorphism and Bioinformatics Analysis of Tβ4 Gene in Qianbei Ma Goat[J]. , 2019, 46(8): 2406-2414.

References 25

[1]	GOLDSTEIN A L,SLATER F D,WHITE A.Preparation,assay and partial purification of a thymic lymphocytopoietic factor (thymosin)[J]. Proceedings of the National Academy of Sciences of the United States of America,1966,56(3):1010-1017.
[2]	HUFF T,CHRISTIAN S G,OTTO A M,et al.β thymosins,small acidic peptides with multiple functions[J]. The International Journal of Biochemistry & Cell Biology,2001,33:205-220.
[3]	LOW T L,HU S K,GOLDSTEIN A L,et al.Complete amino acid sequence of bovine thymosin β4:A thymic hormone that induces terminal deoxynucleotidyl transferase activity in thymocyte populations[J]. Proceedings of the National Academy of Sciences of the United States of America,1981,78(2):1162-1166.
[4]	ZIEGLER T,KRAUS M,HUSADA W,et al.Steerable induction of the thymosin β4/MRTF-A pathway via AAV-based overexpression induces therapeutic neovascularization[J]. Human Gene Therapy,2017,Epub ahead of print.
[5]	KIM S,KWON J.Thymosin β4 has a major role in dermal burn wound healing that involves actin cytoskeletal remodelling via heat-shock protein 70[J]. Journal of Tissue Engineering & Regenerative Medicine,2017,11(4):1262-1273.
[6]	GAO X Y,HOU F,ZHANG Z P,et al.Role of thymosin beta 4 in hair growth[J]. Molecular Genetics and Genomics,2016,291(4):1639-1646.
[7]	YUAN J,SHEN Y,YANG X,et al.Thymosin β4 alleviates renal fibrosis and tubular cell apoptosis through TGF-β pathway inhibition in UUO rat models[J]. BMC Nephrology,2017,18(1):314.
[8]	ZHENG X Y,LV Y F,LI S,et al.Recombinant adeno-associated virus carrying thymosin β4 suppresses experimental colitis in mice[J]. World Journal of Gastroenterology,2017,23(2):242-255.
[9]	SAHIN S,EKINCI O,SECKIN S,et al.Thymosin beta-4 overexpression correlates with high-risk groups in gastric gastrointestinal stromal tumors:A retrospective analysis by immunohistochemistry[J]. Pathology Research and Practice, 2017,213(9):1139-1143.
[10]	RUCHI S,KARINA R G,MARCOS R.Thymosin β4 inhibits PDGF-BB induced activation,proliferation,and migration of human hepatic stellate cells via its actin-binding domain[J]. Expert Opinion on Biological Therapy,2018,18(sup1):177-184.
[11]	WANG L,CHOPP M,SZALAD A,et al.Angiopoietin-1/Tie2 signaling pathway contributes to the therapeutic effect of thymosin β4 on diabetic peripheral neuropathy[J]. Neuroscience Research,2018,Epub ahead of print.
[12]	LEVER M,THEISS C,MOROSAN-PUOPOLO G,et al.Thymosin β4 overexpression regulates neuron production and spatial distribution in the developing avian optic tectum[J]. Histochemistry and Cell Biology,2017,147(5):555-564.
[13]	HAO Q,HE L,ZHOU J,et al.A dimeric thymosin beta 4 with novel bio-activity protects post-ischemic cardiac function by accelerating vascular endothelial cell proliferation[J]. International Journal of Cardiology,2018,261:146-154.
[14]	SKRUBER K,READ T A,VITRIOL E A.Reconsidering an active role for G-actin in cytoskeletal regulation[J]. Journal of Cell Science,2018,131(1):jcs203760.
[15]	SHAH R,REYES-GORDILLO K,CHENG Y,et al.Thymosin β4 prevents oxidative stress,inflammation,and fibrosis in ethanol-and LPS-induced liver injury in mice[J]. Oxidative Medicine & Cellular Longevity,2018,2018:9630175.
[16]	JIANG Y,HAN T,ZHANG Z G,et al.Serum thymosin beta 4 as a noninvasive biomarker in patients with nonalcoholic steatohepatitis[J]. Revista Espanola de Enfermedades Digestivas,2017,110(1):19-24.
[17]	罗卫星,史忠辉,刘贞德,等.黔北麻羊种质特性研究初报[J].山地农业生物学报,2010,29(5):466-470. LUO W X,SHI Z H,LIU Z D,et al.The preliminary investigation in characteristics of Qianbei Ma goat[J]. Journal of Mountain Agriculture and Biology,2010,29(5):466-470.(in Chinese)
[18]	罗卫星,张启林,穆林,等.黔北麻羊的肉用性能和肉质特性研究[J].西南农业学报,2010,23(5):1706-1710. LUO W X,ZHANG Q L,MU L,et al.Study on mutton performance and quality of Qianbei Ma goat[J]. Southwest China Journal of Agricultural Sciences,2010,23(5):1706-1710.(in Chinese)
[19]	ZHAO Y,SONG J,BI X,et al.Thymosin β4 promotes endothelial progenitor cell angiogenesis via a vascular endothelial growth factor dependent mechanism[J]. Molecular Medicine Reports,2018,18(2):2314-2320.
[20]	WANG X,YANG G,LI S,et al.The Escherichia coli-derived thymosin β4 concatemer promotescell proliferation and healing wound in mice[J]. Biomed Research International,2013,2013:241721.
[21]	JANARTHⅡNI R,WANG X,CHEN L,et al.A tobacco-derived thymosin β4 concatemer promotes cell proliferation and wound healing in mice[J]. Biomed Research International,2016,2016:1973413.
[22]	KLEINMAN H K,SOSNE G.Thymosin β4 promotes dermal healing[J]. Vitamins and Hormones,2016,102:251-275.
[23]	SHI B,DING Q,HE X,et al.Tβ4-overexpression based on the piggyBac transposon system in cashmere goats alters hair fiber characteristics[J]. Transgenic Research, 2017,26(1):77-85.
[24]	郭栋东.Tβ4转基因绒山羊中目的基因拷贝数及表达量与产绒性能关系的研究[D].呼和浩特:内蒙古大学,2014. GUO D D.The study of target gene copy number/expression level and production performance in Tβ4 transgenic cashmere goats[D].Hohhot:Inner Mongolia University,2014.(in Chinese)
[25]	桓蕾.阿尔巴斯白线山羊胸腺素β4毛囊发育相关信号通路的研究[D].呼和浩特:内蒙古大学,2015. HUAN L.The research of hair follicle developmental related signal pathways of thymosin beta 4 in Arbas cashmere goat[D].Hohhot:Inner Mongolia University,2015.(in Chinese)

Polymorphism and Bioinformatics Analysis of Tβ4 Gene in Qianbei Ma Goat

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References 25

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	WANG Saiqiao, ZHAO Lu, ZHAI Zhenhan, ZHANG Binglei, JIA Wanhang, WANG Yuqin. Tissue Expression and Bioinformatics Analysis of miR-449a/b Precursor Sequences in Sheep [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(6): 2175-2184.
[2]	WEI Mingbang, BIANBA Qiongda, XIAO Qingqing, DUAN Mengqi, CHAMBA Yangzom, SHANG Peng. Cloning,Bioinformatics and Expression Analysis of CDKN1B Gene in Tibetan Pigs [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(6): 2196-2206.
[3]	LI Yongbin, YANG Huilin, SONG Zihao, ZHANG Yu, YANG Jin, MENG Jia, CUI Xiaozhen, LYU Xiaoling, ZHENG Mingxue, BAI Rui. Cloning and Bioinformatics Analysis of MIF Gene in Eimeria tenella [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(6): 2450-2459.
[4]	ZHANG Dianqi, MA Xinhao, YANG Zhimei, MEI Chugang, ZAN Linsen. Bioinformatics Analysis of PAI1 Gene and Its Effect on the Proliferation and Differentiation of Intramuscular Adipocytes in Qinchuan Beef Cattle [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(5): 1729-1741.
[5]	WANG Haorui, YU Baojun, YANG Jiayi, WEI Rong, FU Xi, WANG Chuanchuan, ZHANG Di, LI Desheng, CAI Zhengyun, GU Yaling, ZHANG Juan. Bioinformatics Analysis and Tissue Expression of DERA Gene in Jingyuan Chickens [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(5): 1764-1773.
[6]	CHEN Meiting, ZHENG Congsen, LIANG Zexian, LIN Qiaoer, CHANG Chuanzhe, ZHOU Jun, FENG Jun, LI Linlin, QIN Limei. Cloning and Bioinformatics Analysis of env Gene of Avian Leukosis Virus Subgroup J Strain [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(5): 1785-1795.
[7]	PAN Pengcheng, LEI Zongquan, LI Xian, HU Xiangyun, QIN Qiantao, QIN Zhaoxian, GUAN Zhihui, CHEN Baojian, XIE Bingkun. Bioinformatics Analysis,Eukaryotic Expression Vector Construction and Tissue Expression of MYL2 Gene in Luchuan Pigs [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(5): 1796-1806.
[8]	LIU Jialong, ZHANG Yiwen, ZHANG Yueran, SUN Qijuan, CHEN Jian, HE Lei, JIA Yanyan, DING Ke, YU Zuhua. Cloning,Prokaryotic Expression and Bioinformatics Analysis of ELAVL1 Gene in Chicken [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(5): 1807-1817.
[9]	ZHUO Na, ZHU Zhongwu, LI Jing, HU Shixiang, WANG Jianchang, LIN Hua, CHEN Chaolin, HAN Diangang, AI Jun, CHEN Peifu. Bioinformatics Analysis and Eukaryotic Expression of ORF2 Protein of Swine Hepatitis E Virus [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(5): 1959-1970.
[10]	TAO Qinghua, HUANG Caiyun, CHEN Chao, LIU Wei, SUN Ningning, LI Ang. Cloning and Tissue Expression Difference Analysis of LIF Gene in White Muscovy Duck [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(4): 1296-1306.
[11]	FAN Bingfeng, LI Wen, LIU Lixiang, ZHAO Xiangyuan, SHAO Jing, LIN Lefeng, SUN Huimin, ZHANG Xulin, XU Baozeng. Cloning and Bioinformatics Analysis of AANAT Gene in Mink [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(4): 1307-1318.
[12]	JIA Xinyue, MA Jing, ZHANG Yanyan, MA Xun, WANG Zhengrong, BO Xinwen. Bioinformatics Analysis and Prokaryotic Expression of EGR-07243 and EGR-07243 Genes of Echinococcus granulosus [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(3): 847-858.
[13]	LI Dan, ZHANG Haisen, WANG Yiqun, GAO Dengke, ZHAO Hongcong, LI Chao, JIN Yaping, CHEN Huatao. Eukaryotic Expression Vector Construction,Bioinformatics and Tissue Expression Profiles Analysis of CRY1 Gene in Dairy Cows [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(3): 870-881.
[14]	SONG Xingchao, MENG Jinzhu, ZHAO Yuanyuan, WU Zhenyang, AN Qingming. Cloning and Bioinformatics Analysis of MyoG Gene Promoter Region Sequence in Guizhou White Goat [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(3): 893-903.
[15]	ZHU Xihui, LI Yanlan, WANG Zhiwei, PANG Zheyu, LI Yubao, LIU Cheng, SI Zhenshu, LU Jianbiao, CAO Shengliang, XUE Xijuan. Whole Genome Sequencing of a Resistant Riemerella anatipestifer Strain and Analysis of Antibiotic Resistance and Genetic Evolution [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(3): 1140-1149.