对以纤维素为唯一碳源条件下瘤胃细菌群落动态变化的研究

doi:10.16431/j.cnki.1671-7236.2019.08.016

Abstract

Abstract:

In this experiment, the medium with cellulose as the sole carbon source was used as the growth environment of the rumen bacterial community, and then the 16S rRNA sequence technique was used to analyze the dynamic changes of the rumen bacterial community at different time points.The rumen bacteria were incubated in the media made of anaerobic dilution solution with cellulose for 50 h, and samples were collected at 10, 20, 30, 40 and 50 h, respectively.The DNA samples were extracted and analyzed by 16S rRNA sequencing.The results showed that the Ace of bacteria community was significant different among different time points (P<0.05), and that at 30 h was significantly higher than that of 40 and 50 h (P<0.05).The beta diversity proved that the bacteria community at 40 and 50 h was significant different with that at 10 and 20 h (P<0.05), the bacteria community succession happened in 30 h.The typed rumen cellulolytic bacteria, like Butyrivibrio, Pseudobutyrivibrio, Clostridium, increased with time increasing, but the abundance of Fibrobacter was not significantly changed (P>0.05).With time passing by, the abundances of Acholeplasma, Alkaliphilus, Blautia, GW_34 were increasing and significantly higher at 50 h compared with 10 h (P<0.05) during culture period.In summary, the reference time of enrichment and separation of cellulytic bacteria was 30 to 40 h, and the results provided a theoretical basis for further dilution culture and isolation of new pure cellulytic bacteria.

Key words: rumen; cellulose; dynamic changes

CLC Number:

S823

XING Lei, ZHAO Shengguo, ZHENG Nan, WANG Jiaqi. The Research of Dynamic Changes of Rumen Bacteria Community Cultured in the Medium with Cellulose as the Sole Carbon Source[J]. , 2019, 46(8): 2322-2329.

References 25

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The Research of Dynamic Changes of Rumen Bacteria Community Cultured in the Medium with Cellulose as the Sole Carbon Source

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