尼里-拉菲水牛及海子水牛瘤胃产甲烷菌多样性比较

doi:10.16431/j.cnki.1671-7236.2019.08.013

Abstract

Abstract:

The objective of this study was to investigate the diversity of ruminal Archaea in Nili-Ravi buffaloes and Haizi buffaloes using 16S rDNA library-based analysis.Three female Nili-Ravi buffaloes and three female Haizi buffaloes were used in this study, and ruminal samples were taken by a stomach tube.Total DNA were extracted, and specific primers of Met86F/Met1340R were used to amplify 16S rDNA of methanogens for the construction of library.The results showed that 73 and 100 sequences were obtained from Nili-Ravi buffalos and Haizi buffalos library, respectively.Based the 97% similarity, sequences of Nili-Ravi buffaloes and Haizi buffaloes were assigned to 20 and 33 OTUs, respectively.For Nili-Ravi buffaloes, 70 sequences (17 OTUs) showed ≥ 97% sequence similarity to known species, 3 sequences (3 OTUs) had sequence similarity to known species in the range of 93% to 96%.For Haizi buffaloes, 70 sequences (15 OTUs) showed ≥ 97% sequence similarity to known species, 30 sequences (18 OTUs) had sequence similarity to known species in the range of 84% to 96%.The SGMT and RO clade sequences percentage in Nili-Ravi buffaloes and Haizi buffaloes were 76.71% and 21.91%, 76.00% and 19.00%, respectively.Phylogenetic analysis indicated that these sequences were assigned into two large clades.There were 8 OTUs representative sequences from Nili-Ravi buffaloes and 16 OTUs representative sequences from Haizi buffaloes assigned into the same clade on the top of the phylogenetic tree and far from all known species.It could be concluded that Methanobrevibacter genus was the dominant Archaea in the rumen of Nili-Ravi buffaloes and Haizi buffaloes when fed the same diet.The SGMT clade sequences ratio in Nili-Ravi buffaloes almost were the same with Haizi buffaloes, while the RO clade sequences in Haizi buffaloes were lower than that in Nili-Ravi buffaloes, indicating that there were more unknown methanogen sequences in Haizi buffaloes than that in Nili-Ravi buffaloes.

Key words: Nili-Ravi buffalo; Haizi buffalo; rumen; methanogen; diversity

CLC Number:

S823.8⁺3

YANG Chengjian, LIANG Xianwei, LIANG Xin, TANG Zhenhua, GUO Yanxia, LI Mengwei, PENG Kaiping. Comparative Analysis of Ruminal Archaeal Diversity Between Nili-Ravi Buffaloes and Haizi Buffaloes[J]. , 2019, 46(8): 2296-2306.

References 30

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Comparative Analysis of Ruminal Archaeal Diversity Between Nili-Ravi Buffaloes and Haizi Buffaloes

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