›› 2019, Vol. 46 ›› Issue (8): 2265-2272.doi: 10.16431/j.cnki.1671-7236.2019.08.009

• Physiology and Biochemistry • Previous Articles     Next Articles

Influence of Four Organic Solvents on Viability of Vero Cells and Proliferation of Toxoplasma gondii

XUE Junxin1, XIONG Wei1, ZHANG Qiang1, HUANG Zhongrong1, LI Shuqing1, LI Chunyang1, ZHAO Xinyu1, LI Jian1, WANG Quan2   

  1. 1. Shanghai Entry-Exit Inspection and Quarantine Bureau, Shanghai 200135, China;
    2. Shanghai Veterinary Research Institute, CAAS, Shanghai 200241, China
  • Received:2018-12-21 Online:2019-08-20 Published:2019-08-17

Abstract:

The aim of this study was to screen the organic solvents with lower cytotoxicity and Toxoplasma gondi toxicity.Vero cells were cultured on 96-well plates, the cells were cultured for 12 hours and then the medium was replaced by 200 μL complete DMEM medium containing 0, 0.1%, 0.3%, 0.5%, 1%, 3%, 5% and 10% (V/V) DMSO, dimethyformamide, ethanol and methanol, respectively, blank control holes were set up at the same time.The toxicity of DMSO, dimethylformamide, methanol and ethanol to Vero cells was analyzed by MTT method.The organic solvents with the least cytotoxicity to host cells were screened and the safe concentration was determined.Toxoplasma gondi was inoculated to Vero cells and cultured for 12 hours.The supernatant was discarded and then 120 μL complete DMEM containing 0, 0.1%, 0.3%, 0.5%, 1% and 3% (V/V) DMSO, dimethyformamide, ethanol and methanol was added, respectively.The impacts of solvents on the proliferation of Toxoplasma gondi were determined 36 hours post inoculation.The number of infected cells was counted and the relative infection rate was calculated.The organic solvents with the least impact on the proliferation of Toxoplasma gondi and the safe concentration were screened out.The results showed that there was no obvious cytotoxicity on Vero cells when the volume fraction of DMSO, dimethylformamide, methanol and ethanol were less than 1%, 0.1%, 3% and 1%, respectively.For Toxoplasma gondi, the safety concentrations of DMSO, dimethylformamide, methanol and ethanol were 1%, 0.1%, 1% and 0.1%, respectively.This results indicated DMSO and methanol were less toxic to Vero cells and Toxoplasma gondii, and could be used as organic solvents for the screening of drugs with poor water solubility against Toxoplasma gondii.

Key words: organic solvents; Vero cells; Toxoplasma gondii; vitality; proliferation

CLC Number: