超速离心和酸沉淀制备牛乳清的蛋白质组学研究

doi:10.16431/j.cnki.1671-7236.2019.08.002

Abstract

Abstract:

This study was aimed to analyze whey proteins identified by ultracentrifugation and acid precipitation, and compare the effects of different sample preparation methods on the identification results of whey proteome.Milk samples were collected to remove fat.Whey protein was prepared by ultracentrifugation and acid precipitation, the difference between two methods was compared by LC-MS and database search, the biological processes, cell composition and molecular functions of different proteins were analyzed.Cluster analysis and principal component analysis showed that 53 proteins with increased expression levels were identified by ultracentrifugation, including β-casein, glycosylation-dependent cell adhesion molecule 1 and polyimmunoglobulin receptors;21 proteins with increased expression levels were identified by acid precipitation method, including CD9 antigen, β2-microglobulin and β-lactoglobulin.According to annotation classification, proteins highly expressed in ultracentrifugation were mainly located in extracellular regions, vesicles, intimal system, endoplasmic reticulum and Golgi apparatus, involving protein binding, enzyme-modulating activity, receptor binding and resistance;Proteins highly expressed in acid precipitation were also mainly located in extracellular regions and vesicles, involving binding, nuclease activity, and ionic binding.Based on the above analysis results, the proteome profiles identified by two methods were different, and the combination of two methods could obtain more identified proteins.This study could provide a basis for the selection of sample preparation methods in whey proteomics research.

Key words: milk; whey protein; ultracentrifugation; acid precipitation

CLC Number:

TQ937

QI Yunxia, ZHAO Xiaowei, HUANG Dongwei, CAI Dongdong, YANG Yongxin, CHENG Guanglong, ZHAO Huiling. Proteomics Study on Preparation of Bovine Whey by Ultracentrifugation and Acid Precipitation[J]. , 2019, 46(8): 2203-2210.

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Proteomics Study on Preparation of Bovine Whey by Ultracentrifugation and Acid Precipitation

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