›› 2019, Vol. 46 ›› Issue (5): 1525-1531.doi: 10.16431/j.cnki.1671-7236.2019.05.032

• Basic Veterinary Medicine • Previous Articles     Next Articles

Effect of Low Level Glucose on Proinflammatory Cytokines Expression in Bovine Mammary Epithelial Cells

ZHANG Jinyou1, HUANG He2, LI Wei1, HAN Huansheng1, GAN Wenping1, SONG Weihong1, WEI Ximing1   

  1. 1. Institute of Animal Husbandry and Veterinary of Heilongjiang Academy of Land Reclamation Science, Harbin 150038, China;
    2. Heilongjiang Provincial Center for Disease Control and Prevention, Harbin 150030, China
  • Received:2018-10-10 Online:2019-05-20 Published:2019-05-20

Abstract:

The aim of this study was to investigate the effects of low level glucose on mRNA expression of tumor necrosis factor-α (TNF-α),lysozyme (LYZ),induced nitric oxide synthase (iNOS),interleukin-6 (IL-6),lactoferrin (LF) and interleukin-8 (CXCL8) in bovine mammary epithelial cells.After enzymatic digestion,the bovine mammary epithelial cells were isolated and purified,and then cultured in complete medium containing 0.25,1.00 and 4.50 mg/mL glucose respectively.The expressions of related genes in cells were detected by Real-time fluorescence quantitative PCR.The results showed that there were no significant differences in the relative mRNA expression of the above genes among three glucose groups (P>0.05).The expression levels of TNF-α and LYZ in each concentration group were very low.The expression levels of iNOS were in the middle.However,the expression of IL-6,LF and CXCL8 in different concentration groups was relatively high.It suggested that the decrease of glucose concentration did not directly affect the basic expressions of TNF-α,LYZ,iNOS,IL-6,LF and CXCL8 mRNA in mammary epithelial cells.While without infection,mammary epithelial cells could express a certain amount of iNOS,IL-6,LF and CXCL8,which could be used as a reserve for innate immune defense in order to quickly respond to the invasion of pathogens.

Key words: bovine; mammary epithelial cells; glucose; inflammation

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