›› 2019, Vol. 46 ›› Issue (5): 1447-1455.doi: 10.16431/j.cnki.1671-7236.2019.05.023

• Preventive Veterinary Medicine • Previous Articles     Next Articles

Molecular Epidemiological Investigation on FPV,FAstV and FECoV of Cat in Chengdu

ZHANG Mengwei1, CHEN Yan1, LI Yan2, YANG Xiaonong1, HUANG Jian1   

  1. 1. College of Life Sciences and Technology, Southwest Minzu University, Chengdu 610041, China;
    2. Huapai Bio-engineering Group Co., Ltd., Jianyang 641400, China
  • Received:2018-11-16 Online:2019-05-20 Published:2019-05-20

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Abstract:

To understand the molecular prevalence of feline panleukopenia virus (FPV), feline astrovirus (FAstV) and feline enteric coronavirus (FEcoV) in Chengdu, this experiment collected 315 domestic cats and 34 community stray cats anal swabs containing 228 clinically infected cat samples and 121 clinical healthy cat samples. Viral nucleic acids in all samples were detected by PCR. At the same time, pathogen epidemiology analysis was carried out according to factors such as animal age, living state and immune status, and genetic evolution trees were constructed based on the sequences of viral gene fragments. The results showed that the detection rates of FPV, FAstV and FECoV were 38.4% (134/349), 23.2% (81/349) and 19.5% (68/349), respectively. In the case of mixed infection, FPV/FAstV (9.5%, 33/349), FAstV/FECoV (4.6%, 16/349) and FPV/FECoV (4.3%, 15/349) were the main infections. Based on the analysis of partial gene sequences, the homology of the FPV VP2 gene sequence between samples was 95.1% to 100%. The sick cat strains were divided into two branches, one of which was closely related to the Portuguese strain (GenBank accession No.:KT240136) and the Canadian strain (GenBank accession No.:MF069445), and the other was closely related to the Chinese CPV strain (GenBank accession No.:KY937664).The sequence homology of FAstV ORF1b gene between samples was 90.9% to 99.7%. One of the two branches of the sick cat strain was closely related to the Portuguese strain (GenBank accession No.:KF374704) and the American strain (GenBank accession No.:KM017743). The other branch was closely related to the Hong Kong strain of China (GenBank accession No.:KF499111).The N gene sequence homology of FECoV between samples was 89.3% to 100%, all the FECoV strains sequences were in a branch showing high homology to American (GenBank accession No.:FJ938059),Italy(GenBank accession No.:GU017092/GU017107) and Chinese strain (GenBank accession No.:KT852997).The survey results showed that the situation of cats carrying viral diarrhea in Chengdu was more common, some cats had multiple pathogen mixed infections, and the viral gene sequence had significant variation, increasing the risk of pathogen epidemics and the incidence of poisoned cats. It was necessary to actively carry out clinical prevention and control.

Key words: viral diarrhea; feline panleukopenia virus (FPV); feline astrovirus (FAstV); feline enteric coronavirus (FECoV); genetic variation

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