›› 2019, Vol. 46 ›› Issue (5): 1558-1565.doi: 10.16431/j.cnki.1671-7236.2019.05.036

• Clinical Veterinary Medicine • Previous Articles    

Antibacterial Effect of Different Traditional Chinese Medicines Combined with Enoxacin Hydrochloride on Escherichia coli Resistant Strains in vitro

FU Liyun, CHEN Zhiqiang, GUO Weibing, LI Haihua, ZHANG Pengju, SHANG Jingxue, ZHANG Yuanda   

  1. Inner Mongolia Huatian Pharmaceutical Co., Ltd., Chifeng 024000, China
  • Received:2018-10-23 Online:2019-05-20 Published:2019-05-20

Abstract:

The purpose of this experiment was to study the in vitro bacteriostasis of different kinds of traditional Chinese herbal medicines combined with enrofloxacin hydrochloride against drug-resistant strains of Escherichia coli (E.coli).The minimum inhibitory concentration (MIC) of enrofloxacin hydrochloride (a commonly used drug) and different Chinese herbal medicines against E.coli resistant strains and their combined use was determinated by micro-broth method.The results showed that the antimicrobial effects of Cacumen Biotae,Rosa Laevigata and Schisandra Chinensis Fructus were better in water extracts of single traditional Chinese herbal medicine,and the MIC value were 3.91,3.91 and 7.81 mg/mL,respectively;The antimicrobial effects of Cacumen Biotae and Portulaca Oleracea were better in ethanol extracts of single traditional Chinese herbal medicine,and the MIC value were 7.81 and 3.91 mg/mL,respectively.When enrofloxacin hydrochloride was combined with water extracts of Schisandra Chinensis and Rosa Laevigata,the MIC value of drug-resistant strains of E.coli reduced to 0.0078 and 0.0039 mg/mL,respectively.When enrofloxacin hydrochloride was combined with ethanol extracts of Portulaca Oleracea,Radix Paeoniae Rubra,Cacumen Biotae and Rosa Laevigata,the MIC value of drug-resistant strains significantly decreased.When the enrofloxacin hydrochloride was combined with water extract of Houttuynia Herba,Radix Paeoniae Rubra and Ailanthi Cortex,and alcohol extract of Portulaca Herba,Atractylodis Rhizoma,Berberidis Radix,Paeoniae Radix Rubra,Allii Macrostemi Bulbus,Allii Macrostemonis Bulbus,Platycladi Cacumen,Rosa Laevigatae and Ixeris Denticulata,it showed adding or synergistic bacteriostatic effect on the E.coli resistant strains,the FIC values were 0.75,0.62,0.62,0.27,0.75,0.75,0.31,0.62,0.50,0.53,0.28 and 0.62,respectively.It was preliminarily believed that there were traditional Chinese herbal medicines that could effectively reduce the drug resistance of E.coli among the different kinds of traditional Chinese herbal medicines,there were also traditional Chinese herbal medicines combined with enrofloxacin hydrochloride,which had synergistic or additive effects.It was possible to find effective drug combinations for the prevention and treatment of E.coli disease to against multi-drug-resistant bacteria.

Key words: Escherichia coli; drug resistant strains; enoxacin hydrochloride; traditional Chinese herbal medicines; minimum inhibitory concentration (MIC)

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