›› 2019, Vol. 46 ›› Issue (5): 1516-1524.doi: 10.16431/j.cnki.1671-7236.2019.05.031

• Basic Veterinary Medicine • Previous Articles     Next Articles

Effect of Mitochondria-dependent Pathway on Apoptosis Induced by Zearalenone in Rat Sertoli Cells

ZHU Qihang1,2, SI Mengxue1, LI Xi1, LIU Shuangshuang1, ZOU Hui1, GU Jianhong1, YUAN Yan1, LIU Xuezhong1, LIU Zongping1,2, BIAN Jianchun1,2   

  1. 1. College of Veterinary Medicine, Yangzhou University, Yangzhou 225009, China;
    2. Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou 225009, China
  • Received:2018-09-26 Online:2019-05-20 Published:2019-05-20

Abstract:

In order to elucidate the mechanism of zearalenone (ZEA) on male reproductive toxicity and investigate the pathway of sertoli cell (SC) apoptosis induced by ZEA,the primary SC was used as the experiment material,exposed to different concentration of ZEA(0,5,10,20 μmol/L) for 24 h.qRT-PCR was used to detect the level of gene transcription of Bax and Bcl-2;Western blotting method was used to detect the expression of proteins related to mitochondria-dependent apoptotic pathway.The changes of apoptosis rate and apoptosis-related protein were detected by 20 μmol/L ZEA combined with the inhibitor of Caspase-8 (Z-IETD-FMK).The results showed that compared with the control group,the level of gene transcription of Bcl-2 was decreased significantly in the group treated with 20 μmol/L of ZEA (P<0.05),while the level of gene transcription of Bax increased significantly (P<0.05).The ratio of Bax/Bcl-2 and the expression of Cyto-CytC,Cleaved caspase-3 and Cleaved caspase-9 were extremely significantly increased while the expression of Mito-CytC was decreased extremely significantly in the group treated with 10,20 μmol/L of ZEA (P<0.01).Compared with ZEA group,the apoptosis rate induced by ZEA decreased extremely significantly after being treated with ZEA and Z-IETD-FMK (P<0.01).The ratio of tBid/Bid and Bax/Bcl-2,Cyto-CytC,Cleaved Caspase-3 and Cleaved Caspase-9 were decreased significantly or extremely significantly (P<0.05;P<0.01),while the Mito-CytC protein expression was increased extremely significantly (P<0.01).The results showed that ZEA could induce the apoptosis of SC via the mitochondria-dependent apoptotic pathway.

Key words: zearalenone(ZEA); sertoli cell(SC); mitochondria-dependent apoptotic pathway; inhibitor of Caspase-8

CLC Number: