兔奇异变形杆菌的分离鉴定及耐药性分析

doi:10.16431/j.cnki.1671-7236.2019.05.030

Abstract

Abstract:

In order to understand the cause of diarrhea death in a rabbit field in Lhasa,a strain of bacterium was isolated from the intestinal contents of a rabbit,and was isolated and identified by Gram staining,biochemical identification,16S rRNA PCR amplification and sequence alignment,animal regression test and drug sensitivity test.The results showed that the isolated strain was a single or pair of short rod-shaped Gram-negative bacteria,and the homology of the isolate with Proteus mirabilis in GenBank were 73.5% to 99.8%,the homologous analysis of the 16S rRNA sequence of the isolates and 11 reference strains was carried out by Mega 7.0 software,and the phylogenetic tree was constructed.The isolated strain was identified as Proteus mirabilis,named as T2018.In the animal regression test,there were 3 cases of diarrhea,but none of them died.The results of the necropsy showed that the jejunum and ileum were thin and transparent,with translucent jelly inside;Colon and cecal mucosa were congested,there were bleeding spots on the serosa.Susceptibility tests showed that the isolated strains were only highly sensitive to the five antibacterial agents of amoxicillin/clavulanic acid,gentamicin and ofloxacin,ciprofloxacin and norfloxacin,intermediate to lacillin,and resistant to 24 other antibacterials.The above results indicated that the diarrhea death of rabbits was caused by Proteus mirabilis.

Key words: rabbit; Proteus mirabilis; isolation and identification; drug resistance; β-lactamase

CLC Number:

S852.61

WANG Junshu, XU Jinqiang, JIN Hongyan, HOU Shaohua, FENG Jiawang. Isolation,Identification and Drug Resistance Analysis of Rabbit Proteus mirabilis[J]. , 2019, 46(5): 1508-1515.

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Isolation,Identification and Drug Resistance Analysis of Rabbit Proteus mirabilis

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