内江市猪源大肠埃希菌抗菌药与消毒剂交叉耐药性研究

doi:10.16431/j.cnki.1671-7236.2019.05.029

Abstract

Abstract:

This study was aimed to understand the degree and mechanism of cross resistance of Escherichia coli isolated from some pig farms in Neijiang city to antimicrobials and disinfectants.The minimal inhibitory concentration (MIC) values for enrofloxacin,oxytetracycline,benzalkonium bromide and glutaraldehyde of 66 strains of Escherichia coli isolated from some pig farms in Neijiang city were determined using broth microdilution method and selected cross resistance strains that were resistant to antimicrobials and disinfectants.Whether the cross resistance strains had qacE△1,acrA,acrB and tolC genes was detected by PCR technology and agarose gel electrophoresis.And the mutation of acrA,acrB and tolC genes of these strains were determined.All the 66 strains were resistant to enrofloxacin and oxytetracycline,and three strains (named as No.5,No.6 and No.7) were resistant to benzalkonium bromide,but no strains were resistant to glutaraldehyde.It showed that there were three strains were cross resistance strains.All the three cross resistance strains had acrA,acrB and tolC genes,but only No.6 had qacE△1 gene.All the genes acrA,acrB and tolC of the three strains occurred mutation,and the amino acid that transcribed by these genes also occurred mutation.The highest and lowest base mutation rate were 2.10% (No.7's tolC gene) and 0.97% (No.7's acrA gene).The highest and lowest amino acid mutation rate were 2.39% (No.7's acrB gene) and 0.22% (tolC gene of No.5,No.6 and No.7).And some mutation occurred at the same base site or the same amino acid site.All the 66 strains isolated from some pig farms in Neijiang city were resistant to enrofloxacin and oxytetracycline,and there were cross resistance strains that were resistant to antimicrobials and disinfectants,and the cross resistance mechanism of these strains might related to the efflux pumb AcrAB-TolC.

Key words: Escherichia coli; disinfectant; antimicrobials; cross resistance

CLC Number:

S859.7

YANG Wen, CHEN Hongwei, ZENG Yangmei, WU Junwei. Cross-resistance of Antimicrobials and Disinfectants of Escherichia coli from Neijiang City[J]. , 2019, 46(5): 1499-1507.

References

[1] 李晨,张双翔,周碧君,等.腹泻仔猪肠道致病性大肠埃希氏菌和沙门氏菌的分离鉴定与耐药性研究[J].中国畜牧兽医,2017,44(12):3625-3633. LI C,ZHANG S X,ZHOU B J,et al.Isolation,identification and drug resistance analysis of interstinal pathogenic Escherichia coli and Salmonella isolated form diarrhea piglets[J].China Animal Husbandry & Veterinary Medicine,2017,44(12):3625-3633.(in Chinese)
[2] 王学君,谭艾娟,吕世明,等.贵州省猪源大肠杆菌对四环素类药物的耐药性及耐药基因检测[J].中国畜牧兽医,2018,45(5):1367-1373. WANG X J,TAN A J,LYU S M,et al.Detection of resistance and resistance genes of swine Escherichia coli in Guizhou to tetracyclines[J].China Animal Husbandry & Veterinary Medicine,2018,45(5):1367-1373.(in Chinese)
[3] 陈月月,王凯,王舒丰,等.新疆伊犁地区不同动物源大肠杆菌耐药性调查[J].新疆农业科学,2018,55(8):1560-1568. CHEN Y Y,WANG K,WANG S F,et al.Investigation into drug resistance of Escherichia coli from different animal sources in Yili,Xinjiang[J].Xinjiang Agricultural Sciences,2018,55(8):1560-1568.(in Chinese)
[4] MAVRI A,SMOLE MOZINA S.Development of antimicrobial resistance in Campylobacter jejuni and Campylobacter coli adapted to biocides[J].International Journal of Food Microbiology,2013,160:304-312.
[5] 魏岚.3种常用消毒剂对院内感染常见病原菌效果研究[J].中国病原生物学杂志,2016,11(5):419-423. WEI L.Effect of three commonly used disinfectants on common pathogens responsible for nosocomial infections[J].Journal of Pathogen Biology,2016,11(5):419-423.(in Chinese)
[6] 郭莉娟,何雪梅,邓雯文,等.大熊猫源大肠杆菌及肺炎克雷伯氏菌对消毒剂耐药性研究[J].四川动物,2014,33(6):801-807. GUO L J,HE X M,DENG W W,et al.Disinfectant resistance of Escherichia coli and Klebsiella pneumonia isolated from giant panda[J].Sichuan Journal of Zoology,2014,33(6):801-807.(in Chinese)
[7] 周倩,唐梦君,张小燕,等.禽源弯曲杆菌对消毒剂的耐药性[J].中国兽医学报,2018,38(9):1735-1739. ZHOU Q,TANG M J,ZHANG X Y,et al.Disinfectant resistance of Campylobacter isolated from chicken in Jiangsu province[J].Chinese Journal of Veterinary Science,2018,38(9):1735-1739.(in Chinese)
[8] 马保瑞.大肠杆菌对消毒剂及抗菌药物交叉耐药机制的初步研究[D].重庆:西南大学,2013. MA B R.Preliminary study of Escherichia coli disinfectant and antimicrobial cross-resistance mechanisms[D].Chongqing:Southwest University,2013.(in Chinese)
[9] BUFFET-BATAILLON S,BRANGRE B,CORMIER M,et al.Effect of higher minimum inhibitory concentrations of quatemary ammonium compounds in clinical E. coli isolates on antibiotic susceptibilities and clinical outcomes[J].Journal of Hospital Infection,2011,79(2):141-146.
[10] KVCKEN D,FEUCHT H,KAULFERS P.Association of qacE and qacEΔ1 with multiple resistance to antibiotics and antiseptics in clinical isolates of Gram-negative bacteria[J].Fems Microbiology Letters,2000,183(1):95-98.
[11] HOOPER D C.Emerging mechanisms of fluoroquinolone resistance[J].Emerging Infectious Diseases,2001,7(2):337-341.
[12] PAGÈS J M,MASI M,BARBE J.Inhibitors of efflux pumps in Gram-negative bacteria[J].Trends in Molecular Medicine,2005,11(8):382-389.
[13] HEHR H,REVERDY M E,MABILAT C.et al.Relationship between the level of minimal inhibitory concentration of five antiseptics and the presence of qacA gene in Staphylococcus aureus[J].Pathologie Biologie (Paris),1994,42(5):438-444.
[14] 杨盛智,吴国艳,龙梅,等.蛋鸡场中大肠杆菌对抗生素及消毒剂的耐药性[J].应用与环境生物学报,2017,23(2):312-317. YANG S Z,WU G Y,LONG M,et al.Antibiotic and disinfectant resistance of Escherichia coli isolated from a chicken farm[J].Chinese Journal of Applied and Environmental Biology,2017,23(2):312-317.(in Chinese)
[15] GVNTER K.Adaptive microbial response to low level benzalkonium chloride exposure[J].Journal of Hospital Infection,2018,100(3):e1-e22.
[16] AYA M N,MARWA S M,HEBA H A,et al.The effect of exposure to sub-inhibitory concentrations of hypochlorite and quaternary ammonium compounds on antimicrobial susceptibility of Pseudomonas aeruginosa[J].American Journal of Infection Control,2018,46(7):57-63.
[17] BAY D C,ROMMENS K L,TURNER R J.Small multidrug resistance proteins:A multidrug transporter family that continues to grow[J].Biochim Biophys Acta,2008,1778(9):1814-1838.
[18] PLOY M C,COURVALIN P,LAMBERT T.Characterization of In40 of Enterobacter aerogenes BM2688,a class 1 integron with two new gene cassettes,cmlA2 and qacF[J].Antimicrobial Agents and Chemotherapy,1998,42(10):2557-2563.
[19] LI D,YU T,ZHANG Y,et al.Antibiotic resistance characteristics of environmental bacteria from an oxytetracycline production wastewater treatment plant and the receiving river[J].Applied Environmental Microbiology,2010,76(11):3444-3451.
[20] SMITH K,GEMMELL C G,HUNTER I S.The association between biocide tolerance and the presence or absence of qac genes among hospital-acquired and community-acquired MRSA isolates[J].Journal of Antimicrobial Chemotherapy,2008,61(1):78-84.
[21] 蔺飞.鲍曼不动杆菌耐消毒剂和抗菌药物基因分析及联合抗菌作用研究[D].成都:成都医学院,2018. LIN F.Study on the resistant genes of disinfectant and antimicrobial drug,and combined antibacterial activity in Acinetobacter baumannii[D].Chengdu:Chengdu Medical College,2018.(in Chinese)
[22] 张亚萍,陈勇,王文英,等.临床分离大肠埃希菌耐消毒剂基因携带情况及5种消毒剂最低抑菌浓度[J].中国感染控制杂志,2016,15(5):289-293. ZHANG Y P,CHEN Y,WANG W Y,et al.Carriage of disinfectant resistance genes in clinically isolated Escherichia coli and minimal inhibitory concentration of five disinfectants[J].Chinese Journal of Infection Control,2016,15(5):289-293.(in Chinese)
[23] ROMAO C,MIRANDA C A,SILVA J,et al.Presence of qacEΔ1 gene and susceptibility to a hospital biocide in clinical isolates of Pseudomonas aeruginosa resistant to antibiotics[J]. Curruent Microbiology,2011,63(1):16-21.
[24] 邹立扣,吴国艳,程琳,等.季铵盐类消毒剂及大肠杆菌对其耐药性研究进展[J].食品科学,2014,35(17):338-345. ZOU L K,WU G Y,CHENG L,et al.Progress in research on the resistance of Escherichia coli to quaternary ammonium compounds (QACs)[J].Food Science,2014,35(17):338-345.(in Chinese)
[25] PAGEDAR A,SINGH J,BATISH V K.Efflux mediated adaptive and cross resistance to ciprofloxacin and benzalkonium chloride in Pseudomonas aeruginosa of dairy origin[J].Journal of Basic Microbiology,2011,51(3):289-295.
[26] 于运峰,王风丹.大肠杆菌AcrAB-TolC外输泵的研究进展[J].山东畜牧兽医,2009,30(9):63-65. YU Y F,WANG F D.Research progress on AcrAB-TolC efflux pump of Escherichia coli[J].Shandong Journal of Animal Science and Veterinary Medicine,2009,30(9):63-65.(in Chinese)
[27] LIU J H,PAN Y S,YUAN L,et al.Genetic variations in the active efflux pump genes acrA/B and tolC in different drug-induced strains of Escherichia coli CVCC 1547[J].Genetics & Molecular Research,2013,12(3):2829-2836.
[28] 王秀倩.抗生素耐药性分子机制研究进展[J].口岸卫生控制,2018,23(3):24-31. WANG X Q.Research advances in molecular mechanisms of antibiotic resistance[J].Port Health Control,2018,23(3):24-31.(in Chinese)

Cross-resistance of Antimicrobials and Disinfectants of Escherichia coli from Neijiang City

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