›› 2019, Vol. 46 ›› Issue (5): 1491-1498.doi: 10.16431/j.cnki.1671-7236.2019.05.028

• Basic Veterinary Medicine • Previous Articles     Next Articles

Study on the Effect of Yin-nourishing,Strengthening Immune and Dispelling Evil by Ru Huangxiao Oral Liquid

BAI Dongdong, LI Xinpu, YANG Feng, LUO Jinyin, WANG Xurong, LI Hongsheng   

  1. 1. Key Laboratory of Veterinary Pharmaceutical Discovery, Ministry of Agriculture, Key Laboratory of New Animal Drug Project of Gansu Province, Lanzhou Institute of Animal Husbandry and Pharmaceutical Sciences, Chinese Academy of Agricultural Sciences, Lanzhou 730050, China
  • Received:2018-11-06 Online:2019-05-20 Published:2019-05-20

Abstract:

To explore the effects of Ru Huangxiao oral liquid on yin-nourishing, strengthening righteousness and eliminating evil spirits, this study observed the clinical symptoms,diet,water consumption, body weight, thymus index, spleen index, contents of total triiodothyronine (T3), total thyroxine (T4) and thyroid stimulating hormone (TSH) in rats with yin deficiency model and the effect of mouse macrophage phagocytosis and inhibition on Escherichia coli, Staphylococcus aureus, Streptococcus agalactiae and Streptococcus uberis. The results showed that the rats of yin deficiency model had normal mental state and were easy to grasp after the administration of Ru Huangxiao oral liquid, but the gloss of the coat was still poor, there was hair removal and the stool was normal. Compared with model group, the body weight (P>0.05), thymus index (P>0.05), spleen index (P>0.05), TSH content (P<0.05) of the experimental group increased, the food intake (P<0.05), T3 (P<0.05) and T4 content (P>0.05) decreased. However, the difference was not significantly different from the negative control group (P>0.05).Compared with model group, the water consumption of the rats in the experimental group significantly decreased (P<0.05), but it was still significantly higher than the negative control group (P<0.05). The phagocytic index of the high, middle and low dose groups and the negative control group were significantly different (P<0.05). The percentage of phagocytosis was significantly higher in the high-dose and middle-dose groups compared with the negative control group (P<0.05). The minimum inhibitory concentration (MIC) of Ru Huangxiao oral liquid to Escherichia coli, Staphylococcus aureus, Streptococcus agalactiae and Streptococcus parauberis were 12.50, 1.56, 0.20 and 0.39 mg/mL, respectively. In summary, the Ru Huangxiao oral liquid could improve the clinical symptoms of yin deficiency in rats and enhance the phagocytic function of macrophages. At the same time, it could inhibit the main pathogens of cow mastitis, and had the effects of nourishing yin, strengthening righteousness and eliminating evil spirits, it could be used for the treatment of dairy cow mastitis.

Key words: Ru Huangxiao oral liquid; model of yin deficiency; macrophage; antibacterial activity

CLC Number: