吉林省猪圆环病毒3型的分子流行病学调查及遗传演化分析

doi:10.16431/j.cnki.1671-7236.2019.05.027

Abstract

Abstract:

To understand the prevalence and molecular biology of porcine circovirus type 3 (PCV3) in Jilin province,a total of 484 serum samples in Jilin province from 2015 to 2017 were detected by PCR in this study.The ORF2 gene sequence of PCV3 positive samples was sequenced,the molecular characterization of ORF2 gene sequences was analyzed using bioinformatics softwares of DNAStar and Mega 6.06.The results showed that the total infection rates of PCV3 were 28.1% (136/484) at serum samples and 65.8% (25/38) at pig farms,and showed increased infection rates from 2015 to 2017.The homology alignment analysis showed that the nucleotide and amino acid homology of the 4 ORF2 gene sequences in this study shared 98.3% to 98.9% and 97.7% to 99.5% homologies,respectively,and also shared 97.7% to 99.7% and 96.7% to 100% homologies in the nucleotide and amino acid with reference ORF2 gene sequences in GenBank.The results of phylogenetic showed that there were two major branches of PCV3 strains,PCV3a and PCV3b.The PCV3 strains isolated in Jilin province were distributed on the two major branches,PCV3a (1 isolate) and PCV3b (3 isolates).The difference between the amino acid at sites 24 (A,V) and 27 (R,K) of the Cap protein might be generated during the evolutionary of PCV3 strains.These results showed that PCV3 had a high infection rates in pig herds and pig farms in Jilin province.The molecular characterization of PCV3 showed that PCV3 strains were highly conserved in natural evolution.This study provided an important reference for the molecular characteristics of PCV3.

Key words: porcine circovirus type 3 (PCV3); ORF2 gene; molecular epidemiology; genetic characterization

CLC Number:

S852.65

GUO Yingcheng, FU Shoupeng, GAO Xueyong, LI Xudong. Molecular Epidemiological Investigation and Genetic Evolution Analysis of Porcine Circovirus Type 3 in Jilin Province[J]. , 2019, 46(5): 1483-1490.

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Molecular Epidemiological Investigation and Genetic Evolution Analysis of Porcine Circovirus Type 3 in Jilin Province

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